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Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women
OBJECTIVE: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women. METHODS: Immature oocytes were obtained by needle aspiration from 49 pregnant women (group 1) who underwent a...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Reproductive Medicine
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277669/ https://www.ncbi.nlm.nih.gov/pubmed/30538950 http://dx.doi.org/10.5653/cerm.2018.45.4.189 |
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author | Pongsuthirak, Pallop Vutyavanich, Teraporn |
author_facet | Pongsuthirak, Pallop Vutyavanich, Teraporn |
author_sort | Pongsuthirak, Pallop |
collection | PubMed |
description | OBJECTIVE: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women. METHODS: Immature oocytes were obtained by needle aspiration from 49 pregnant women (group 1) who underwent a cesarean section at term and 77 non-pregnant women (group 2) who underwent a gynecological operation during the same period (8 months). Healthy immature oocytes (530 in group 1 and 539 in group 2) were cultured and assessed for maturation 36 hours later. Mature oocytes were inseminated by intracytoplasmic sperm injection and cultured up to 144 hours. RESULTS: The percentage of degenerated oocytes was significantly higher (12.1% vs. 6.3%; p<0.001) in group 1 than in group 2. There was no significant difference in the maturation rate (66.8% vs. 68.1%; p=0.698), fertilization rate (66.7% vs. 67.6%; p=0.857), or the rate of formation of good-quality blastocysts (46.2% vs. 47.2%; p=0.898) in oocytes obtained from pregnant and non-pregnant women. CONCLUSION: The developmental competence of immature oocytes did not differ between pregnant and non-pregnant women. |
format | Online Article Text |
id | pubmed-6277669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Korean Society for Reproductive Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-62776692018-12-11 Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women Pongsuthirak, Pallop Vutyavanich, Teraporn Clin Exp Reprod Med Original Article OBJECTIVE: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women. METHODS: Immature oocytes were obtained by needle aspiration from 49 pregnant women (group 1) who underwent a cesarean section at term and 77 non-pregnant women (group 2) who underwent a gynecological operation during the same period (8 months). Healthy immature oocytes (530 in group 1 and 539 in group 2) were cultured and assessed for maturation 36 hours later. Mature oocytes were inseminated by intracytoplasmic sperm injection and cultured up to 144 hours. RESULTS: The percentage of degenerated oocytes was significantly higher (12.1% vs. 6.3%; p<0.001) in group 1 than in group 2. There was no significant difference in the maturation rate (66.8% vs. 68.1%; p=0.698), fertilization rate (66.7% vs. 67.6%; p=0.857), or the rate of formation of good-quality blastocysts (46.2% vs. 47.2%; p=0.898) in oocytes obtained from pregnant and non-pregnant women. CONCLUSION: The developmental competence of immature oocytes did not differ between pregnant and non-pregnant women. The Korean Society for Reproductive Medicine 2018-12 2018-11-30 /pmc/articles/PMC6277669/ /pubmed/30538950 http://dx.doi.org/10.5653/cerm.2018.45.4.189 Text en Copyright © 2018. The Korean Society for Reproductive Medicine http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Pongsuthirak, Pallop Vutyavanich, Teraporn Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women |
title | Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women |
title_full | Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women |
title_fullStr | Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women |
title_full_unstemmed | Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women |
title_short | Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women |
title_sort | developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277669/ https://www.ncbi.nlm.nih.gov/pubmed/30538950 http://dx.doi.org/10.5653/cerm.2018.45.4.189 |
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