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Syntaxin 8 has two functionally distinct di-leucine-based motifs
Syntaxin 8 has been shown to form the SNARE complex with syntaxin 7, vti1b and endobrevin. These have been shown to function as the machinery for the homotypic fusion of late endosomes. Recently, we showed that syntaxins 7 and 8 cycle through the plasma membrane, and that the di-leucine-based motifs...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Versita
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275627/ https://www.ncbi.nlm.nih.gov/pubmed/17965969 http://dx.doi.org/10.2478/s11658-007-0043-9 |
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author | Kasai, Kazuo Suga, Kei Izumi, Tetsuro Akagawa, Kimio |
author_facet | Kasai, Kazuo Suga, Kei Izumi, Tetsuro Akagawa, Kimio |
author_sort | Kasai, Kazuo |
collection | PubMed |
description | Syntaxin 8 has been shown to form the SNARE complex with syntaxin 7, vti1b and endobrevin. These have been shown to function as the machinery for the homotypic fusion of late endosomes. Recently, we showed that syntaxins 7 and 8 cycle through the plasma membrane, and that the di-leucine-based motifs in the cytoplasmic domain of syntaxins 7 and 8 respectively function in their endocytic and exocytic processes. However, we could not elucidate the mechanism by which syntaxin 8 cycles through the plasma membrane. In this study, we constructed several different syntaxin 8 molecules by mutating putative di-leucine-based motifs, and analyzed their intracellular localization and trafficking. We found a di-leucine-based motif in the cytoplasmic domain of syntaxin 8. It is similar to that of syntaxin 7, and functions in its endocytosis. These results suggest that in the cytoplasmic domain, syntaxin 8 has two functionally distinct di-leucine-based motifs that act independently in its endocytic and exocytic processes. This is the first report on two di-leucine-based motifs in the same molecule acting independently in distinct transport pathways. |
format | Online Article Text |
id | pubmed-6275627 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Versita |
record_format | MEDLINE/PubMed |
spelling | pubmed-62756272018-12-10 Syntaxin 8 has two functionally distinct di-leucine-based motifs Kasai, Kazuo Suga, Kei Izumi, Tetsuro Akagawa, Kimio Cell Mol Biol Lett Research Article Syntaxin 8 has been shown to form the SNARE complex with syntaxin 7, vti1b and endobrevin. These have been shown to function as the machinery for the homotypic fusion of late endosomes. Recently, we showed that syntaxins 7 and 8 cycle through the plasma membrane, and that the di-leucine-based motifs in the cytoplasmic domain of syntaxins 7 and 8 respectively function in their endocytic and exocytic processes. However, we could not elucidate the mechanism by which syntaxin 8 cycles through the plasma membrane. In this study, we constructed several different syntaxin 8 molecules by mutating putative di-leucine-based motifs, and analyzed their intracellular localization and trafficking. We found a di-leucine-based motif in the cytoplasmic domain of syntaxin 8. It is similar to that of syntaxin 7, and functions in its endocytosis. These results suggest that in the cytoplasmic domain, syntaxin 8 has two functionally distinct di-leucine-based motifs that act independently in its endocytic and exocytic processes. This is the first report on two di-leucine-based motifs in the same molecule acting independently in distinct transport pathways. Versita 2007-10-29 /pmc/articles/PMC6275627/ /pubmed/17965969 http://dx.doi.org/10.2478/s11658-007-0043-9 Text en © University of Wrocław 2007 |
spellingShingle | Research Article Kasai, Kazuo Suga, Kei Izumi, Tetsuro Akagawa, Kimio Syntaxin 8 has two functionally distinct di-leucine-based motifs |
title | Syntaxin 8 has two functionally distinct di-leucine-based motifs |
title_full | Syntaxin 8 has two functionally distinct di-leucine-based motifs |
title_fullStr | Syntaxin 8 has two functionally distinct di-leucine-based motifs |
title_full_unstemmed | Syntaxin 8 has two functionally distinct di-leucine-based motifs |
title_short | Syntaxin 8 has two functionally distinct di-leucine-based motifs |
title_sort | syntaxin 8 has two functionally distinct di-leucine-based motifs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275627/ https://www.ncbi.nlm.nih.gov/pubmed/17965969 http://dx.doi.org/10.2478/s11658-007-0043-9 |
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