Cargando…

Rapid characterization of secreted recombinant proteins by native mass spectrometry

Characterization of overexpressed proteins is essential for assessing their quality, and providing input for iterative redesign and optimization. This process is typically carried out following purification procedures that require pronounced cost of time and labor. Therefore, quality assessment of r...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ben-Nissan, Gili, Vimer, Shay, Warszawski, Shira, Katz, Aliza, Yona, Meital, Unger, Tamar, Peleg, Yoav, Morgenstern, David, Cohen-Dvashi, Hadas, Diskin, Ron, Fleishman, Sarel J., Sharon, Michal
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277423/ https://www.ncbi.nlm.nih.gov/pubmed/30534605 http://dx.doi.org/10.1038/s42003-018-0231-3

Ejemplares similares

Optimizing antibody affinity and stability by the automated design of the variable light-heavy chain interfaces
por: Warszawski, Shira, et al.
Publicado: (2019)

Correction: Optimizing antibody affinity and stability by the automated design of the variable light-heavy chain interfaces
por: Warszawski, Shira, et al.
Publicado: (2020)

Mass Spectrometry Analysis of Intact Proteins from Crude Samples
por: Vimer, Shay, et al.
Publicado: (2020)

Comparative Structural Analysis of 20S Proteasome Ortholog Protein Complexes by Native Mass Spectrometry
por: Vimer, Shay, et al.
Publicado: (2020)

A “Fuzzy”-Logic Language for Encoding Multiple Physical Traits in Biomolecules
por: Warszawski, Shira, et al.
Publicado: (2014)

Anti-SARS-CoV-2 immunoadhesin remains effective against Omicron and other emerging variants of concern
por: Cohen-Dvashi, Hadas, et al.
Publicado: (2022)

Mapping of the Lassa virus LAMP1 binding site reveals unique determinants not shared by other old world arenaviruses
por: Israeli, Hadar, et al.
Publicado: (2017)

Rational design of universal immunotherapy for TfR1-tropic arenaviruses
por: Cohen-Dvashi, Hadas, et al.
Publicado: (2020)

The C-terminal tail of CSNAP attenuates the CSN complex
por: Füzesi-Levi, Maria G, et al.
Publicado: (2023)

Structure of Type-I Mycobacterium tuberculosis fatty acid synthase at 3.3 Å resolution
por: Elad, Nadav, et al.
Publicado: (2018)

Designed High-Redox Potential Laccases Exhibit High Functional Diversity
por: Barber-Zucker, Shiran, et al.
Publicado: (2022)

Local Mutations Can Serve as a Game Changer for Global Protein Solvent Interaction
por: Adams, Ellen M., et al.
Publicado: (2021)

Expression of a recombinant, 4'-Phosphopantetheinylated, active M. tuberculosis fatty acid synthase I in E. coli
por: Baron, Szilvia, et al.
Publicado: (2018)

Allosteric regulation of the 20S proteasome by the Catalytic Core Regulators (CCRs) family
por: Deshmukh, Fanindra Kumar, et al.
Publicado: (2023)

What Have We Learned from Design of Function in Large Proteins?
por: Khersonsky, Olga, et al.
Publicado: (2022)

Highly active enzymes by automated combinatorial backbone assembly and sequence design
por: Lapidoth, Gideon, et al.
Publicado: (2018)

Editorial overview: Engineering and design
por: Fleishman, Sarel J., et al.
Publicado: (2022)

Structural basis for matriglycan synthesis by the LARGE1 dual glycosyltransferase
por: Katz, Michael, et al.
Publicado: (2022)

Ultrahigh specificity in a network of computationally designed protein-interaction pairs
por: Netzer, Ravit, et al.
Publicado: (2018)

A Recombinant Collagen–mRNA Platform for Controllable Protein Synthesis
por: Sun, Liping, et al.
Publicado: (2015)

Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability
por: Goldenzweig, Adi, et al.
Publicado: (2016)

Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability
por: Goldenzweig, Adi, et al.
Publicado: (2018)

A lipophilicity-based energy function for membrane-protein modelling and design
por: Weinstein, Jonathan Yaacov, et al.
Publicado: (2019)

The AbDesign computational pipeline for modular backbone assembly and design of binders and enzymes
por: Lipsh‐Sokolik, Rosalie, et al.
Publicado: (2020)

Mapping the splicing landscape of the human immune system
por: Ner-Gaon, Hadas, et al.
Publicado: (2023)

Overcoming a species-specificity barrier in development of an inhibitory antibody targeting a modulator of tumor stroma
por: Grossman, Iris, et al.
Publicado: (2016)

A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates
por: Vazana, Yael, et al.
Publicado: (2013)

A counter-enzyme complex regulates glutamate metabolism in Bacillus subtilis
por: Jayaraman, Vijay, et al.
Publicado: (2022)

Native Mass Spectrometry: What is in the Name?
por: Leney, Aneika C., et al.
Publicado: (2016)

High-Resolution Native Mass Spectrometry
por: Tamara, Sem, et al.
Publicado: (2021)

Immune-Related Thyroiditis as a Predictor for Survival in Metastatic Renal Cell Carcinoma
por: Sagie, Shira, et al.
Publicado: (2022)

RCC Real-World Data: Prognostic Factors and Risk Stratification in the Immunotherapy Era
por: Sagie, Shira, et al.
Publicado: (2022)

Parsimonious Charge Deconvolution for Native Mass Spectrometry
por: Bern, Marshall, et al.
Publicado: (2018)

OTHR-03. Brain Metastasis Among Ovarian Cancer Patients
por: Limon, Dror, et al.
Publicado: (2021)

Computational Enzyme Engineering Pipelines for Optimized Production of Renewable Chemicals
por: Scherer, Marc, et al.
Publicado: (2021)

Did the U.S. Response to the Marathon Bombings Help or Harm Security?
por: Peleg, Kobi, et al.
Publicado: (2014)

Directed Evolution of Therapeutic Antibodies Targeting Glycosylation in Cancer
por: Amon, Ron, et al.
Publicado: (2020)

Low-back pain, lassitude and loss of appetite
por: Schattner, Ami, et al.
Publicado: (2014)

Mutational scanning reveals the determinants of protein insertion and association energetics in the plasma membrane
por: Elazar, Assaf, et al.
Publicado: (2016)

Regulating the 20S Proteasome Ubiquitin-Independent Degradation Pathway
por: Ben-Nissan, Gili, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_qcsk0r9g7hrbb8csqp238vu370