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Maize male sterile 33 encodes a putative glycerol-3-phosphate acyltransferase that mediates anther cuticle formation and microspore development

BACKGROUND: The anther cuticle, which is primarily composed of lipid polymers, is crucial for pollen development and plays important roles in sexual reproduction in higher plants. However, the mechanism underlying the biosynthesis of lipid polymers in maize (Zea mays. L.) remains unclear. RESULTS: H...

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Autores principales: Zhang, Lei, Luo, Hongbing, Zhao, Yue, Chen, Xiaoyang, Huang, Yumin, Yan, Shuangshuang, Li, Suxing, Liu, Meishan, Huang, Wei, Zhang, Xiaolan, Jin, Weiwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6276174/
https://www.ncbi.nlm.nih.gov/pubmed/30509161
http://dx.doi.org/10.1186/s12870-018-1543-7
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author Zhang, Lei
Luo, Hongbing
Zhao, Yue
Chen, Xiaoyang
Huang, Yumin
Yan, Shuangshuang
Li, Suxing
Liu, Meishan
Huang, Wei
Zhang, Xiaolan
Jin, Weiwei
author_facet Zhang, Lei
Luo, Hongbing
Zhao, Yue
Chen, Xiaoyang
Huang, Yumin
Yan, Shuangshuang
Li, Suxing
Liu, Meishan
Huang, Wei
Zhang, Xiaolan
Jin, Weiwei
author_sort Zhang, Lei
collection PubMed
description BACKGROUND: The anther cuticle, which is primarily composed of lipid polymers, is crucial for pollen development and plays important roles in sexual reproduction in higher plants. However, the mechanism underlying the biosynthesis of lipid polymers in maize (Zea mays. L.) remains unclear. RESULTS: Here, we report that the maize male-sterile mutant shrinking anther 1 (sa1), which is allelic to the classic mutant male sterile 33 (ms33), displays defective anther cuticle development and premature microspore degradation. We isolated MS33 via map-based cloning. MS33 encodes a putative glycerol-3-phosphate acyltransferase and is preferentially expressed in tapetal cells during anther development. Gas chromatography-mass spectrometry revealed a substantial reduction in wax and cutin in ms33 anthers compared to wild type. Accordingly, RNA-sequencing analysis showed that many genes involved in wax and cutin biosynthesis are differentially expressed in ms33 compared to wild type. CONCLUSIONS: Our findings suggest that MS33 may contribute to anther cuticle and microspore development by affecting lipid polyester biosynthesis in maize. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1543-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-62761742018-12-06 Maize male sterile 33 encodes a putative glycerol-3-phosphate acyltransferase that mediates anther cuticle formation and microspore development Zhang, Lei Luo, Hongbing Zhao, Yue Chen, Xiaoyang Huang, Yumin Yan, Shuangshuang Li, Suxing Liu, Meishan Huang, Wei Zhang, Xiaolan Jin, Weiwei BMC Plant Biol Research Article BACKGROUND: The anther cuticle, which is primarily composed of lipid polymers, is crucial for pollen development and plays important roles in sexual reproduction in higher plants. However, the mechanism underlying the biosynthesis of lipid polymers in maize (Zea mays. L.) remains unclear. RESULTS: Here, we report that the maize male-sterile mutant shrinking anther 1 (sa1), which is allelic to the classic mutant male sterile 33 (ms33), displays defective anther cuticle development and premature microspore degradation. We isolated MS33 via map-based cloning. MS33 encodes a putative glycerol-3-phosphate acyltransferase and is preferentially expressed in tapetal cells during anther development. Gas chromatography-mass spectrometry revealed a substantial reduction in wax and cutin in ms33 anthers compared to wild type. Accordingly, RNA-sequencing analysis showed that many genes involved in wax and cutin biosynthesis are differentially expressed in ms33 compared to wild type. CONCLUSIONS: Our findings suggest that MS33 may contribute to anther cuticle and microspore development by affecting lipid polyester biosynthesis in maize. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1543-7) contains supplementary material, which is available to authorized users. BioMed Central 2018-12-03 /pmc/articles/PMC6276174/ /pubmed/30509161 http://dx.doi.org/10.1186/s12870-018-1543-7 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Lei
Luo, Hongbing
Zhao, Yue
Chen, Xiaoyang
Huang, Yumin
Yan, Shuangshuang
Li, Suxing
Liu, Meishan
Huang, Wei
Zhang, Xiaolan
Jin, Weiwei
Maize male sterile 33 encodes a putative glycerol-3-phosphate acyltransferase that mediates anther cuticle formation and microspore development
title Maize male sterile 33 encodes a putative glycerol-3-phosphate acyltransferase that mediates anther cuticle formation and microspore development
title_full Maize male sterile 33 encodes a putative glycerol-3-phosphate acyltransferase that mediates anther cuticle formation and microspore development
title_fullStr Maize male sterile 33 encodes a putative glycerol-3-phosphate acyltransferase that mediates anther cuticle formation and microspore development
title_full_unstemmed Maize male sterile 33 encodes a putative glycerol-3-phosphate acyltransferase that mediates anther cuticle formation and microspore development
title_short Maize male sterile 33 encodes a putative glycerol-3-phosphate acyltransferase that mediates anther cuticle formation and microspore development
title_sort maize male sterile 33 encodes a putative glycerol-3-phosphate acyltransferase that mediates anther cuticle formation and microspore development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6276174/
https://www.ncbi.nlm.nih.gov/pubmed/30509161
http://dx.doi.org/10.1186/s12870-018-1543-7
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