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Cereal aphids differently affect benzoxazinoid levels in durum wheat

Aphids are major pests in cereal crops that cause direct and indirect damage leading to yield reduction. Despite the fact that wheat provides 20% of the world’s caloric and protein diet, its metabolic responses to aphid attack, in general, and specifically its production of benzoxazinoid defense com...

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Autores principales: Shavit, Reut, Batyrshina, Zhaniya S., Dotan, Nitsan, Tzin, Vered
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277073/
https://www.ncbi.nlm.nih.gov/pubmed/30507950
http://dx.doi.org/10.1371/journal.pone.0208103
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author Shavit, Reut
Batyrshina, Zhaniya S.
Dotan, Nitsan
Tzin, Vered
author_facet Shavit, Reut
Batyrshina, Zhaniya S.
Dotan, Nitsan
Tzin, Vered
author_sort Shavit, Reut
collection PubMed
description Aphids are major pests in cereal crops that cause direct and indirect damage leading to yield reduction. Despite the fact that wheat provides 20% of the world’s caloric and protein diet, its metabolic responses to aphid attack, in general, and specifically its production of benzoxazinoid defense compounds are poorly understood. The objective of this study was to compare the metabolic diversity of durum wheat seedlings (Triticum turgidum ssp. durum) under attack by three different cereal aphids: i) the English grain aphid (Sitobion avenae Fabricius), ii) the bird cherry-oat aphid (Rhopalosiphum padi L.), and iii) the greenbug aphid (Schizaphis graminum Rondani), which are some of the most destructive aphid species to wheat. Insect progeny bioassays and metabolic analyses using chromatography/Q-Exactive/mass spectrometry non-targeted metabolomics and a targeted benzoxazinoid profile were performed on infested leaves. The insect bioassays revealed that the plants were susceptible to S. graminum, resistant to S. avenae, and mildly resistant to R. padi. The metabolic analyses of benzoxazinoids suggested that the predominant metabolites DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin- 3-one) and its glycosylated form DIMBOA-glucoside (Glc) were significantly induced upon both S. avenae, and R. padi aphid feeding. However, the levels of the benzoxazinoid metabolite HDMBOA-Glc (2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside) were enhanced due to the feeding of S. avenae and S. graminum aphids, to which Svevo was the most resistant and the most susceptible, respectively. The results showed a partial correlation between the induction of benzoxazinoids and aphid reproduction. Overall, our observations revealed diverse metabolic responses of wheat seedlings to cereal aphid feeding.
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spelling pubmed-62770732018-12-20 Cereal aphids differently affect benzoxazinoid levels in durum wheat Shavit, Reut Batyrshina, Zhaniya S. Dotan, Nitsan Tzin, Vered PLoS One Research Article Aphids are major pests in cereal crops that cause direct and indirect damage leading to yield reduction. Despite the fact that wheat provides 20% of the world’s caloric and protein diet, its metabolic responses to aphid attack, in general, and specifically its production of benzoxazinoid defense compounds are poorly understood. The objective of this study was to compare the metabolic diversity of durum wheat seedlings (Triticum turgidum ssp. durum) under attack by three different cereal aphids: i) the English grain aphid (Sitobion avenae Fabricius), ii) the bird cherry-oat aphid (Rhopalosiphum padi L.), and iii) the greenbug aphid (Schizaphis graminum Rondani), which are some of the most destructive aphid species to wheat. Insect progeny bioassays and metabolic analyses using chromatography/Q-Exactive/mass spectrometry non-targeted metabolomics and a targeted benzoxazinoid profile were performed on infested leaves. The insect bioassays revealed that the plants were susceptible to S. graminum, resistant to S. avenae, and mildly resistant to R. padi. The metabolic analyses of benzoxazinoids suggested that the predominant metabolites DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin- 3-one) and its glycosylated form DIMBOA-glucoside (Glc) were significantly induced upon both S. avenae, and R. padi aphid feeding. However, the levels of the benzoxazinoid metabolite HDMBOA-Glc (2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside) were enhanced due to the feeding of S. avenae and S. graminum aphids, to which Svevo was the most resistant and the most susceptible, respectively. The results showed a partial correlation between the induction of benzoxazinoids and aphid reproduction. Overall, our observations revealed diverse metabolic responses of wheat seedlings to cereal aphid feeding. Public Library of Science 2018-12-03 /pmc/articles/PMC6277073/ /pubmed/30507950 http://dx.doi.org/10.1371/journal.pone.0208103 Text en © 2018 Shavit et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shavit, Reut
Batyrshina, Zhaniya S.
Dotan, Nitsan
Tzin, Vered
Cereal aphids differently affect benzoxazinoid levels in durum wheat
title Cereal aphids differently affect benzoxazinoid levels in durum wheat
title_full Cereal aphids differently affect benzoxazinoid levels in durum wheat
title_fullStr Cereal aphids differently affect benzoxazinoid levels in durum wheat
title_full_unstemmed Cereal aphids differently affect benzoxazinoid levels in durum wheat
title_short Cereal aphids differently affect benzoxazinoid levels in durum wheat
title_sort cereal aphids differently affect benzoxazinoid levels in durum wheat
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277073/
https://www.ncbi.nlm.nih.gov/pubmed/30507950
http://dx.doi.org/10.1371/journal.pone.0208103
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