The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100

Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We ass...

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Detalles Bibliográficos
Autores principales: Panasiewicz, Mirosława, Domek, Hanna, Hoser, Grażyna, Fedoryszak, Natalia, Kawalec, Maciej, Pacuszka, Tadeusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SP Versita 2008
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275918/
https://www.ncbi.nlm.nih.gov/pubmed/18979068
http://dx.doi.org/10.2478/s11658-008-0043-4
Descripción
Sumario:Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [(3)H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C(14), C(18) or C(18:1) fatty acids (Fas)

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