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Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations

Our study concerns the effects of exposure to lead chloride on the morphology, K(+) efflux, SO(4) (−) influx and GSH levels of the human erythrocyte. Blood was collected in heparinized tubes and washed three times. The cells were suspended at 3% hematocrit and incubated for 1 h at 25°C in a medium c...

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Autores principales: Gugliotta, Tiziana, De Luca, Grazia, Romano, Pietro, Rigano, Caterina, Scuteri, Adriana, Romano, Leonardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SP Versita 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275629/
https://www.ncbi.nlm.nih.gov/pubmed/22941203
http://dx.doi.org/10.2478/s11658-012-0027-2
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author Gugliotta, Tiziana
De Luca, Grazia
Romano, Pietro
Rigano, Caterina
Scuteri, Adriana
Romano, Leonardo
author_facet Gugliotta, Tiziana
De Luca, Grazia
Romano, Pietro
Rigano, Caterina
Scuteri, Adriana
Romano, Leonardo
author_sort Gugliotta, Tiziana
collection PubMed
description Our study concerns the effects of exposure to lead chloride on the morphology, K(+) efflux, SO(4) (−) influx and GSH levels of the human erythrocyte. Blood was collected in heparinized tubes and washed three times. The cells were suspended at 3% hematocrit and incubated for 1 h at 25°C in a medium containing increasing concentrations of lead chloride (0, 0.3, 0.5 and 1 μM). After incubation, the suspensions were centrifuged and the erythrocyte pellets were divided into three aliquots for testing. The results show: an increase in the permeability of erythrocytes treated with lead chloride with consequent damage and cellular death, especially in the presence of high concentrations; an increase in potassium ion efflux; alterations in the morphology and membrane structure of the red blood cells; and a decrease in sulphate uptake, due either to the oxidative effect of this compound on the band 3 protein, which loses its biological valence as a carrier of sulphate ions, or to a decrease in the ATP erythrocyte concentration. In conclusion, the exposure of erythrocytes to Pb(2+) ions leads to a reduction in the average lifetime of the erythrocytes and the subsequent development of anemia. These data are discussed in terms of the possible effect of lead on the reduction-oxidation systems of the cell. Oxidant agents, such as lead, are known to cross-link integral membrane proteins, leading to K/Cl-cotransport. The increased K(+) efflux affects the altered redox state.
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spelling pubmed-62756292018-12-10 Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations Gugliotta, Tiziana De Luca, Grazia Romano, Pietro Rigano, Caterina Scuteri, Adriana Romano, Leonardo Cell Mol Biol Lett Research Article Our study concerns the effects of exposure to lead chloride on the morphology, K(+) efflux, SO(4) (−) influx and GSH levels of the human erythrocyte. Blood was collected in heparinized tubes and washed three times. The cells were suspended at 3% hematocrit and incubated for 1 h at 25°C in a medium containing increasing concentrations of lead chloride (0, 0.3, 0.5 and 1 μM). After incubation, the suspensions were centrifuged and the erythrocyte pellets were divided into three aliquots for testing. The results show: an increase in the permeability of erythrocytes treated with lead chloride with consequent damage and cellular death, especially in the presence of high concentrations; an increase in potassium ion efflux; alterations in the morphology and membrane structure of the red blood cells; and a decrease in sulphate uptake, due either to the oxidative effect of this compound on the band 3 protein, which loses its biological valence as a carrier of sulphate ions, or to a decrease in the ATP erythrocyte concentration. In conclusion, the exposure of erythrocytes to Pb(2+) ions leads to a reduction in the average lifetime of the erythrocytes and the subsequent development of anemia. These data are discussed in terms of the possible effect of lead on the reduction-oxidation systems of the cell. Oxidant agents, such as lead, are known to cross-link integral membrane proteins, leading to K/Cl-cotransport. The increased K(+) efflux affects the altered redox state. SP Versita 2012-09-01 /pmc/articles/PMC6275629/ /pubmed/22941203 http://dx.doi.org/10.2478/s11658-012-0027-2 Text en © Versita Warsaw and Springer-Verlag Wien 2012
spellingShingle Research Article
Gugliotta, Tiziana
De Luca, Grazia
Romano, Pietro
Rigano, Caterina
Scuteri, Adriana
Romano, Leonardo
Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations
title Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations
title_full Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations
title_fullStr Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations
title_full_unstemmed Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations
title_short Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations
title_sort effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275629/
https://www.ncbi.nlm.nih.gov/pubmed/22941203
http://dx.doi.org/10.2478/s11658-012-0027-2
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