Identification of microsatellite markers in the rye genome

The rye genomic library, which consists of DNA fragments in the range of 0.5–1.1 kb, was screened for the presence of tri-and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pa...

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Detalles Bibliográficos
Autores principales: Bolibok, Hanna, Rakoczy-Trojanowska, Monika, Wyrzykowska, Małgorzata, Radecka, Magdalena, Orczyk, Wacław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Versita 2006
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275887/
https://www.ncbi.nlm.nih.gov/pubmed/16847573
http://dx.doi.org/10.2478/s11658-006-0023-5
Descripción
Sumario:The rye genomic library, which consists of DNA fragments in the range of 0.5–1.1 kb, was screened for the presence of tri-and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pairs amplified specific products, and six of them were capable of detecting polymorphism among 30 rye accessions of different genetic backgrounds. Using a set of Chinese Spring-Imperial wheat-rye addition lines, it was possible to locate 3 newly identified microsatellites on chromosomes 3R, 4R and 7R.

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