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Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss

Organ renewal is governed by the dynamics of cell division, differentiation and loss. To study these dynamics in real time, we present a platform for extended live imaging of the adult Drosophila midgut, a premier genetic model for stem-cell-based organs. A window cut into a living animal allows the...

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Autores principales: Martin, Judy Lisette, Sanders, Erin Nicole, Moreno-Roman, Paola, Jaramillo Koyama, Leslie Ann, Balachandra, Shruthi, Du, XinXin, O'Brien, Lucy Erin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277200/
https://www.ncbi.nlm.nih.gov/pubmed/30427308
http://dx.doi.org/10.7554/eLife.36248
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author Martin, Judy Lisette
Sanders, Erin Nicole
Moreno-Roman, Paola
Jaramillo Koyama, Leslie Ann
Balachandra, Shruthi
Du, XinXin
O'Brien, Lucy Erin
author_facet Martin, Judy Lisette
Sanders, Erin Nicole
Moreno-Roman, Paola
Jaramillo Koyama, Leslie Ann
Balachandra, Shruthi
Du, XinXin
O'Brien, Lucy Erin
author_sort Martin, Judy Lisette
collection PubMed
description Organ renewal is governed by the dynamics of cell division, differentiation and loss. To study these dynamics in real time, we present a platform for extended live imaging of the adult Drosophila midgut, a premier genetic model for stem-cell-based organs. A window cut into a living animal allows the midgut to be imaged while intact and physiologically functioning. This approach prolongs imaging sessions to 12–16 hr and yields movies that document cell and tissue dynamics at vivid spatiotemporal resolution. By applying a pipeline for movie processing and analysis, we uncover new and intriguing cell behaviors: that mitotic stem cells dynamically re-orient, that daughter cells use slow kinetics of Notch activation to reach a fate-specifying threshold, and that enterocytes extrude via ratcheted constriction of a junctional ring. By enabling real-time study of midgut phenomena that were previously inaccessible, our platform opens a new realm for dynamic understanding of adult organ renewal.
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spelling pubmed-62772002018-12-05 Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss Martin, Judy Lisette Sanders, Erin Nicole Moreno-Roman, Paola Jaramillo Koyama, Leslie Ann Balachandra, Shruthi Du, XinXin O'Brien, Lucy Erin eLife Stem Cells and Regenerative Medicine Organ renewal is governed by the dynamics of cell division, differentiation and loss. To study these dynamics in real time, we present a platform for extended live imaging of the adult Drosophila midgut, a premier genetic model for stem-cell-based organs. A window cut into a living animal allows the midgut to be imaged while intact and physiologically functioning. This approach prolongs imaging sessions to 12–16 hr and yields movies that document cell and tissue dynamics at vivid spatiotemporal resolution. By applying a pipeline for movie processing and analysis, we uncover new and intriguing cell behaviors: that mitotic stem cells dynamically re-orient, that daughter cells use slow kinetics of Notch activation to reach a fate-specifying threshold, and that enterocytes extrude via ratcheted constriction of a junctional ring. By enabling real-time study of midgut phenomena that were previously inaccessible, our platform opens a new realm for dynamic understanding of adult organ renewal. eLife Sciences Publications, Ltd 2018-11-14 /pmc/articles/PMC6277200/ /pubmed/30427308 http://dx.doi.org/10.7554/eLife.36248 Text en © 2018, Martin et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Stem Cells and Regenerative Medicine
Martin, Judy Lisette
Sanders, Erin Nicole
Moreno-Roman, Paola
Jaramillo Koyama, Leslie Ann
Balachandra, Shruthi
Du, XinXin
O'Brien, Lucy Erin
Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss
title Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss
title_full Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss
title_fullStr Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss
title_full_unstemmed Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss
title_short Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss
title_sort long-term live imaging of the drosophila adult midgut reveals real-time dynamics of division, differentiation and loss
topic Stem Cells and Regenerative Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277200/
https://www.ncbi.nlm.nih.gov/pubmed/30427308
http://dx.doi.org/10.7554/eLife.36248
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