The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100

Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We ass...

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Detalles Bibliográficos
Autores principales: Panasiewicz, Mirosława, Domek, Hanna, Hoser, Grażyna, Fedoryszak, Natalia, Kawalec, Maciej, Pacuszka, Tadeusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SP Versita 2008
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275918/
https://www.ncbi.nlm.nih.gov/pubmed/18979068
http://dx.doi.org/10.2478/s11658-008-0043-4
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author Panasiewicz, Mirosława
Domek, Hanna
Hoser, Grażyna
Fedoryszak, Natalia
Kawalec, Maciej
Pacuszka, Tadeusz
author_facet Panasiewicz, Mirosława
Domek, Hanna
Hoser, Grażyna
Fedoryszak, Natalia
Kawalec, Maciej
Pacuszka, Tadeusz
author_sort Panasiewicz, Mirosława
collection PubMed
description Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [(3)H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C(14), C(18) or C(18:1) fatty acids (Fas)
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spelling pubmed-62759182018-12-10 The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100 Panasiewicz, Mirosława Domek, Hanna Hoser, Grażyna Fedoryszak, Natalia Kawalec, Maciej Pacuszka, Tadeusz Cell Mol Biol Lett Short Communication Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [(3)H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C(14), C(18) or C(18:1) fatty acids (Fas) SP Versita 2008-10-31 /pmc/articles/PMC6275918/ /pubmed/18979068 http://dx.doi.org/10.2478/s11658-008-0043-4 Text en © © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2008
spellingShingle Short Communication
Panasiewicz, Mirosława
Domek, Hanna
Hoser, Grażyna
Fedoryszak, Natalia
Kawalec, Maciej
Pacuszka, Tadeusz
The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100
title The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100
title_full The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100
title_fullStr The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100
title_full_unstemmed The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100
title_short The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100
title_sort ceramide structure of gm1 ganglioside differently affects its recovery in low-density membrane fractions prepared from hl-60 cells with or without triton-x100
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275918/
https://www.ncbi.nlm.nih.gov/pubmed/18979068
http://dx.doi.org/10.2478/s11658-008-0043-4
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