Improved Synthesis of 2-Trifluoromethyl-10-aminopropylphenothiazine: Making 2-Trifluoromethyl-10-aminopropylphenothiazine Readily Available for Calmodulin Purification

[Image: see text] An improved and high yielding three-step synthesis for the production of 2-trifluoromethyl-10-aminopropylphenothiazine (TAPP) using less hazardous and more inexpensive reagents, its coupling to Sepharose-4B resin, and its ability to purify calmodulin are described. The overall yiel...

Descripción completa

Detalles Bibliográficos
Autores principales: Johnson, Josiah W., Cain, Kyle W., Dunlap, Tori B., Naumiec, Gregory R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275952/
https://www.ncbi.nlm.nih.gov/pubmed/30533586
http://dx.doi.org/10.1021/acsomega.8b02146
_version_ 1783377916239806464
author Johnson, Josiah W.
Cain, Kyle W.
Dunlap, Tori B.
Naumiec, Gregory R.
author_facet Johnson, Josiah W.
Cain, Kyle W.
Dunlap, Tori B.
Naumiec, Gregory R.
author_sort Johnson, Josiah W.
collection PubMed
description [Image: see text] An improved and high yielding three-step synthesis for the production of 2-trifluoromethyl-10-aminopropylphenothiazine (TAPP) using less hazardous and more inexpensive reagents, its coupling to Sepharose-4B resin, and its ability to purify calmodulin are described. The overall yield of TAPP, starting with 3-aminopropyl bromide hydrobromide and 2-(trifluoromethyl)phenothiazine, was 96%.
format Online
Article
Text
id pubmed-6275952
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-62759522018-12-05 Improved Synthesis of 2-Trifluoromethyl-10-aminopropylphenothiazine: Making 2-Trifluoromethyl-10-aminopropylphenothiazine Readily Available for Calmodulin Purification Johnson, Josiah W. Cain, Kyle W. Dunlap, Tori B. Naumiec, Gregory R. ACS Omega [Image: see text] An improved and high yielding three-step synthesis for the production of 2-trifluoromethyl-10-aminopropylphenothiazine (TAPP) using less hazardous and more inexpensive reagents, its coupling to Sepharose-4B resin, and its ability to purify calmodulin are described. The overall yield of TAPP, starting with 3-aminopropyl bromide hydrobromide and 2-(trifluoromethyl)phenothiazine, was 96%. American Chemical Society 2018-11-30 /pmc/articles/PMC6275952/ /pubmed/30533586 http://dx.doi.org/10.1021/acsomega.8b02146 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Johnson, Josiah W.
Cain, Kyle W.
Dunlap, Tori B.
Naumiec, Gregory R.
Improved Synthesis of 2-Trifluoromethyl-10-aminopropylphenothiazine: Making 2-Trifluoromethyl-10-aminopropylphenothiazine Readily Available for Calmodulin Purification
title Improved Synthesis of 2-Trifluoromethyl-10-aminopropylphenothiazine: Making 2-Trifluoromethyl-10-aminopropylphenothiazine Readily Available for Calmodulin Purification
title_full Improved Synthesis of 2-Trifluoromethyl-10-aminopropylphenothiazine: Making 2-Trifluoromethyl-10-aminopropylphenothiazine Readily Available for Calmodulin Purification
title_fullStr Improved Synthesis of 2-Trifluoromethyl-10-aminopropylphenothiazine: Making 2-Trifluoromethyl-10-aminopropylphenothiazine Readily Available for Calmodulin Purification
title_full_unstemmed Improved Synthesis of 2-Trifluoromethyl-10-aminopropylphenothiazine: Making 2-Trifluoromethyl-10-aminopropylphenothiazine Readily Available for Calmodulin Purification
title_short Improved Synthesis of 2-Trifluoromethyl-10-aminopropylphenothiazine: Making 2-Trifluoromethyl-10-aminopropylphenothiazine Readily Available for Calmodulin Purification
title_sort improved synthesis of 2-trifluoromethyl-10-aminopropylphenothiazine: making 2-trifluoromethyl-10-aminopropylphenothiazine readily available for calmodulin purification
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275952/
https://www.ncbi.nlm.nih.gov/pubmed/30533586
http://dx.doi.org/10.1021/acsomega.8b02146
work_keys_str_mv AT johnsonjosiahw improvedsynthesisof2trifluoromethyl10aminopropylphenothiazinemaking2trifluoromethyl10aminopropylphenothiazinereadilyavailableforcalmodulinpurification
AT cainkylew improvedsynthesisof2trifluoromethyl10aminopropylphenothiazinemaking2trifluoromethyl10aminopropylphenothiazinereadilyavailableforcalmodulinpurification
AT dunlaptorib improvedsynthesisof2trifluoromethyl10aminopropylphenothiazinemaking2trifluoromethyl10aminopropylphenothiazinereadilyavailableforcalmodulinpurification
AT naumiecgregoryr improvedsynthesisof2trifluoromethyl10aminopropylphenothiazinemaking2trifluoromethyl10aminopropylphenothiazinereadilyavailableforcalmodulinpurification

Ejemplares similares