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ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model

ZFAT, originally identified as a susceptibility gene for autoimmune thyroid disease, encodes a transcriptional regulator with one AT-hook and 18 C(2)H(2)-type zinc-finger domains. It is highly conserved among species. Here, we demonstrate that ZFAT is clearly expressed in human umbilical vein endoth...

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Autores principales: Yoshida, Yasuhiro, Tsunoda, Toshiyuki, Takashima, Yasuo, Fujimoto, Takahiro, Doi, Keiko, Sasazuki, Takehiko, Kuroki, Masahide, Iwasaki, Akinori, Shirasawa, Senji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SP Versita 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275617/
https://www.ncbi.nlm.nih.gov/pubmed/20645017
http://dx.doi.org/10.2478/s11658-010-0028-y
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author Yoshida, Yasuhiro
Tsunoda, Toshiyuki
Takashima, Yasuo
Fujimoto, Takahiro
Doi, Keiko
Sasazuki, Takehiko
Kuroki, Masahide
Iwasaki, Akinori
Shirasawa, Senji
author_facet Yoshida, Yasuhiro
Tsunoda, Toshiyuki
Takashima, Yasuo
Fujimoto, Takahiro
Doi, Keiko
Sasazuki, Takehiko
Kuroki, Masahide
Iwasaki, Akinori
Shirasawa, Senji
author_sort Yoshida, Yasuhiro
collection PubMed
description ZFAT, originally identified as a susceptibility gene for autoimmune thyroid disease, encodes a transcriptional regulator with one AT-hook and 18 C(2)H(2)-type zinc-finger domains. It is highly conserved among species. Here, we demonstrate that ZFAT is clearly expressed in human umbilical vein endothelial cells (HUVECs). Furthermore, we show that endothelial cell assembly and the branch point formation of capillary-like structures in HUVECs is impaired by the reduction of ZFAT expression through the use of ZFAT-miRNAs, whereas differences in cell proliferation or apoptotic features were not observed after the reduction in ZFAT expression. These results suggest that ZFAT may have critical roles in the capillary-like network formation that is involved in vascular remodeling. Elucidating the ZFAT-mediated transcriptional network will lead to a better understanding of the molecular mechanisms of angiogenesis.
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spelling pubmed-62756172018-12-10 ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model Yoshida, Yasuhiro Tsunoda, Toshiyuki Takashima, Yasuo Fujimoto, Takahiro Doi, Keiko Sasazuki, Takehiko Kuroki, Masahide Iwasaki, Akinori Shirasawa, Senji Cell Mol Biol Lett Short Communication ZFAT, originally identified as a susceptibility gene for autoimmune thyroid disease, encodes a transcriptional regulator with one AT-hook and 18 C(2)H(2)-type zinc-finger domains. It is highly conserved among species. Here, we demonstrate that ZFAT is clearly expressed in human umbilical vein endothelial cells (HUVECs). Furthermore, we show that endothelial cell assembly and the branch point formation of capillary-like structures in HUVECs is impaired by the reduction of ZFAT expression through the use of ZFAT-miRNAs, whereas differences in cell proliferation or apoptotic features were not observed after the reduction in ZFAT expression. These results suggest that ZFAT may have critical roles in the capillary-like network formation that is involved in vascular remodeling. Elucidating the ZFAT-mediated transcriptional network will lead to a better understanding of the molecular mechanisms of angiogenesis. SP Versita 2010-07-19 /pmc/articles/PMC6275617/ /pubmed/20645017 http://dx.doi.org/10.2478/s11658-010-0028-y Text en © © Versita Warsaw and Springer-Verlag Wien 2010
spellingShingle Short Communication
Yoshida, Yasuhiro
Tsunoda, Toshiyuki
Takashima, Yasuo
Fujimoto, Takahiro
Doi, Keiko
Sasazuki, Takehiko
Kuroki, Masahide
Iwasaki, Akinori
Shirasawa, Senji
ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model
title ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model
title_full ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model
title_fullStr ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model
title_full_unstemmed ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model
title_short ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model
title_sort zfat is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275617/
https://www.ncbi.nlm.nih.gov/pubmed/20645017
http://dx.doi.org/10.2478/s11658-010-0028-y
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