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Genomic Analysis of Consecutive Acinetobacter baumannii Strains From a Single Patient
Acinetobacter baumannii is one of the most important nosocomial pathogens, and thus it is required to investigate how it disseminate in hospitals and infect patients. We performed whole genome sequencing for 24 A. baumannii strains isolated successively from the blood of a single patient to evaluate...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277775/ https://www.ncbi.nlm.nih.gov/pubmed/30542330 http://dx.doi.org/10.3389/fmicb.2018.02840 |
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author | Kim, Sun Ju Kim, Yae-Jean Ko, Kwan Soo |
author_facet | Kim, Sun Ju Kim, Yae-Jean Ko, Kwan Soo |
author_sort | Kim, Sun Ju |
collection | PubMed |
description | Acinetobacter baumannii is one of the most important nosocomial pathogens, and thus it is required to investigate how it disseminate in hospitals and infect patients. We performed whole genome sequencing for 24 A. baumannii strains isolated successively from the blood of a single patient to evaluate whether repeated infections were due to re-infection or relapse infection and to investigate within-host evolution. The whole genome of the first strain, BL1, was sequenced de novo using the PacBio RSII system. BL2–BL24, were sequenced with an Illumina Hiseq4000 and mapped to the genome sequences of BL1. We identified 42 single-nucleotide variations among the strains. The SNVs differentiated the strains into three groups, BL1, BL2–BL16, and BL17–BL24, indicating that the patient suffered from re-infections or co-infections by similar, but different strains. The results also showed that A. baumannii strains in each group were rather stable at the genomic level. Our study emphasizes the importance of intensive infection control. |
format | Online Article Text |
id | pubmed-6277775 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62777752018-12-12 Genomic Analysis of Consecutive Acinetobacter baumannii Strains From a Single Patient Kim, Sun Ju Kim, Yae-Jean Ko, Kwan Soo Front Microbiol Microbiology Acinetobacter baumannii is one of the most important nosocomial pathogens, and thus it is required to investigate how it disseminate in hospitals and infect patients. We performed whole genome sequencing for 24 A. baumannii strains isolated successively from the blood of a single patient to evaluate whether repeated infections were due to re-infection or relapse infection and to investigate within-host evolution. The whole genome of the first strain, BL1, was sequenced de novo using the PacBio RSII system. BL2–BL24, were sequenced with an Illumina Hiseq4000 and mapped to the genome sequences of BL1. We identified 42 single-nucleotide variations among the strains. The SNVs differentiated the strains into three groups, BL1, BL2–BL16, and BL17–BL24, indicating that the patient suffered from re-infections or co-infections by similar, but different strains. The results also showed that A. baumannii strains in each group were rather stable at the genomic level. Our study emphasizes the importance of intensive infection control. Frontiers Media S.A. 2018-11-27 /pmc/articles/PMC6277775/ /pubmed/30542330 http://dx.doi.org/10.3389/fmicb.2018.02840 Text en Copyright © 2018 Kim, Kim and Ko. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Kim, Sun Ju Kim, Yae-Jean Ko, Kwan Soo Genomic Analysis of Consecutive Acinetobacter baumannii Strains From a Single Patient |
title | Genomic Analysis of Consecutive Acinetobacter baumannii Strains From a Single Patient |
title_full | Genomic Analysis of Consecutive Acinetobacter baumannii Strains From a Single Patient |
title_fullStr | Genomic Analysis of Consecutive Acinetobacter baumannii Strains From a Single Patient |
title_full_unstemmed | Genomic Analysis of Consecutive Acinetobacter baumannii Strains From a Single Patient |
title_short | Genomic Analysis of Consecutive Acinetobacter baumannii Strains From a Single Patient |
title_sort | genomic analysis of consecutive acinetobacter baumannii strains from a single patient |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277775/ https://www.ncbi.nlm.nih.gov/pubmed/30542330 http://dx.doi.org/10.3389/fmicb.2018.02840 |
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