The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’

Cloning using bacterial artificial chromosomes (BACs) can yield high quality genomic libraries, which are used for the physical mapping, identification and isolation of genes, and for gene sequencing. A BAC genomic library was constructed from high molecular weight DNA (HMW DNA) obtained from nuclei...

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Autores principales: Gutman, Wojciech, Pawełkowicz, Magdalena, Woycicki, Rafał, Piszczek, Ewa, Przybecki, Zbigniew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Versita 2007
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275958/
https://www.ncbi.nlm.nih.gov/pubmed/17965974
http://dx.doi.org/10.2478/s11658-007-0038-6
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author Gutman, Wojciech
Pawełkowicz, Magdalena
Woycicki, Rafał
Piszczek, Ewa
Przybecki, Zbigniew
author_facet Gutman, Wojciech
Pawełkowicz, Magdalena
Woycicki, Rafał
Piszczek, Ewa
Przybecki, Zbigniew
author_sort Gutman, Wojciech
collection PubMed
description Cloning using bacterial artificial chromosomes (BACs) can yield high quality genomic libraries, which are used for the physical mapping, identification and isolation of genes, and for gene sequencing. A BAC genomic library was constructed from high molecular weight DNA (HMW DNA) obtained from nuclei of the cucumber (Cucumis sativus L. cv. Borszczagowski; B10 line). The DNA was digested with the HindIII restriction enzyme and ligated into the pCC1BAC vector. The library consists of 34,560 BAC clones with an average insert size of 135 kb, and 12.7x genome coverage. Screening the library for chloroplast and mitochondrial DNA content indicated an exceptionally low 0.26% contamination with chloroplast DNA and 0.3% with mitochondrial DNA.
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spelling pubmed-62759582018-12-10 The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’ Gutman, Wojciech Pawełkowicz, Magdalena Woycicki, Rafał Piszczek, Ewa Przybecki, Zbigniew Cell Mol Biol Lett Research Article Cloning using bacterial artificial chromosomes (BACs) can yield high quality genomic libraries, which are used for the physical mapping, identification and isolation of genes, and for gene sequencing. A BAC genomic library was constructed from high molecular weight DNA (HMW DNA) obtained from nuclei of the cucumber (Cucumis sativus L. cv. Borszczagowski; B10 line). The DNA was digested with the HindIII restriction enzyme and ligated into the pCC1BAC vector. The library consists of 34,560 BAC clones with an average insert size of 135 kb, and 12.7x genome coverage. Screening the library for chloroplast and mitochondrial DNA content indicated an exceptionally low 0.26% contamination with chloroplast DNA and 0.3% with mitochondrial DNA. Versita 2007-10-29 /pmc/articles/PMC6275958/ /pubmed/17965974 http://dx.doi.org/10.2478/s11658-007-0038-6 Text en © University of Wrocław 2007
spellingShingle Research Article
Gutman, Wojciech
Pawełkowicz, Magdalena
Woycicki, Rafał
Piszczek, Ewa
Przybecki, Zbigniew
The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’
title The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’
title_full The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’
title_fullStr The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’
title_full_unstemmed The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’
title_short The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’
title_sort construction and characteristics of a bac library for cucumis sativus l. ‘b10’
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275958/
https://www.ncbi.nlm.nih.gov/pubmed/17965974
http://dx.doi.org/10.2478/s11658-007-0038-6
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