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The role of Alk-1 and Alk-5 in the mechanosensing of chondrocytes

We aim to demonstrate the role of Alk receptors in the response of hydrogel expansion. Chondrocytes from rat knees were cultured onto plastic and hydrogel surfaces. Alk-1 and Alk-5 were overexpressed or silenced and the effects on cells during expansion were tested and confirmed using peptide inhibi...

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Detalles Bibliográficos
Autores principales: Sanz-Ramos, Patricia, Dotor, Javier, Izal-Azcárate, Iñigo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Versita 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275650/
https://www.ncbi.nlm.nih.gov/pubmed/25424912
http://dx.doi.org/10.2478/s11658-014-0220-6
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author Sanz-Ramos, Patricia
Dotor, Javier
Izal-Azcárate, Iñigo
author_facet Sanz-Ramos, Patricia
Dotor, Javier
Izal-Azcárate, Iñigo
author_sort Sanz-Ramos, Patricia
collection PubMed
description We aim to demonstrate the role of Alk receptors in the response of hydrogel expansion. Chondrocytes from rat knees were cultured onto plastic and hydrogel surfaces. Alk-1 and Alk-5 were overexpressed or silenced and the effects on cells during expansion were tested and confirmed using peptide inhibitors for TGFβ. Overexpression of Alk-5 and silencing of Alk-1 led to a loss of the chondrocyte phenotype, proving that they are key regulators of chondrocyte mechanosensing. An analysis of the gene expression profile during the expansion of these modified cartilage cells in plastic showed a better maintenance of the chondrocyte phenotype, at least during the first passages. These passages were also assayed in a mouse model of intramuscular chondrogenesis. Our findings indicate that these two receptors are important mediators in the response of chondrocytes to changes in the mechanical environment, making them suitable targets for modulating chondrogenesis. Inhibition of TGFβ could also be effective in improving chondrocyte activity in aged or expanded cells that overexpress Alk-1.
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spelling pubmed-62756502018-12-10 The role of Alk-1 and Alk-5 in the mechanosensing of chondrocytes Sanz-Ramos, Patricia Dotor, Javier Izal-Azcárate, Iñigo Cell Mol Biol Lett Research Article We aim to demonstrate the role of Alk receptors in the response of hydrogel expansion. Chondrocytes from rat knees were cultured onto plastic and hydrogel surfaces. Alk-1 and Alk-5 were overexpressed or silenced and the effects on cells during expansion were tested and confirmed using peptide inhibitors for TGFβ. Overexpression of Alk-5 and silencing of Alk-1 led to a loss of the chondrocyte phenotype, proving that they are key regulators of chondrocyte mechanosensing. An analysis of the gene expression profile during the expansion of these modified cartilage cells in plastic showed a better maintenance of the chondrocyte phenotype, at least during the first passages. These passages were also assayed in a mouse model of intramuscular chondrogenesis. Our findings indicate that these two receptors are important mediators in the response of chondrocytes to changes in the mechanical environment, making them suitable targets for modulating chondrogenesis. Inhibition of TGFβ could also be effective in improving chondrocyte activity in aged or expanded cells that overexpress Alk-1. Versita 2014-11-25 /pmc/articles/PMC6275650/ /pubmed/25424912 http://dx.doi.org/10.2478/s11658-014-0220-6 Text en © Versita Warsaw and Springer-Verlag Wien 2014
spellingShingle Research Article
Sanz-Ramos, Patricia
Dotor, Javier
Izal-Azcárate, Iñigo
The role of Alk-1 and Alk-5 in the mechanosensing of chondrocytes
title The role of Alk-1 and Alk-5 in the mechanosensing of chondrocytes
title_full The role of Alk-1 and Alk-5 in the mechanosensing of chondrocytes
title_fullStr The role of Alk-1 and Alk-5 in the mechanosensing of chondrocytes
title_full_unstemmed The role of Alk-1 and Alk-5 in the mechanosensing of chondrocytes
title_short The role of Alk-1 and Alk-5 in the mechanosensing of chondrocytes
title_sort role of alk-1 and alk-5 in the mechanosensing of chondrocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275650/
https://www.ncbi.nlm.nih.gov/pubmed/25424912
http://dx.doi.org/10.2478/s11658-014-0220-6
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