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DU-145 prostate carcinoma cells that selectively transmigrate narrow obstacles express elevated levels of Cx43

The formation of aqueous intercellular channels mediating gap junctional intercellular coupling (GJIC) is a canonical function of connexins (Cx). In contrast, mechanisms of GJIC-independent involvement of connexins in cancer formation and metastasis remain a matter of debate. Because of the role of...

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Autores principales: Szpak, Katarzyna, Wybieralska, Ewa, Niedziałkowska, Ewa, Rak, Monika, Bechyne, Iga, Michalik, Marta, Madeja, Zbigniew, Czyż, Jarosław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SP Versita 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275569/
https://www.ncbi.nlm.nih.gov/pubmed/21910090
http://dx.doi.org/10.2478/s11658-011-0027-7
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author Szpak, Katarzyna
Wybieralska, Ewa
Niedziałkowska, Ewa
Rak, Monika
Bechyne, Iga
Michalik, Marta
Madeja, Zbigniew
Czyż, Jarosław
author_facet Szpak, Katarzyna
Wybieralska, Ewa
Niedziałkowska, Ewa
Rak, Monika
Bechyne, Iga
Michalik, Marta
Madeja, Zbigniew
Czyż, Jarosław
author_sort Szpak, Katarzyna
collection PubMed
description The formation of aqueous intercellular channels mediating gap junctional intercellular coupling (GJIC) is a canonical function of connexins (Cx). In contrast, mechanisms of GJIC-independent involvement of connexins in cancer formation and metastasis remain a matter of debate. Because of the role of Cx43 in the determination of carcinoma cell invasive potential, we addressed the problem of the possible Cx43 involvement in early prostate cancer invasion. For this purpose, we analysed Cx43-positive DU-145 cell subsets established from the progenies of the cells most readily transmigrating microporous membranes. These progenies displayed motile activity similar to the control DU-145 cells but were characterized by elevated Cx43 expression levels and GJIC intensity. Thus, apparent links exist between Cx43 expression and transmigration potential of DU-145 cells. Moreover, Cx43 expression profiles in the analysed DU-145 subsets were not affected by intercellular contacts and chemical inhibition of GJIC during the transmigration. Our observations indicate that neither cell motility nor GJIC determines the transmigration efficiency of DU-145 cells. However, we postulate that selective transmigration of prostate cancer cells expressing elevated levels of Cx43 expression may be crucial for the “leading front” formation during cancer invasion.
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spelling pubmed-62755692018-12-10 DU-145 prostate carcinoma cells that selectively transmigrate narrow obstacles express elevated levels of Cx43 Szpak, Katarzyna Wybieralska, Ewa Niedziałkowska, Ewa Rak, Monika Bechyne, Iga Michalik, Marta Madeja, Zbigniew Czyż, Jarosław Cell Mol Biol Lett Research Article The formation of aqueous intercellular channels mediating gap junctional intercellular coupling (GJIC) is a canonical function of connexins (Cx). In contrast, mechanisms of GJIC-independent involvement of connexins in cancer formation and metastasis remain a matter of debate. Because of the role of Cx43 in the determination of carcinoma cell invasive potential, we addressed the problem of the possible Cx43 involvement in early prostate cancer invasion. For this purpose, we analysed Cx43-positive DU-145 cell subsets established from the progenies of the cells most readily transmigrating microporous membranes. These progenies displayed motile activity similar to the control DU-145 cells but were characterized by elevated Cx43 expression levels and GJIC intensity. Thus, apparent links exist between Cx43 expression and transmigration potential of DU-145 cells. Moreover, Cx43 expression profiles in the analysed DU-145 subsets were not affected by intercellular contacts and chemical inhibition of GJIC during the transmigration. Our observations indicate that neither cell motility nor GJIC determines the transmigration efficiency of DU-145 cells. However, we postulate that selective transmigration of prostate cancer cells expressing elevated levels of Cx43 expression may be crucial for the “leading front” formation during cancer invasion. SP Versita 2011-09-12 /pmc/articles/PMC6275569/ /pubmed/21910090 http://dx.doi.org/10.2478/s11658-011-0027-7 Text en © © Versita Warsaw and Springer-Verlag Wien 2011
spellingShingle Research Article
Szpak, Katarzyna
Wybieralska, Ewa
Niedziałkowska, Ewa
Rak, Monika
Bechyne, Iga
Michalik, Marta
Madeja, Zbigniew
Czyż, Jarosław
DU-145 prostate carcinoma cells that selectively transmigrate narrow obstacles express elevated levels of Cx43
title DU-145 prostate carcinoma cells that selectively transmigrate narrow obstacles express elevated levels of Cx43
title_full DU-145 prostate carcinoma cells that selectively transmigrate narrow obstacles express elevated levels of Cx43
title_fullStr DU-145 prostate carcinoma cells that selectively transmigrate narrow obstacles express elevated levels of Cx43
title_full_unstemmed DU-145 prostate carcinoma cells that selectively transmigrate narrow obstacles express elevated levels of Cx43
title_short DU-145 prostate carcinoma cells that selectively transmigrate narrow obstacles express elevated levels of Cx43
title_sort du-145 prostate carcinoma cells that selectively transmigrate narrow obstacles express elevated levels of cx43
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275569/
https://www.ncbi.nlm.nih.gov/pubmed/21910090
http://dx.doi.org/10.2478/s11658-011-0027-7
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