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Novel method of histopathological analysis after testicular sperm extraction in patients with nonobstructive and obstructive azoospermia
OBJECTIVE: To assess whether the “testicular pool” could be used for histological analysis and whether it gave more accurate information than the standard testicular biopsy. METHODS: Between January 2017 and March 2018, this single-center prospective study included 60 azoospermic men undergoing conv...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Reproductive Medicine
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277673/ https://www.ncbi.nlm.nih.gov/pubmed/30538947 http://dx.doi.org/10.5653/cerm.2018.45.4.170 |
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author | Cito, Gianmartin Coccia, Maria Elisabetta Picone, Rita Nesi, Gabriella Cocci, Andrea Dabizzi, Sara Garaffa, Giulio Fucci, Rossella Falcone, Patrizia Bertocci, Francesco Santi, Raffaella Criscuoli, Luciana Serni, Sergio Carini, Marco Natali, Alessandro |
author_facet | Cito, Gianmartin Coccia, Maria Elisabetta Picone, Rita Nesi, Gabriella Cocci, Andrea Dabizzi, Sara Garaffa, Giulio Fucci, Rossella Falcone, Patrizia Bertocci, Francesco Santi, Raffaella Criscuoli, Luciana Serni, Sergio Carini, Marco Natali, Alessandro |
author_sort | Cito, Gianmartin |
collection | PubMed |
description | OBJECTIVE: To assess whether the “testicular pool” could be used for histological analysis and whether it gave more accurate information than the standard testicular biopsy. METHODS: Between January 2017 and March 2018, this single-center prospective study included 60 azoospermic men undergoing conventional bilateral testicular sperm extraction. Six samples were excised from each testicle and transferred to an embryologist. One additional biopsy was randomly taken from each testis for a histological analysis. After processing, the testicular pool was also sent for a histological analysis, which showed normal spermatogenesis (NS), hypospermatogenesis (HYPO), maturation arrest (MA), Sertoli cell-only syndrome (SCOS), and tubular atrophy (TA). RESULTS: Twenty of the 60 patients (33.3%) had obstructive azoospermia (OA), while the remaining 40 (66.6%) had nonobstructive azoospermia. Their mean age was 40.5 years. All patients with OA had previously undergone unsuccessful testicular fine-needle aspiration. Successful sperm retrieval (SSR) occurred in 93.3% of patients. Histological analysis of the testicular biopsy revealed NS in 12 patients (20%), HYPO and TA in 28 patients (46.6%), MA in eight patients (13.3%), and SCOS in 12 patients (20%). The testicular pool analysis showed NS in 12 patients (20%), HYPO and TA in 44 patients (73.3%), MA in four patients (6.6%), and SCOS in no patients. In four patients with MA (6.6% of the total sample) and 12 patients with SCOS (20% of the total sample) according to the standard testicular biopsy, the embryologist found SSR with cryopreservation. Overall, in 44 patients (73.3%), the testicular pool analysis confirmed the histological findings of the standard testicular biopsy. In the 16 cases (26.6%) with a discrepancy between the single-biopsy histological findings and SSR, the testicular pool analysis confirmed the embryological data on SSR. CONCLUSION: The testicular pool proved to be easily analyzable, practical, manageable, and more accurate for predicting sperm retrieval than standard testicular biopsy. |
format | Online Article Text |
id | pubmed-6277673 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Korean Society for Reproductive Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-62776732018-12-11 Novel method of histopathological analysis after testicular sperm extraction in patients with nonobstructive and obstructive azoospermia Cito, Gianmartin Coccia, Maria Elisabetta Picone, Rita Nesi, Gabriella Cocci, Andrea Dabizzi, Sara Garaffa, Giulio Fucci, Rossella Falcone, Patrizia Bertocci, Francesco Santi, Raffaella Criscuoli, Luciana Serni, Sergio Carini, Marco Natali, Alessandro Clin Exp Reprod Med Original Article OBJECTIVE: To assess whether the “testicular pool” could be used for histological analysis and whether it gave more accurate information than the standard testicular biopsy. METHODS: Between January 2017 and March 2018, this single-center prospective study included 60 azoospermic men undergoing conventional bilateral testicular sperm extraction. Six samples were excised from each testicle and transferred to an embryologist. One additional biopsy was randomly taken from each testis for a histological analysis. After processing, the testicular pool was also sent for a histological analysis, which showed normal spermatogenesis (NS), hypospermatogenesis (HYPO), maturation arrest (MA), Sertoli cell-only syndrome (SCOS), and tubular atrophy (TA). RESULTS: Twenty of the 60 patients (33.3%) had obstructive azoospermia (OA), while the remaining 40 (66.6%) had nonobstructive azoospermia. Their mean age was 40.5 years. All patients with OA had previously undergone unsuccessful testicular fine-needle aspiration. Successful sperm retrieval (SSR) occurred in 93.3% of patients. Histological analysis of the testicular biopsy revealed NS in 12 patients (20%), HYPO and TA in 28 patients (46.6%), MA in eight patients (13.3%), and SCOS in 12 patients (20%). The testicular pool analysis showed NS in 12 patients (20%), HYPO and TA in 44 patients (73.3%), MA in four patients (6.6%), and SCOS in no patients. In four patients with MA (6.6% of the total sample) and 12 patients with SCOS (20% of the total sample) according to the standard testicular biopsy, the embryologist found SSR with cryopreservation. Overall, in 44 patients (73.3%), the testicular pool analysis confirmed the histological findings of the standard testicular biopsy. In the 16 cases (26.6%) with a discrepancy between the single-biopsy histological findings and SSR, the testicular pool analysis confirmed the embryological data on SSR. CONCLUSION: The testicular pool proved to be easily analyzable, practical, manageable, and more accurate for predicting sperm retrieval than standard testicular biopsy. The Korean Society for Reproductive Medicine 2018-12 2018-11-30 /pmc/articles/PMC6277673/ /pubmed/30538947 http://dx.doi.org/10.5653/cerm.2018.45.4.170 Text en Copyright © 2018. The Korean Society for Reproductive Medicine http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Cito, Gianmartin Coccia, Maria Elisabetta Picone, Rita Nesi, Gabriella Cocci, Andrea Dabizzi, Sara Garaffa, Giulio Fucci, Rossella Falcone, Patrizia Bertocci, Francesco Santi, Raffaella Criscuoli, Luciana Serni, Sergio Carini, Marco Natali, Alessandro Novel method of histopathological analysis after testicular sperm extraction in patients with nonobstructive and obstructive azoospermia |
title | Novel method of histopathological analysis after testicular sperm extraction in patients with nonobstructive and obstructive azoospermia |
title_full | Novel method of histopathological analysis after testicular sperm extraction in patients with nonobstructive and obstructive azoospermia |
title_fullStr | Novel method of histopathological analysis after testicular sperm extraction in patients with nonobstructive and obstructive azoospermia |
title_full_unstemmed | Novel method of histopathological analysis after testicular sperm extraction in patients with nonobstructive and obstructive azoospermia |
title_short | Novel method of histopathological analysis after testicular sperm extraction in patients with nonobstructive and obstructive azoospermia |
title_sort | novel method of histopathological analysis after testicular sperm extraction in patients with nonobstructive and obstructive azoospermia |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277673/ https://www.ncbi.nlm.nih.gov/pubmed/30538947 http://dx.doi.org/10.5653/cerm.2018.45.4.170 |
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