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The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea

Endothelin (ET), originally characterized as a vasoconstrictive peptide, has been found to have many different biological functions, including acting as a local hormonal regulator of pressure, fluid, ions and neurotransmitters in the inner ear. The objective of this study was to examine and quantify...

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Autores principales: Luo, Yan, Tang, Yuedi, Xia, Qingjie, Liu, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Versita 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275685/
https://www.ncbi.nlm.nih.gov/pubmed/17607529
http://dx.doi.org/10.2478/s11658-007-0027-9
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author Luo, Yan
Tang, Yuedi
Xia, Qingjie
Liu, Jin
author_facet Luo, Yan
Tang, Yuedi
Xia, Qingjie
Liu, Jin
author_sort Luo, Yan
collection PubMed
description Endothelin (ET), originally characterized as a vasoconstrictive peptide, has been found to have many different biological functions, including acting as a local hormonal regulator of pressure, fluid, ions and neurotransmitters in the inner ear. The objective of this study was to examine and quantify the mRNA expression of the endothelin type A and B receptors (ETAR and ETBR) in the strial vascularies (StV) and non-strial tissues (NSt) of the cochlear lateral wall using the real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) technique. The mouse tissue samples were harvested and RNA was extracted. RT was performed to obtain cDNA, and then the mRNA expression of each gene was measured via real-time PCR. We found that both receptor subtypes were expressed in the cochlear lateral wall, with a predominance of ETAR over ETBR. We showed that the mRNA expression of the two receptor subtypes was higher in the StV with a 1.8 times higher level of ETAR and an 8.1 times higher level of ETBR mRNAs than in the adjacent NSt of the lateral wall tissue. This study shows the existence and the quantity of ET receptor subtypes in the StV and NSt of the mouse cochlea. Our results suggest that an endothelin-mediated response via two different receptors, ETAR and ETBR, may play an important role in the physiological functions of the cochlear lateral wall by maintaining the homeostatic environment of the cochlea.
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spelling pubmed-62756852018-12-10 The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea Luo, Yan Tang, Yuedi Xia, Qingjie Liu, Jin Cell Mol Biol Lett Short Communication Endothelin (ET), originally characterized as a vasoconstrictive peptide, has been found to have many different biological functions, including acting as a local hormonal regulator of pressure, fluid, ions and neurotransmitters in the inner ear. The objective of this study was to examine and quantify the mRNA expression of the endothelin type A and B receptors (ETAR and ETBR) in the strial vascularies (StV) and non-strial tissues (NSt) of the cochlear lateral wall using the real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) technique. The mouse tissue samples were harvested and RNA was extracted. RT was performed to obtain cDNA, and then the mRNA expression of each gene was measured via real-time PCR. We found that both receptor subtypes were expressed in the cochlear lateral wall, with a predominance of ETAR over ETBR. We showed that the mRNA expression of the two receptor subtypes was higher in the StV with a 1.8 times higher level of ETAR and an 8.1 times higher level of ETBR mRNAs than in the adjacent NSt of the lateral wall tissue. This study shows the existence and the quantity of ET receptor subtypes in the StV and NSt of the mouse cochlea. Our results suggest that an endothelin-mediated response via two different receptors, ETAR and ETBR, may play an important role in the physiological functions of the cochlear lateral wall by maintaining the homeostatic environment of the cochlea. Versita 2007-07-03 /pmc/articles/PMC6275685/ /pubmed/17607529 http://dx.doi.org/10.2478/s11658-007-0027-9 Text en © University of Wrocław 2007
spellingShingle Short Communication
Luo, Yan
Tang, Yuedi
Xia, Qingjie
Liu, Jin
The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea
title The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea
title_full The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea
title_fullStr The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea
title_full_unstemmed The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea
title_short The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea
title_sort expression of endothelin type a and b receptors in the lateral wall of the mouse cochlea
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275685/
https://www.ncbi.nlm.nih.gov/pubmed/17607529
http://dx.doi.org/10.2478/s11658-007-0027-9
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