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Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis

PISTILLATA (PI) homologs are crucial regulators of flower development in angiosperms. In this study, we isolated the MAwuPI homolog from Magnolia wufengensis, a basal angiosperm belonging to the Magnoliaceae. Molecular phylogenetic analysis suggested that MAwuPI was grouped into the PI/GLO lineages...

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Autores principales: Liu, Wen, Shen, Xiangling, Liang, Hongwei, Wang, Yubing, He, Zhengquan, Zhang, Dechun, Chen, Faju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275295/
https://www.ncbi.nlm.nih.gov/pubmed/30534136
http://dx.doi.org/10.3389/fpls.2018.01743
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author Liu, Wen
Shen, Xiangling
Liang, Hongwei
Wang, Yubing
He, Zhengquan
Zhang, Dechun
Chen, Faju
author_facet Liu, Wen
Shen, Xiangling
Liang, Hongwei
Wang, Yubing
He, Zhengquan
Zhang, Dechun
Chen, Faju
author_sort Liu, Wen
collection PubMed
description PISTILLATA (PI) homologs are crucial regulators of flower development in angiosperms. In this study, we isolated the MAwuPI homolog from Magnolia wufengensis, a basal angiosperm belonging to the Magnoliaceae. Molecular phylogenetic analysis suggested that MAwuPI was grouped into the PI/GLO lineages of B-class MADS-box gene with the distinctive PI motif. Further expression profiling analysis showed that MAwuPI was expressed in tepals and stamens but not in juvenile leaves and carpels, similar to the spatial expression pattern of AtPI in Arabidopsis. Interestingly, MAwuPI had higher expression level in inner-tepals than in outer-tepals, whereas the M. wufengensis flower is homochlamydeous. Moreover, ectopic expression of MAwuPI in Arabidopsis pi-1 mutant emerged filament-like structures but had no obvious petals, suggesting a partial phenotypic recovery of pi-1 mutant. The features of MAwuPI in the expression pattern and gene function improved our acknowledgment of B-class genes in M. wufengensis, and contributed to the clarification of M. wufengensis evolution status and relations with other sibling species in molecular perspective.
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spelling pubmed-62752952018-12-10 Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis Liu, Wen Shen, Xiangling Liang, Hongwei Wang, Yubing He, Zhengquan Zhang, Dechun Chen, Faju Front Plant Sci Plant Science PISTILLATA (PI) homologs are crucial regulators of flower development in angiosperms. In this study, we isolated the MAwuPI homolog from Magnolia wufengensis, a basal angiosperm belonging to the Magnoliaceae. Molecular phylogenetic analysis suggested that MAwuPI was grouped into the PI/GLO lineages of B-class MADS-box gene with the distinctive PI motif. Further expression profiling analysis showed that MAwuPI was expressed in tepals and stamens but not in juvenile leaves and carpels, similar to the spatial expression pattern of AtPI in Arabidopsis. Interestingly, MAwuPI had higher expression level in inner-tepals than in outer-tepals, whereas the M. wufengensis flower is homochlamydeous. Moreover, ectopic expression of MAwuPI in Arabidopsis pi-1 mutant emerged filament-like structures but had no obvious petals, suggesting a partial phenotypic recovery of pi-1 mutant. The features of MAwuPI in the expression pattern and gene function improved our acknowledgment of B-class genes in M. wufengensis, and contributed to the clarification of M. wufengensis evolution status and relations with other sibling species in molecular perspective. Frontiers Media S.A. 2018-11-26 /pmc/articles/PMC6275295/ /pubmed/30534136 http://dx.doi.org/10.3389/fpls.2018.01743 Text en Copyright © 2018 Liu, Shen, Liang, Wang, He, Zhang and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liu, Wen
Shen, Xiangling
Liang, Hongwei
Wang, Yubing
He, Zhengquan
Zhang, Dechun
Chen, Faju
Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis
title Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis
title_full Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis
title_fullStr Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis
title_full_unstemmed Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis
title_short Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis
title_sort isolation and functional analysis of pistillata homolog from magnolia wufengensis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275295/
https://www.ncbi.nlm.nih.gov/pubmed/30534136
http://dx.doi.org/10.3389/fpls.2018.01743
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