Cargando…

Biofilm Producing Clinical Staphylococcus aureus Isolates Augmented Prevalence of Antibiotic Resistant Cases in Tertiary Care Hospitals of Nepal

Staphylococcus aureus, a notorious human pathogen, is a major cause of the community as well as healthcare associated infections. It can cause a diversity of recalcitrant infections mainly due to the acquisition of resistance to multiple drugs, its diverse range of virulence factors, and the ability...

Descripción completa

Detalles Bibliográficos
Autores principales: Manandhar, Sarita, Singh, Anjana, Varma, Ajit, Pandey, Shanti, Shrivastava, Neeraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277500/
https://www.ncbi.nlm.nih.gov/pubmed/30538678
http://dx.doi.org/10.3389/fmicb.2018.02749
_version_ 1783378165238857728
author Manandhar, Sarita
Singh, Anjana
Varma, Ajit
Pandey, Shanti
Shrivastava, Neeraj
author_facet Manandhar, Sarita
Singh, Anjana
Varma, Ajit
Pandey, Shanti
Shrivastava, Neeraj
author_sort Manandhar, Sarita
collection PubMed
description Staphylococcus aureus, a notorious human pathogen, is a major cause of the community as well as healthcare associated infections. It can cause a diversity of recalcitrant infections mainly due to the acquisition of resistance to multiple drugs, its diverse range of virulence factors, and the ability to produce biofilm in indwelling medical devices. Such biofilm associated chronic infections often lead to increase in morbidity and mortality posing a high socio-economic burden, especially in developing countries. Since biofilm formation and antibiotic resistance function dependent on each other, detection of biofilm expression in clinical isolates would be advantageous in treatment decision. In this premise, we attempt to investigate the biofilm formation and its association with antibiotic resistance in clinical isolates from the patients visiting tertiary health care hospitals in Nepal. Bacterial cells isolated from clinical samples identified as S. aureus were examined for in-vitro biofilm production using both phenotypic and genotypic assays. The S. aureus isolates were also examined for susceptibility patterns of clinically relevant antibiotics as well as inducible clindamycin resistance using standard microbiological techniques and D-test, respectively. Among 161 S. aureus isolates, 131 (81.4%) were methicillin resistant S. aureus (MRSA) and 30 (18.6%) were methicillin sensitive S. aureus (MSSA) strains. Although a majority of MRSA strains (69.6%) showed inducible clindamycin resistance, almost all isolates (97% and 94%) were sensitive toward chloramphenicol and tetracycline, respectively. Detection of in vitro production of biofilm revealed the association of biofilm with methicillin as well as inducible clindamycin resistance among the clinical S. aureus isolates.
format Online
Article
Text
id pubmed-6277500
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-62775002018-12-11 Biofilm Producing Clinical Staphylococcus aureus Isolates Augmented Prevalence of Antibiotic Resistant Cases in Tertiary Care Hospitals of Nepal Manandhar, Sarita Singh, Anjana Varma, Ajit Pandey, Shanti Shrivastava, Neeraj Front Microbiol Microbiology Staphylococcus aureus, a notorious human pathogen, is a major cause of the community as well as healthcare associated infections. It can cause a diversity of recalcitrant infections mainly due to the acquisition of resistance to multiple drugs, its diverse range of virulence factors, and the ability to produce biofilm in indwelling medical devices. Such biofilm associated chronic infections often lead to increase in morbidity and mortality posing a high socio-economic burden, especially in developing countries. Since biofilm formation and antibiotic resistance function dependent on each other, detection of biofilm expression in clinical isolates would be advantageous in treatment decision. In this premise, we attempt to investigate the biofilm formation and its association with antibiotic resistance in clinical isolates from the patients visiting tertiary health care hospitals in Nepal. Bacterial cells isolated from clinical samples identified as S. aureus were examined for in-vitro biofilm production using both phenotypic and genotypic assays. The S. aureus isolates were also examined for susceptibility patterns of clinically relevant antibiotics as well as inducible clindamycin resistance using standard microbiological techniques and D-test, respectively. Among 161 S. aureus isolates, 131 (81.4%) were methicillin resistant S. aureus (MRSA) and 30 (18.6%) were methicillin sensitive S. aureus (MSSA) strains. Although a majority of MRSA strains (69.6%) showed inducible clindamycin resistance, almost all isolates (97% and 94%) were sensitive toward chloramphenicol and tetracycline, respectively. Detection of in vitro production of biofilm revealed the association of biofilm with methicillin as well as inducible clindamycin resistance among the clinical S. aureus isolates. Frontiers Media S.A. 2018-11-27 /pmc/articles/PMC6277500/ /pubmed/30538678 http://dx.doi.org/10.3389/fmicb.2018.02749 Text en Copyright © 2018 Manandhar, Singh, Varma, Pandey and Shrivastava. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Manandhar, Sarita
Singh, Anjana
Varma, Ajit
Pandey, Shanti
Shrivastava, Neeraj
Biofilm Producing Clinical Staphylococcus aureus Isolates Augmented Prevalence of Antibiotic Resistant Cases in Tertiary Care Hospitals of Nepal
title Biofilm Producing Clinical Staphylococcus aureus Isolates Augmented Prevalence of Antibiotic Resistant Cases in Tertiary Care Hospitals of Nepal
title_full Biofilm Producing Clinical Staphylococcus aureus Isolates Augmented Prevalence of Antibiotic Resistant Cases in Tertiary Care Hospitals of Nepal
title_fullStr Biofilm Producing Clinical Staphylococcus aureus Isolates Augmented Prevalence of Antibiotic Resistant Cases in Tertiary Care Hospitals of Nepal
title_full_unstemmed Biofilm Producing Clinical Staphylococcus aureus Isolates Augmented Prevalence of Antibiotic Resistant Cases in Tertiary Care Hospitals of Nepal
title_short Biofilm Producing Clinical Staphylococcus aureus Isolates Augmented Prevalence of Antibiotic Resistant Cases in Tertiary Care Hospitals of Nepal
title_sort biofilm producing clinical staphylococcus aureus isolates augmented prevalence of antibiotic resistant cases in tertiary care hospitals of nepal
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277500/
https://www.ncbi.nlm.nih.gov/pubmed/30538678
http://dx.doi.org/10.3389/fmicb.2018.02749
work_keys_str_mv AT manandharsarita biofilmproducingclinicalstaphylococcusaureusisolatesaugmentedprevalenceofantibioticresistantcasesintertiarycarehospitalsofnepal
AT singhanjana biofilmproducingclinicalstaphylococcusaureusisolatesaugmentedprevalenceofantibioticresistantcasesintertiarycarehospitalsofnepal
AT varmaajit biofilmproducingclinicalstaphylococcusaureusisolatesaugmentedprevalenceofantibioticresistantcasesintertiarycarehospitalsofnepal
AT pandeyshanti biofilmproducingclinicalstaphylococcusaureusisolatesaugmentedprevalenceofantibioticresistantcasesintertiarycarehospitalsofnepal
AT shrivastavaneeraj biofilmproducingclinicalstaphylococcusaureusisolatesaugmentedprevalenceofantibioticresistantcasesintertiarycarehospitalsofnepal