Cargando…

CRISPR and Target-Specific DNA Endonucleases for Efficient DNA Knock-in in Eukaryotic Genomes

The discovery and mechanistic understanding of target-specific genome engineering technologies has led to extremely effective and specific genome editing in higher organisms. Target-specific genetic modification technology is expected to have a leading position in future gene therapy development, an...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Seung Hwan, Kim, Sunghyun, Hur, Junho K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277560/
https://www.ncbi.nlm.nih.gov/pubmed/30486613
http://dx.doi.org/10.14348/molcells.2018.0408
_version_ 1783378177519779840
author Lee, Seung Hwan
Kim, Sunghyun
Hur, Junho K
author_facet Lee, Seung Hwan
Kim, Sunghyun
Hur, Junho K
author_sort Lee, Seung Hwan
collection PubMed
description The discovery and mechanistic understanding of target-specific genome engineering technologies has led to extremely effective and specific genome editing in higher organisms. Target-specific genetic modification technology is expected to have a leading position in future gene therapy development, and has a ripple effect on various basic and applied studies. However, several problems remain and hinder efficient and specific editing of target genomic loci. The issues are particularly critical in precise targeted insertion of external DNA sequences into genomes. Here, we discuss some recent efforts to overcome such problems and present a perspective of future genome editing technologies.
format Online
Article
Text
id pubmed-6277560
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Korean Society for Molecular and Cellular Biology
record_format MEDLINE/PubMed
spelling pubmed-62775602018-12-13 CRISPR and Target-Specific DNA Endonucleases for Efficient DNA Knock-in in Eukaryotic Genomes Lee, Seung Hwan Kim, Sunghyun Hur, Junho K Mol Cells Minireview The discovery and mechanistic understanding of target-specific genome engineering technologies has led to extremely effective and specific genome editing in higher organisms. Target-specific genetic modification technology is expected to have a leading position in future gene therapy development, and has a ripple effect on various basic and applied studies. However, several problems remain and hinder efficient and specific editing of target genomic loci. The issues are particularly critical in precise targeted insertion of external DNA sequences into genomes. Here, we discuss some recent efforts to overcome such problems and present a perspective of future genome editing technologies. Korean Society for Molecular and Cellular Biology 2018-11-30 2018-11-14 /pmc/articles/PMC6277560/ /pubmed/30486613 http://dx.doi.org/10.14348/molcells.2018.0408 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Minireview
Lee, Seung Hwan
Kim, Sunghyun
Hur, Junho K
CRISPR and Target-Specific DNA Endonucleases for Efficient DNA Knock-in in Eukaryotic Genomes
title CRISPR and Target-Specific DNA Endonucleases for Efficient DNA Knock-in in Eukaryotic Genomes
title_full CRISPR and Target-Specific DNA Endonucleases for Efficient DNA Knock-in in Eukaryotic Genomes
title_fullStr CRISPR and Target-Specific DNA Endonucleases for Efficient DNA Knock-in in Eukaryotic Genomes
title_full_unstemmed CRISPR and Target-Specific DNA Endonucleases for Efficient DNA Knock-in in Eukaryotic Genomes
title_short CRISPR and Target-Specific DNA Endonucleases for Efficient DNA Knock-in in Eukaryotic Genomes
title_sort crispr and target-specific dna endonucleases for efficient dna knock-in in eukaryotic genomes
topic Minireview
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277560/
https://www.ncbi.nlm.nih.gov/pubmed/30486613
http://dx.doi.org/10.14348/molcells.2018.0408
work_keys_str_mv AT leeseunghwan crisprandtargetspecificdnaendonucleasesforefficientdnaknockinineukaryoticgenomes
AT kimsunghyun crisprandtargetspecificdnaendonucleasesforefficientdnaknockinineukaryoticgenomes
AT hurjunhok crisprandtargetspecificdnaendonucleasesforefficientdnaknockinineukaryoticgenomes