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Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)

In Portugal, the epidemiological stage for the spread of carbapenemase-producing Enterobacteriaceae (CPE) increased from sporadic isolates or single hospital clones (2010–2013), to hospital outbreaks, later. Here we report data from a 6-month study performed under the European Survey on Carbapenemas...

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Autores principales: Manageiro, Vera, Romão, Raquel, Moura, Inês Barata, Sampaio, Daniel A., Vieira, Luís, Ferreira, Eugénia, Caniça, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277554/
https://www.ncbi.nlm.nih.gov/pubmed/30538682
http://dx.doi.org/10.3389/fmicb.2018.02834
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author Manageiro, Vera
Romão, Raquel
Moura, Inês Barata
Sampaio, Daniel A.
Vieira, Luís
Ferreira, Eugénia
Caniça, Manuela
author_facet Manageiro, Vera
Romão, Raquel
Moura, Inês Barata
Sampaio, Daniel A.
Vieira, Luís
Ferreira, Eugénia
Caniça, Manuela
author_sort Manageiro, Vera
collection PubMed
description In Portugal, the epidemiological stage for the spread of carbapenemase-producing Enterobacteriaceae (CPE) increased from sporadic isolates or single hospital clones (2010–2013), to hospital outbreaks, later. Here we report data from a 6-month study performed under the European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE). During the study period, 67 isolates (61 Klebsiella pneumoniae and 6 Escherichia coli) non-susceptible to carbapenems were identified in participant hospital laboratories. We detected 37 bla(KPC–type) (including one new variant: bla(KPC–21)), 1 bla(GES–5), and 1 bla(GES–6) plus bla(KPC–3), alone or in combination with other bla genes. Bioinformatics analysis of the KPC-21-producing E. coli identified the new variant bla(KPC–21) in a 12,748 bp length plasmid. The bla(KPC–21) gene was harbored on a non-Tn4401 element, presenting upstream a partial ISKpn6 (ΔISKpn6/ΔtraN) with the related left IR (IR(L)) and downstream a truncated Tn3 transposon. PFGE and MLST analysis showed an important diversity, as isolates belonged to distinct PFGE and STs profiles. In this study, we highlighted the presence of the high-risk clone E. coli sequence-type (ST) 131 clade C/H30. This worldwide disseminated E. coli lineage was already detected in Portugal among other antibiotic resistance reservoirs. This study highlights the intra- and inter-hospital spread and possible intercontinental circulation of CPE isolates.
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spelling pubmed-62775542018-12-11 Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) Manageiro, Vera Romão, Raquel Moura, Inês Barata Sampaio, Daniel A. Vieira, Luís Ferreira, Eugénia Caniça, Manuela Front Microbiol Microbiology In Portugal, the epidemiological stage for the spread of carbapenemase-producing Enterobacteriaceae (CPE) increased from sporadic isolates or single hospital clones (2010–2013), to hospital outbreaks, later. Here we report data from a 6-month study performed under the European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE). During the study period, 67 isolates (61 Klebsiella pneumoniae and 6 Escherichia coli) non-susceptible to carbapenems were identified in participant hospital laboratories. We detected 37 bla(KPC–type) (including one new variant: bla(KPC–21)), 1 bla(GES–5), and 1 bla(GES–6) plus bla(KPC–3), alone or in combination with other bla genes. Bioinformatics analysis of the KPC-21-producing E. coli identified the new variant bla(KPC–21) in a 12,748 bp length plasmid. The bla(KPC–21) gene was harbored on a non-Tn4401 element, presenting upstream a partial ISKpn6 (ΔISKpn6/ΔtraN) with the related left IR (IR(L)) and downstream a truncated Tn3 transposon. PFGE and MLST analysis showed an important diversity, as isolates belonged to distinct PFGE and STs profiles. In this study, we highlighted the presence of the high-risk clone E. coli sequence-type (ST) 131 clade C/H30. This worldwide disseminated E. coli lineage was already detected in Portugal among other antibiotic resistance reservoirs. This study highlights the intra- and inter-hospital spread and possible intercontinental circulation of CPE isolates. Frontiers Media S.A. 2018-11-27 /pmc/articles/PMC6277554/ /pubmed/30538682 http://dx.doi.org/10.3389/fmicb.2018.02834 Text en Copyright © 2018 Manageiro, Romão, Moura, Sampaio, Vieira, Ferreira, the Network EuSCAPE-Portugal and Caniça. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Manageiro, Vera
Romão, Raquel
Moura, Inês Barata
Sampaio, Daniel A.
Vieira, Luís
Ferreira, Eugénia
Caniça, Manuela
Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
title Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
title_full Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
title_fullStr Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
title_full_unstemmed Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
title_short Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
title_sort molecular epidemiology and risk factors of carbapenemase-producing enterobacteriaceae isolates in portuguese hospitals: results from european survey on carbapenemase-producing enterobacteriaceae (euscape)
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277554/
https://www.ncbi.nlm.nih.gov/pubmed/30538682
http://dx.doi.org/10.3389/fmicb.2018.02834
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