Identification of microsatellite markers in the rye genome

The rye genomic library, which consists of DNA fragments in the range of 0.5–1.1 kb, was screened for the presence of tri-and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pa...

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Detalles Bibliográficos
Autores principales: Bolibok, Hanna, Rakoczy-Trojanowska, Monika, Wyrzykowska, Małgorzata, Radecka, Magdalena, Orczyk, Wacław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Versita 2006
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275887/
https://www.ncbi.nlm.nih.gov/pubmed/16847573
http://dx.doi.org/10.2478/s11658-006-0023-5
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author Bolibok, Hanna
Rakoczy-Trojanowska, Monika
Wyrzykowska, Małgorzata
Radecka, Magdalena
Orczyk, Wacław
author_facet Bolibok, Hanna
Rakoczy-Trojanowska, Monika
Wyrzykowska, Małgorzata
Radecka, Magdalena
Orczyk, Wacław
author_sort Bolibok, Hanna
collection PubMed
description The rye genomic library, which consists of DNA fragments in the range of 0.5–1.1 kb, was screened for the presence of tri-and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pairs amplified specific products, and six of them were capable of detecting polymorphism among 30 rye accessions of different genetic backgrounds. Using a set of Chinese Spring-Imperial wheat-rye addition lines, it was possible to locate 3 newly identified microsatellites on chromosomes 3R, 4R and 7R.
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spelling pubmed-62758872018-12-10 Identification of microsatellite markers in the rye genome Bolibok, Hanna Rakoczy-Trojanowska, Monika Wyrzykowska, Małgorzata Radecka, Magdalena Orczyk, Wacław Cell Mol Biol Lett Short Communication The rye genomic library, which consists of DNA fragments in the range of 0.5–1.1 kb, was screened for the presence of tri-and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pairs amplified specific products, and six of them were capable of detecting polymorphism among 30 rye accessions of different genetic backgrounds. Using a set of Chinese Spring-Imperial wheat-rye addition lines, it was possible to locate 3 newly identified microsatellites on chromosomes 3R, 4R and 7R. Versita 2006-06-01 /pmc/articles/PMC6275887/ /pubmed/16847573 http://dx.doi.org/10.2478/s11658-006-0023-5 Text en © University of Wrocław 2006
spellingShingle Short Communication
Bolibok, Hanna
Rakoczy-Trojanowska, Monika
Wyrzykowska, Małgorzata
Radecka, Magdalena
Orczyk, Wacław
Identification of microsatellite markers in the rye genome
title Identification of microsatellite markers in the rye genome
title_full Identification of microsatellite markers in the rye genome
title_fullStr Identification of microsatellite markers in the rye genome
title_full_unstemmed Identification of microsatellite markers in the rye genome
title_short Identification of microsatellite markers in the rye genome
title_sort identification of microsatellite markers in the rye genome
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275887/
https://www.ncbi.nlm.nih.gov/pubmed/16847573
http://dx.doi.org/10.2478/s11658-006-0023-5
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