Cargando…
The effect of insulin on equine lamellar basal epithelial cells mediated by the insulin-like growth factor-1 receptor
BACKGROUND: In horses and ponies, insulin dysregulation leading to hyperinsulinemia may be associated with increased risk of laminitis, and prolonged infusion of insulin can induce the condition. It is unclear whether insulin may have a direct or indirect effect on the lamellar tissues. Insulin is s...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275117/ https://www.ncbi.nlm.nih.gov/pubmed/30519508 http://dx.doi.org/10.7717/peerj.5945 |
_version_ | 1783377760993935360 |
---|---|
author | Baskerville, Courtnay L. Chockalingham, Subu Harris, Patricia A. Bailey, Simon R. |
author_facet | Baskerville, Courtnay L. Chockalingham, Subu Harris, Patricia A. Bailey, Simon R. |
author_sort | Baskerville, Courtnay L. |
collection | PubMed |
description | BACKGROUND: In horses and ponies, insulin dysregulation leading to hyperinsulinemia may be associated with increased risk of laminitis, and prolonged infusion of insulin can induce the condition. It is unclear whether insulin may have a direct or indirect effect on the lamellar tissues. Insulin is structurally related to insulin-like growth factor (IGF-1), and can bind the IGF-1 receptor, albeit at a lower affinity than IGF-1. METHODS: Immunohistochemistry was performed on formalin-fixed lamellar tissue sections from six normal horses, euthanised for non-research purposes, using an anti-IGF-1 receptor antibody. In further studies, lamellar epithelial cells were obtained by collagenase digestion from the hooves of 18 normal horses, also euthanised for non-research purposes, and incubated for 48 h in the presence of insulin (0–2,000 m IU/ml). The increase in cell numbers was determined using a cell proliferation assay, and compared to the effect of zero insulin using one-way ANOVA. RESULTS: Immunohistochemistry demonstrated IGF-1 receptors on lamellar epidermal epithelial cells. With cultured cells, insulin caused a concentration-dependent increase in cell proliferation compared to untreated cells (maximal effect 63.3 ± 12.8% more cells after 48 h with 1,000 m IU/ml insulin; P < 0.01). Co-incubation with a blocking antibody against the IGF-1 receptor significantly inhibited the proliferative effect of insulin (P < 0.01). DISCUSSION: These results demonstrate that IGF-1 receptors are present on lamellar epithelial cells. At high physiological concentrations, insulin may activate these cells, by a mechanism involving IGF-1 receptors, resulting in a proliferative effect. This mechanism could help to explain the link between hyperinsulinemia and laminitis. |
format | Online Article Text |
id | pubmed-6275117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62751172018-12-05 The effect of insulin on equine lamellar basal epithelial cells mediated by the insulin-like growth factor-1 receptor Baskerville, Courtnay L. Chockalingham, Subu Harris, Patricia A. Bailey, Simon R. PeerJ Cell Biology BACKGROUND: In horses and ponies, insulin dysregulation leading to hyperinsulinemia may be associated with increased risk of laminitis, and prolonged infusion of insulin can induce the condition. It is unclear whether insulin may have a direct or indirect effect on the lamellar tissues. Insulin is structurally related to insulin-like growth factor (IGF-1), and can bind the IGF-1 receptor, albeit at a lower affinity than IGF-1. METHODS: Immunohistochemistry was performed on formalin-fixed lamellar tissue sections from six normal horses, euthanised for non-research purposes, using an anti-IGF-1 receptor antibody. In further studies, lamellar epithelial cells were obtained by collagenase digestion from the hooves of 18 normal horses, also euthanised for non-research purposes, and incubated for 48 h in the presence of insulin (0–2,000 m IU/ml). The increase in cell numbers was determined using a cell proliferation assay, and compared to the effect of zero insulin using one-way ANOVA. RESULTS: Immunohistochemistry demonstrated IGF-1 receptors on lamellar epidermal epithelial cells. With cultured cells, insulin caused a concentration-dependent increase in cell proliferation compared to untreated cells (maximal effect 63.3 ± 12.8% more cells after 48 h with 1,000 m IU/ml insulin; P < 0.01). Co-incubation with a blocking antibody against the IGF-1 receptor significantly inhibited the proliferative effect of insulin (P < 0.01). DISCUSSION: These results demonstrate that IGF-1 receptors are present on lamellar epithelial cells. At high physiological concentrations, insulin may activate these cells, by a mechanism involving IGF-1 receptors, resulting in a proliferative effect. This mechanism could help to explain the link between hyperinsulinemia and laminitis. PeerJ Inc. 2018-11-29 /pmc/articles/PMC6275117/ /pubmed/30519508 http://dx.doi.org/10.7717/peerj.5945 Text en © 2018 Baskerville et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Cell Biology Baskerville, Courtnay L. Chockalingham, Subu Harris, Patricia A. Bailey, Simon R. The effect of insulin on equine lamellar basal epithelial cells mediated by the insulin-like growth factor-1 receptor |
title | The effect of insulin on equine lamellar basal epithelial cells mediated by the insulin-like growth factor-1 receptor |
title_full | The effect of insulin on equine lamellar basal epithelial cells mediated by the insulin-like growth factor-1 receptor |
title_fullStr | The effect of insulin on equine lamellar basal epithelial cells mediated by the insulin-like growth factor-1 receptor |
title_full_unstemmed | The effect of insulin on equine lamellar basal epithelial cells mediated by the insulin-like growth factor-1 receptor |
title_short | The effect of insulin on equine lamellar basal epithelial cells mediated by the insulin-like growth factor-1 receptor |
title_sort | effect of insulin on equine lamellar basal epithelial cells mediated by the insulin-like growth factor-1 receptor |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275117/ https://www.ncbi.nlm.nih.gov/pubmed/30519508 http://dx.doi.org/10.7717/peerj.5945 |
work_keys_str_mv | AT baskervillecourtnayl theeffectofinsulinonequinelamellarbasalepithelialcellsmediatedbytheinsulinlikegrowthfactor1receptor AT chockalinghamsubu theeffectofinsulinonequinelamellarbasalepithelialcellsmediatedbytheinsulinlikegrowthfactor1receptor AT harrispatriciaa theeffectofinsulinonequinelamellarbasalepithelialcellsmediatedbytheinsulinlikegrowthfactor1receptor AT baileysimonr theeffectofinsulinonequinelamellarbasalepithelialcellsmediatedbytheinsulinlikegrowthfactor1receptor AT baskervillecourtnayl effectofinsulinonequinelamellarbasalepithelialcellsmediatedbytheinsulinlikegrowthfactor1receptor AT chockalinghamsubu effectofinsulinonequinelamellarbasalepithelialcellsmediatedbytheinsulinlikegrowthfactor1receptor AT harrispatriciaa effectofinsulinonequinelamellarbasalepithelialcellsmediatedbytheinsulinlikegrowthfactor1receptor AT baileysimonr effectofinsulinonequinelamellarbasalepithelialcellsmediatedbytheinsulinlikegrowthfactor1receptor |