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Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss
Organ renewal is governed by the dynamics of cell division, differentiation and loss. To study these dynamics in real time, we present a platform for extended live imaging of the adult Drosophila midgut, a premier genetic model for stem-cell-based organs. A window cut into a living animal allows the...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277200/ https://www.ncbi.nlm.nih.gov/pubmed/30427308 http://dx.doi.org/10.7554/eLife.36248 |
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author | Martin, Judy Lisette Sanders, Erin Nicole Moreno-Roman, Paola Jaramillo Koyama, Leslie Ann Balachandra, Shruthi Du, XinXin O'Brien, Lucy Erin |
author_facet | Martin, Judy Lisette Sanders, Erin Nicole Moreno-Roman, Paola Jaramillo Koyama, Leslie Ann Balachandra, Shruthi Du, XinXin O'Brien, Lucy Erin |
author_sort | Martin, Judy Lisette |
collection | PubMed |
description | Organ renewal is governed by the dynamics of cell division, differentiation and loss. To study these dynamics in real time, we present a platform for extended live imaging of the adult Drosophila midgut, a premier genetic model for stem-cell-based organs. A window cut into a living animal allows the midgut to be imaged while intact and physiologically functioning. This approach prolongs imaging sessions to 12–16 hr and yields movies that document cell and tissue dynamics at vivid spatiotemporal resolution. By applying a pipeline for movie processing and analysis, we uncover new and intriguing cell behaviors: that mitotic stem cells dynamically re-orient, that daughter cells use slow kinetics of Notch activation to reach a fate-specifying threshold, and that enterocytes extrude via ratcheted constriction of a junctional ring. By enabling real-time study of midgut phenomena that were previously inaccessible, our platform opens a new realm for dynamic understanding of adult organ renewal. |
format | Online Article Text |
id | pubmed-6277200 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-62772002018-12-05 Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss Martin, Judy Lisette Sanders, Erin Nicole Moreno-Roman, Paola Jaramillo Koyama, Leslie Ann Balachandra, Shruthi Du, XinXin O'Brien, Lucy Erin eLife Stem Cells and Regenerative Medicine Organ renewal is governed by the dynamics of cell division, differentiation and loss. To study these dynamics in real time, we present a platform for extended live imaging of the adult Drosophila midgut, a premier genetic model for stem-cell-based organs. A window cut into a living animal allows the midgut to be imaged while intact and physiologically functioning. This approach prolongs imaging sessions to 12–16 hr and yields movies that document cell and tissue dynamics at vivid spatiotemporal resolution. By applying a pipeline for movie processing and analysis, we uncover new and intriguing cell behaviors: that mitotic stem cells dynamically re-orient, that daughter cells use slow kinetics of Notch activation to reach a fate-specifying threshold, and that enterocytes extrude via ratcheted constriction of a junctional ring. By enabling real-time study of midgut phenomena that were previously inaccessible, our platform opens a new realm for dynamic understanding of adult organ renewal. eLife Sciences Publications, Ltd 2018-11-14 /pmc/articles/PMC6277200/ /pubmed/30427308 http://dx.doi.org/10.7554/eLife.36248 Text en © 2018, Martin et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Stem Cells and Regenerative Medicine Martin, Judy Lisette Sanders, Erin Nicole Moreno-Roman, Paola Jaramillo Koyama, Leslie Ann Balachandra, Shruthi Du, XinXin O'Brien, Lucy Erin Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss |
title | Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss |
title_full | Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss |
title_fullStr | Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss |
title_full_unstemmed | Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss |
title_short | Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss |
title_sort | long-term live imaging of the drosophila adult midgut reveals real-time dynamics of division, differentiation and loss |
topic | Stem Cells and Regenerative Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277200/ https://www.ncbi.nlm.nih.gov/pubmed/30427308 http://dx.doi.org/10.7554/eLife.36248 |
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