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The Synthesis of Ascorbic Acid in Rice Roots Plays an Important Role in the Salt Tolerance of Rice by Scavenging ROS

The root plays an important role in the responses of plants to stresses, but the detailed mechanisms of roots in stress responses are still obscure. The GDP-mannose pyrophosphate synthetase (GMPase) OsVTC1-3 is a key factor of ascorbic acid (AsA) synthesis in rice roots. The present study showed tha...

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Autores principales: Wang, Yayun, Zhao, Hui, Qin, Hua, Li, Zixuan, Liu, Hai, Wang, Juan, Zhang, Haiwen, Quan, Ruidang, Huang, Rongfeng, Zhang, Zhijin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275051/
https://www.ncbi.nlm.nih.gov/pubmed/30373162
http://dx.doi.org/10.3390/ijms19113347
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author Wang, Yayun
Zhao, Hui
Qin, Hua
Li, Zixuan
Liu, Hai
Wang, Juan
Zhang, Haiwen
Quan, Ruidang
Huang, Rongfeng
Zhang, Zhijin
author_facet Wang, Yayun
Zhao, Hui
Qin, Hua
Li, Zixuan
Liu, Hai
Wang, Juan
Zhang, Haiwen
Quan, Ruidang
Huang, Rongfeng
Zhang, Zhijin
author_sort Wang, Yayun
collection PubMed
description The root plays an important role in the responses of plants to stresses, but the detailed mechanisms of roots in stress responses are still obscure. The GDP-mannose pyrophosphate synthetase (GMPase) OsVTC1-3 is a key factor of ascorbic acid (AsA) synthesis in rice roots. The present study showed that the transcript of OsVTC1-3 was induced by salt stress in roots, but not in leaves. Inhibiting the expression of OsVTC1-3 by RNA interfering (RI) technology significantly impaired the tolerance of rice to salt stress. The roots of OsVTC1-3 RI plants rapidly produced more O(2)(−), and later accumulated amounts of H(2)O(2) under salt stress, indicating the impaired tolerance of OsVTC1-3 RI plants to salt stress due to the decreasing ability of scavenging reactive oxygen species (ROS). Moreover, exogenous AsA restored the salt tolerance of OsVTC1-3 RI plants, indicating that the AsA synthesis in rice roots is an important factor for the response of rice to salt stress. Further studies showed that the salt-induced AsA synthesis was limited in the roots of OsVTC1-3 RI plants. The above results showed that specifically regulating AsA synthesis to scavenge ROS in rice roots was one of important factors in enhancing the tolerance of rice to salt stress.
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spelling pubmed-62750512018-12-15 The Synthesis of Ascorbic Acid in Rice Roots Plays an Important Role in the Salt Tolerance of Rice by Scavenging ROS Wang, Yayun Zhao, Hui Qin, Hua Li, Zixuan Liu, Hai Wang, Juan Zhang, Haiwen Quan, Ruidang Huang, Rongfeng Zhang, Zhijin Int J Mol Sci Article The root plays an important role in the responses of plants to stresses, but the detailed mechanisms of roots in stress responses are still obscure. The GDP-mannose pyrophosphate synthetase (GMPase) OsVTC1-3 is a key factor of ascorbic acid (AsA) synthesis in rice roots. The present study showed that the transcript of OsVTC1-3 was induced by salt stress in roots, but not in leaves. Inhibiting the expression of OsVTC1-3 by RNA interfering (RI) technology significantly impaired the tolerance of rice to salt stress. The roots of OsVTC1-3 RI plants rapidly produced more O(2)(−), and later accumulated amounts of H(2)O(2) under salt stress, indicating the impaired tolerance of OsVTC1-3 RI plants to salt stress due to the decreasing ability of scavenging reactive oxygen species (ROS). Moreover, exogenous AsA restored the salt tolerance of OsVTC1-3 RI plants, indicating that the AsA synthesis in rice roots is an important factor for the response of rice to salt stress. Further studies showed that the salt-induced AsA synthesis was limited in the roots of OsVTC1-3 RI plants. The above results showed that specifically regulating AsA synthesis to scavenge ROS in rice roots was one of important factors in enhancing the tolerance of rice to salt stress. MDPI 2018-10-26 /pmc/articles/PMC6275051/ /pubmed/30373162 http://dx.doi.org/10.3390/ijms19113347 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yayun
Zhao, Hui
Qin, Hua
Li, Zixuan
Liu, Hai
Wang, Juan
Zhang, Haiwen
Quan, Ruidang
Huang, Rongfeng
Zhang, Zhijin
The Synthesis of Ascorbic Acid in Rice Roots Plays an Important Role in the Salt Tolerance of Rice by Scavenging ROS
title The Synthesis of Ascorbic Acid in Rice Roots Plays an Important Role in the Salt Tolerance of Rice by Scavenging ROS
title_full The Synthesis of Ascorbic Acid in Rice Roots Plays an Important Role in the Salt Tolerance of Rice by Scavenging ROS
title_fullStr The Synthesis of Ascorbic Acid in Rice Roots Plays an Important Role in the Salt Tolerance of Rice by Scavenging ROS
title_full_unstemmed The Synthesis of Ascorbic Acid in Rice Roots Plays an Important Role in the Salt Tolerance of Rice by Scavenging ROS
title_short The Synthesis of Ascorbic Acid in Rice Roots Plays an Important Role in the Salt Tolerance of Rice by Scavenging ROS
title_sort synthesis of ascorbic acid in rice roots plays an important role in the salt tolerance of rice by scavenging ros
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275051/
https://www.ncbi.nlm.nih.gov/pubmed/30373162
http://dx.doi.org/10.3390/ijms19113347
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