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Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells
The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the prolif...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SP Versita
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275915/ https://www.ncbi.nlm.nih.gov/pubmed/21161417 http://dx.doi.org/10.2478/s11658-010-0039-8 |
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author | Anisimov, Sergey V. Christophersen, Nicolaj S. Correia, Ana S. Hall, Vanessa J. Sandelin, Ingrid Li, Jia-Yi Brundin, Patrik |
author_facet | Anisimov, Sergey V. Christophersen, Nicolaj S. Correia, Ana S. Hall, Vanessa J. Sandelin, Ingrid Li, Jia-Yi Brundin, Patrik |
author_sort | Anisimov, Sergey V. |
collection | PubMed |
description | The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates. ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available for this article at 10.2478/s11658-010-0039-8 and is accessible for authorized users. |
format | Online Article Text |
id | pubmed-6275915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | SP Versita |
record_format | MEDLINE/PubMed |
spelling | pubmed-62759152018-12-10 Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells Anisimov, Sergey V. Christophersen, Nicolaj S. Correia, Ana S. Hall, Vanessa J. Sandelin, Ingrid Li, Jia-Yi Brundin, Patrik Cell Mol Biol Lett Short Communication The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates. ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available for this article at 10.2478/s11658-010-0039-8 and is accessible for authorized users. SP Versita 2010-12-15 /pmc/articles/PMC6275915/ /pubmed/21161417 http://dx.doi.org/10.2478/s11658-010-0039-8 Text en © © Versita Warsaw and Springer-Verlag Wien 2011 |
spellingShingle | Short Communication Anisimov, Sergey V. Christophersen, Nicolaj S. Correia, Ana S. Hall, Vanessa J. Sandelin, Ingrid Li, Jia-Yi Brundin, Patrik Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells |
title | Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells |
title_full | Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells |
title_fullStr | Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells |
title_full_unstemmed | Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells |
title_short | Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells |
title_sort | identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275915/ https://www.ncbi.nlm.nih.gov/pubmed/21161417 http://dx.doi.org/10.2478/s11658-010-0039-8 |
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