MiR-34a regulates apoptosis in liver cells by targeting the KLF4 gene

MicroRNAs (miRNAs) regulate gene expression by inhibiting translation or targeting messenger RNA (mRNA) for degradation in a posttranscriptional fashion. In this study, we show that ectopic expression of miR-34a-5p reduces the mRNA and protein levels of Krüppel-like factor 4 (KLF4). We also demonstr...

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Detalles Bibliográficos
Autores principales: Chen, Qiu, Li, Lei, Tu, Yu, Zheng, Lu Lin, Liu, Wei, Zuo, Xue Yong, He, Yong Ming, Zhang, Shu Yu, Zhu, Wei, Cao, Jian Ping, Cui, Feng Mei, Hou, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Versita 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275989/
https://www.ncbi.nlm.nih.gov/pubmed/24415058
http://dx.doi.org/10.2478/s11658-013-0115-y
Descripción
Sumario:MicroRNAs (miRNAs) regulate gene expression by inhibiting translation or targeting messenger RNA (mRNA) for degradation in a posttranscriptional fashion. In this study, we show that ectopic expression of miR-34a-5p reduces the mRNA and protein levels of Krüppel-like factor 4 (KLF4). We also demonstrate that miR-34a targets the 3′-untranslated mRNA region of KLF4 and show that overexpression of miR-34a induces a significant level of apoptosis in BNL CL.2 cells exposed to doxorubicin or 10 Gy X-ray. Our data suggest that the effects of miR-34a on apoptosis occur due to the downregulation of KLF4.

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