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Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis
PISTILLATA (PI) homologs are crucial regulators of flower development in angiosperms. In this study, we isolated the MAwuPI homolog from Magnolia wufengensis, a basal angiosperm belonging to the Magnoliaceae. Molecular phylogenetic analysis suggested that MAwuPI was grouped into the PI/GLO lineages...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275295/ https://www.ncbi.nlm.nih.gov/pubmed/30534136 http://dx.doi.org/10.3389/fpls.2018.01743 |
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author | Liu, Wen Shen, Xiangling Liang, Hongwei Wang, Yubing He, Zhengquan Zhang, Dechun Chen, Faju |
author_facet | Liu, Wen Shen, Xiangling Liang, Hongwei Wang, Yubing He, Zhengquan Zhang, Dechun Chen, Faju |
author_sort | Liu, Wen |
collection | PubMed |
description | PISTILLATA (PI) homologs are crucial regulators of flower development in angiosperms. In this study, we isolated the MAwuPI homolog from Magnolia wufengensis, a basal angiosperm belonging to the Magnoliaceae. Molecular phylogenetic analysis suggested that MAwuPI was grouped into the PI/GLO lineages of B-class MADS-box gene with the distinctive PI motif. Further expression profiling analysis showed that MAwuPI was expressed in tepals and stamens but not in juvenile leaves and carpels, similar to the spatial expression pattern of AtPI in Arabidopsis. Interestingly, MAwuPI had higher expression level in inner-tepals than in outer-tepals, whereas the M. wufengensis flower is homochlamydeous. Moreover, ectopic expression of MAwuPI in Arabidopsis pi-1 mutant emerged filament-like structures but had no obvious petals, suggesting a partial phenotypic recovery of pi-1 mutant. The features of MAwuPI in the expression pattern and gene function improved our acknowledgment of B-class genes in M. wufengensis, and contributed to the clarification of M. wufengensis evolution status and relations with other sibling species in molecular perspective. |
format | Online Article Text |
id | pubmed-6275295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62752952018-12-10 Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis Liu, Wen Shen, Xiangling Liang, Hongwei Wang, Yubing He, Zhengquan Zhang, Dechun Chen, Faju Front Plant Sci Plant Science PISTILLATA (PI) homologs are crucial regulators of flower development in angiosperms. In this study, we isolated the MAwuPI homolog from Magnolia wufengensis, a basal angiosperm belonging to the Magnoliaceae. Molecular phylogenetic analysis suggested that MAwuPI was grouped into the PI/GLO lineages of B-class MADS-box gene with the distinctive PI motif. Further expression profiling analysis showed that MAwuPI was expressed in tepals and stamens but not in juvenile leaves and carpels, similar to the spatial expression pattern of AtPI in Arabidopsis. Interestingly, MAwuPI had higher expression level in inner-tepals than in outer-tepals, whereas the M. wufengensis flower is homochlamydeous. Moreover, ectopic expression of MAwuPI in Arabidopsis pi-1 mutant emerged filament-like structures but had no obvious petals, suggesting a partial phenotypic recovery of pi-1 mutant. The features of MAwuPI in the expression pattern and gene function improved our acknowledgment of B-class genes in M. wufengensis, and contributed to the clarification of M. wufengensis evolution status and relations with other sibling species in molecular perspective. Frontiers Media S.A. 2018-11-26 /pmc/articles/PMC6275295/ /pubmed/30534136 http://dx.doi.org/10.3389/fpls.2018.01743 Text en Copyright © 2018 Liu, Shen, Liang, Wang, He, Zhang and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Liu, Wen Shen, Xiangling Liang, Hongwei Wang, Yubing He, Zhengquan Zhang, Dechun Chen, Faju Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis |
title | Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis |
title_full | Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis |
title_fullStr | Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis |
title_full_unstemmed | Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis |
title_short | Isolation and Functional Analysis of PISTILLATA Homolog From Magnolia wufengensis |
title_sort | isolation and functional analysis of pistillata homolog from magnolia wufengensis |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275295/ https://www.ncbi.nlm.nih.gov/pubmed/30534136 http://dx.doi.org/10.3389/fpls.2018.01743 |
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