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An inexpensive system for imaging the contents of multi-well plates
An inexpensive system for automated imaging of the contents of 12-, 24- and 96-well plates has been built. The xyz stage is constructed from parts from a light-duty computer numerical control wood-carving/engraving machine, and the Arduino-based board was wired so that it can trigger still images or...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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International Union of Crystallography
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277959/ https://www.ncbi.nlm.nih.gov/pubmed/30511674 http://dx.doi.org/10.1107/S2053230X18016515 |
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author | Bohm, Andrew |
author_facet | Bohm, Andrew |
author_sort | Bohm, Andrew |
collection | PubMed |
description | An inexpensive system for automated imaging of the contents of 12-, 24- and 96-well plates has been built. The xyz stage is constructed from parts from a light-duty computer numerical control wood-carving/engraving machine, and the Arduino-based board was wired so that it can trigger still images or movies though a microscope-mounted digital camera. The translation stage provides reproducible three-dimensional movement of the sample over a volume of 160 mm in x, 100 mm in y and 40 mm in z. A Python script generates the G-code command file that scans the plate and collects a series of z-stacked images of each sample. A second Python script automates the calculation of images with a digitally enhanced depth of field. The imaging system is currently being used to facilitate screening for protein crystals, but it could be used to automate the imaging of many other types of samples in multi-well plates. |
format | Online Article Text |
id | pubmed-6277959 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-62779592018-12-13 An inexpensive system for imaging the contents of multi-well plates Bohm, Andrew Acta Crystallogr F Struct Biol Commun Research Communications An inexpensive system for automated imaging of the contents of 12-, 24- and 96-well plates has been built. The xyz stage is constructed from parts from a light-duty computer numerical control wood-carving/engraving machine, and the Arduino-based board was wired so that it can trigger still images or movies though a microscope-mounted digital camera. The translation stage provides reproducible three-dimensional movement of the sample over a volume of 160 mm in x, 100 mm in y and 40 mm in z. A Python script generates the G-code command file that scans the plate and collects a series of z-stacked images of each sample. A second Python script automates the calculation of images with a digitally enhanced depth of field. The imaging system is currently being used to facilitate screening for protein crystals, but it could be used to automate the imaging of many other types of samples in multi-well plates. International Union of Crystallography 2018-11-29 /pmc/articles/PMC6277959/ /pubmed/30511674 http://dx.doi.org/10.1107/S2053230X18016515 Text en © Bohm 2018 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/ |
spellingShingle | Research Communications Bohm, Andrew An inexpensive system for imaging the contents of multi-well plates |
title | An inexpensive system for imaging the contents of multi-well plates |
title_full | An inexpensive system for imaging the contents of multi-well plates |
title_fullStr | An inexpensive system for imaging the contents of multi-well plates |
title_full_unstemmed | An inexpensive system for imaging the contents of multi-well plates |
title_short | An inexpensive system for imaging the contents of multi-well plates |
title_sort | inexpensive system for imaging the contents of multi-well plates |
topic | Research Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277959/ https://www.ncbi.nlm.nih.gov/pubmed/30511674 http://dx.doi.org/10.1107/S2053230X18016515 |
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