Cargando…

Comparison of miRNA Profiles of Cord Blood Stem Cells in Identical and Fraternal Twins

OBJECTIVE: The role of epigenetic in regulating of the gene expression profile the embryo has been documented. MicroRNAs (miRNAs) are one of these epigenetic mechanisms. Twins are valuable models in determining the relative contributions of genetics and the environment. In this study, we compared di...

Descripción completa

Detalles Bibliográficos
Autores principales: Ajami, Monireh, Sadeghian, Mohammad Hadi, Soleimani, Masoud, Keramati, Mohammad Reza, Ajami, Mansoureh, Anbarlou, Azadeh, Atashi, Amir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275421/
https://www.ncbi.nlm.nih.gov/pubmed/30507092
http://dx.doi.org/10.22074/cellj.2019.5683
_version_ 1783377809374183424
author Ajami, Monireh
Sadeghian, Mohammad Hadi
Soleimani, Masoud
Keramati, Mohammad Reza
Ajami, Mansoureh
Anbarlou, Azadeh
Atashi, Amir
author_facet Ajami, Monireh
Sadeghian, Mohammad Hadi
Soleimani, Masoud
Keramati, Mohammad Reza
Ajami, Mansoureh
Anbarlou, Azadeh
Atashi, Amir
author_sort Ajami, Monireh
collection PubMed
description OBJECTIVE: The role of epigenetic in regulating of the gene expression profile the embryo has been documented. MicroRNAs (miRNAs) are one of these epigenetic mechanisms. Twins are valuable models in determining the relative contributions of genetics and the environment. In this study, we compared differences in the expression levels of 44 miRNAs in hematopoietic stem cells (HSCs) of identical twins to that of fraternal twins as a controls. MATERIALS AND METHODS: In this experimental study, CD133(+) HSCs were isolated from cord blood of identical and fraternal twins via magnetic-activated cell sorting (MACS). Variation in of gene expression levels of 44 miRNAs were evaluated using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). RESULTS: Significant differences in expression were observed in both fraternal and identical twins to varying degrees, but variations alteration in expression of the miRNAs were higher in fraternal twins. CONCLUSION: Identical twins had a positive correlation in miRNA expression, while the correlation was not statistically significant in fraternal twins. Altogether, more differences in miRNA expression level in fraternal twins can be attributed to the both genetics and the intrauterine environment. The contribution of the intrauterine environment and genetics to miRNAs expression in HSCs was estimated 8 and 92%, respectively. By comparing of miRNA expression in identical and fraternal twins and identification of their target genes and biological pathways, it could be possible to estimate the effects of genetics and the environment on a number of biological pathways.
format Online
Article
Text
id pubmed-6275421
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Royan Institute
record_format MEDLINE/PubMed
spelling pubmed-62754212019-04-01 Comparison of miRNA Profiles of Cord Blood Stem Cells in Identical and Fraternal Twins Ajami, Monireh Sadeghian, Mohammad Hadi Soleimani, Masoud Keramati, Mohammad Reza Ajami, Mansoureh Anbarlou, Azadeh Atashi, Amir Cell J Original Article OBJECTIVE: The role of epigenetic in regulating of the gene expression profile the embryo has been documented. MicroRNAs (miRNAs) are one of these epigenetic mechanisms. Twins are valuable models in determining the relative contributions of genetics and the environment. In this study, we compared differences in the expression levels of 44 miRNAs in hematopoietic stem cells (HSCs) of identical twins to that of fraternal twins as a controls. MATERIALS AND METHODS: In this experimental study, CD133(+) HSCs were isolated from cord blood of identical and fraternal twins via magnetic-activated cell sorting (MACS). Variation in of gene expression levels of 44 miRNAs were evaluated using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). RESULTS: Significant differences in expression were observed in both fraternal and identical twins to varying degrees, but variations alteration in expression of the miRNAs were higher in fraternal twins. CONCLUSION: Identical twins had a positive correlation in miRNA expression, while the correlation was not statistically significant in fraternal twins. Altogether, more differences in miRNA expression level in fraternal twins can be attributed to the both genetics and the intrauterine environment. The contribution of the intrauterine environment and genetics to miRNAs expression in HSCs was estimated 8 and 92%, respectively. By comparing of miRNA expression in identical and fraternal twins and identification of their target genes and biological pathways, it could be possible to estimate the effects of genetics and the environment on a number of biological pathways. Royan Institute 2019 2018-11-18 /pmc/articles/PMC6275421/ /pubmed/30507092 http://dx.doi.org/10.22074/cellj.2019.5683 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ajami, Monireh
Sadeghian, Mohammad Hadi
Soleimani, Masoud
Keramati, Mohammad Reza
Ajami, Mansoureh
Anbarlou, Azadeh
Atashi, Amir
Comparison of miRNA Profiles of Cord Blood Stem Cells in Identical and Fraternal Twins
title Comparison of miRNA Profiles of Cord Blood Stem Cells in Identical and Fraternal Twins
title_full Comparison of miRNA Profiles of Cord Blood Stem Cells in Identical and Fraternal Twins
title_fullStr Comparison of miRNA Profiles of Cord Blood Stem Cells in Identical and Fraternal Twins
title_full_unstemmed Comparison of miRNA Profiles of Cord Blood Stem Cells in Identical and Fraternal Twins
title_short Comparison of miRNA Profiles of Cord Blood Stem Cells in Identical and Fraternal Twins
title_sort comparison of mirna profiles of cord blood stem cells in identical and fraternal twins
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275421/
https://www.ncbi.nlm.nih.gov/pubmed/30507092
http://dx.doi.org/10.22074/cellj.2019.5683
work_keys_str_mv AT ajamimonireh comparisonofmirnaprofilesofcordbloodstemcellsinidenticalandfraternaltwins
AT sadeghianmohammadhadi comparisonofmirnaprofilesofcordbloodstemcellsinidenticalandfraternaltwins
AT soleimanimasoud comparisonofmirnaprofilesofcordbloodstemcellsinidenticalandfraternaltwins
AT keramatimohammadreza comparisonofmirnaprofilesofcordbloodstemcellsinidenticalandfraternaltwins
AT ajamimansoureh comparisonofmirnaprofilesofcordbloodstemcellsinidenticalandfraternaltwins
AT anbarlouazadeh comparisonofmirnaprofilesofcordbloodstemcellsinidenticalandfraternaltwins
AT atashiamir comparisonofmirnaprofilesofcordbloodstemcellsinidenticalandfraternaltwins