Cargando…

Insecticide resistance status, frequency of L1014F Kdr and G119S Ace-1 mutations, and expression of detoxification enzymes in Anopheles gambiae (s.l.) in two regions of northern Benin in preparation for indoor residual spraying

BACKGROUND: This study aims to provide baseline data on the resistance status to insecticides, the frequency of mechanisms involved and the impact of the association with the synergist piperonyl butoxide (PBO) on resistant Anopheles gambiae (s.l.) populations in two regions of northern Benin, prior...

Descripción completa

Detalles Bibliográficos
Autores principales: Salako, Albert Sourou, Ahogni, Idelphonse, Aïkpon, Rock, Sidick, Aboubakar, Dagnon, Fortune, Sovi, Arthur, Sominahouin, André Aimé, Agossa, Fiacre, Iyikirenga, Laurent, Akogbeto, Martin C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278060/
https://www.ncbi.nlm.nih.gov/pubmed/30509288
http://dx.doi.org/10.1186/s13071-018-3180-2
_version_ 1783378277212094464
author Salako, Albert Sourou
Ahogni, Idelphonse
Aïkpon, Rock
Sidick, Aboubakar
Dagnon, Fortune
Sovi, Arthur
Sominahouin, André Aimé
Agossa, Fiacre
Iyikirenga, Laurent
Akogbeto, Martin C.
author_facet Salako, Albert Sourou
Ahogni, Idelphonse
Aïkpon, Rock
Sidick, Aboubakar
Dagnon, Fortune
Sovi, Arthur
Sominahouin, André Aimé
Agossa, Fiacre
Iyikirenga, Laurent
Akogbeto, Martin C.
author_sort Salako, Albert Sourou
collection PubMed
description BACKGROUND: This study aims to provide baseline data on the resistance status to insecticides, the frequency of mechanisms involved and the impact of the association with the synergist piperonyl butoxide (PBO) on resistant Anopheles gambiae (s.l.) populations in two regions of northern Benin, prior to an indoor residual spraying campaign and introduction of next generation long-lasting insecticidal nets (LLINs) incorporating PBO. METHODS: Adult Anopheles gambiae (s.l.) originating from larvae collected in two study regions (Alibori within the Kandi-Gogounou-Segbana districts and Donga within the Djougou-Copargo-Ouake districts) were tested with impregnated papers (bendiocarb 0.1%, pirimiphos-methyl 0.25%, permethrin 0.75% and deltamethrin 0.05%). The synergist PBO was used to check for the involvement of detoxification enzymes in pyrethroid resistant populations. Molecular analyses were performed for the identification of species within the Anopheles gambiae (s.l.) complex and kdr L1014F and G119S Ace-1 mutations. Biochemical assays assessed the activity of detoxification enzymes. RESULTS: Anopheles gambiae (s.l.) was resistant to pyrethroids, with a mortality range of 25–83% with deltamethrin and 6–55% with permethrin. A significant increase in mortality was observed after pre-exposure to PBO for both deltamethrin (63–99%) and permethrin (56–99%). With bendiocarb, An. gambiae (s.l.) were susceptible in Kandi (99% mortality), with possible resistance (92–95%) recorded in Djougou, Copargo, Gogounou, Ouake and Segbana. All study populations were fully susceptible to pirimiphos-methyl. The frequencies of resistant mutations varied according to species and sites: 0.67–0.88 for L1014F kdr and 0–0.06 for G119S Ace-1. Three study locations (Djougou, Gogounou and Kandi) showed high oxidase activity and four sites (Djougou, Ouake, Copargo and Kandi) showed elevated esterase activity. CONCLUSIONS: This study confirms resistance to pyrethroids and suggests emerging bendiocarb resistance in An. gambiae (s.l.) populations in northern Benin. However, recovery of susceptibility to pyrethroids after PBO exposure, and susceptibility to organophosphates in the An. gambiae (s.l.) populations indicate that next generation LLINs incorporating PBO synergist combined with an indoor residual spraying (IRS) campaign with organophosphate insecticides may be regarded as alternative control tools.
format Online
Article
Text
id pubmed-6278060
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-62780602018-12-10 Insecticide resistance status, frequency of L1014F Kdr and G119S Ace-1 mutations, and expression of detoxification enzymes in Anopheles gambiae (s.l.) in two regions of northern Benin in preparation for indoor residual spraying Salako, Albert Sourou Ahogni, Idelphonse Aïkpon, Rock Sidick, Aboubakar Dagnon, Fortune Sovi, Arthur Sominahouin, André Aimé Agossa, Fiacre Iyikirenga, Laurent Akogbeto, Martin C. Parasit Vectors Research BACKGROUND: This study aims to provide baseline data on the resistance status to insecticides, the frequency of mechanisms involved and the impact of the association with the synergist piperonyl butoxide (PBO) on resistant Anopheles gambiae (s.l.) populations in two regions of northern Benin, prior to an indoor residual spraying campaign and introduction of next generation long-lasting insecticidal nets (LLINs) incorporating PBO. METHODS: Adult Anopheles gambiae (s.l.) originating from larvae collected in two study regions (Alibori within the Kandi-Gogounou-Segbana districts and Donga within the Djougou-Copargo-Ouake districts) were tested with impregnated papers (bendiocarb 0.1%, pirimiphos-methyl 0.25%, permethrin 0.75% and deltamethrin 0.05%). The synergist PBO was used to check for the involvement of detoxification enzymes in pyrethroid resistant populations. Molecular analyses were performed for the identification of species within the Anopheles gambiae (s.l.) complex and kdr L1014F and G119S Ace-1 mutations. Biochemical assays assessed the activity of detoxification enzymes. RESULTS: Anopheles gambiae (s.l.) was resistant to pyrethroids, with a mortality range of 25–83% with deltamethrin and 6–55% with permethrin. A significant increase in mortality was observed after pre-exposure to PBO for both deltamethrin (63–99%) and permethrin (56–99%). With bendiocarb, An. gambiae (s.l.) were susceptible in Kandi (99% mortality), with possible resistance (92–95%) recorded in Djougou, Copargo, Gogounou, Ouake and Segbana. All study populations were fully susceptible to pirimiphos-methyl. The frequencies of resistant mutations varied according to species and sites: 0.67–0.88 for L1014F kdr and 0–0.06 for G119S Ace-1. Three study locations (Djougou, Gogounou and Kandi) showed high oxidase activity and four sites (Djougou, Ouake, Copargo and Kandi) showed elevated esterase activity. CONCLUSIONS: This study confirms resistance to pyrethroids and suggests emerging bendiocarb resistance in An. gambiae (s.l.) populations in northern Benin. However, recovery of susceptibility to pyrethroids after PBO exposure, and susceptibility to organophosphates in the An. gambiae (s.l.) populations indicate that next generation LLINs incorporating PBO synergist combined with an indoor residual spraying (IRS) campaign with organophosphate insecticides may be regarded as alternative control tools. BioMed Central 2018-12-04 /pmc/articles/PMC6278060/ /pubmed/30509288 http://dx.doi.org/10.1186/s13071-018-3180-2 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Salako, Albert Sourou
Ahogni, Idelphonse
Aïkpon, Rock
Sidick, Aboubakar
Dagnon, Fortune
Sovi, Arthur
Sominahouin, André Aimé
Agossa, Fiacre
Iyikirenga, Laurent
Akogbeto, Martin C.
Insecticide resistance status, frequency of L1014F Kdr and G119S Ace-1 mutations, and expression of detoxification enzymes in Anopheles gambiae (s.l.) in two regions of northern Benin in preparation for indoor residual spraying
title Insecticide resistance status, frequency of L1014F Kdr and G119S Ace-1 mutations, and expression of detoxification enzymes in Anopheles gambiae (s.l.) in two regions of northern Benin in preparation for indoor residual spraying
title_full Insecticide resistance status, frequency of L1014F Kdr and G119S Ace-1 mutations, and expression of detoxification enzymes in Anopheles gambiae (s.l.) in two regions of northern Benin in preparation for indoor residual spraying
title_fullStr Insecticide resistance status, frequency of L1014F Kdr and G119S Ace-1 mutations, and expression of detoxification enzymes in Anopheles gambiae (s.l.) in two regions of northern Benin in preparation for indoor residual spraying
title_full_unstemmed Insecticide resistance status, frequency of L1014F Kdr and G119S Ace-1 mutations, and expression of detoxification enzymes in Anopheles gambiae (s.l.) in two regions of northern Benin in preparation for indoor residual spraying
title_short Insecticide resistance status, frequency of L1014F Kdr and G119S Ace-1 mutations, and expression of detoxification enzymes in Anopheles gambiae (s.l.) in two regions of northern Benin in preparation for indoor residual spraying
title_sort insecticide resistance status, frequency of l1014f kdr and g119s ace-1 mutations, and expression of detoxification enzymes in anopheles gambiae (s.l.) in two regions of northern benin in preparation for indoor residual spraying
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278060/
https://www.ncbi.nlm.nih.gov/pubmed/30509288
http://dx.doi.org/10.1186/s13071-018-3180-2
work_keys_str_mv AT salakoalbertsourou insecticideresistancestatusfrequencyofl1014fkdrandg119sace1mutationsandexpressionofdetoxificationenzymesinanophelesgambiaeslintworegionsofnorthernbenininpreparationforindoorresidualspraying
AT ahogniidelphonse insecticideresistancestatusfrequencyofl1014fkdrandg119sace1mutationsandexpressionofdetoxificationenzymesinanophelesgambiaeslintworegionsofnorthernbenininpreparationforindoorresidualspraying
AT aikponrock insecticideresistancestatusfrequencyofl1014fkdrandg119sace1mutationsandexpressionofdetoxificationenzymesinanophelesgambiaeslintworegionsofnorthernbenininpreparationforindoorresidualspraying
AT sidickaboubakar insecticideresistancestatusfrequencyofl1014fkdrandg119sace1mutationsandexpressionofdetoxificationenzymesinanophelesgambiaeslintworegionsofnorthernbenininpreparationforindoorresidualspraying
AT dagnonfortune insecticideresistancestatusfrequencyofl1014fkdrandg119sace1mutationsandexpressionofdetoxificationenzymesinanophelesgambiaeslintworegionsofnorthernbenininpreparationforindoorresidualspraying
AT soviarthur insecticideresistancestatusfrequencyofl1014fkdrandg119sace1mutationsandexpressionofdetoxificationenzymesinanophelesgambiaeslintworegionsofnorthernbenininpreparationforindoorresidualspraying
AT sominahouinandreaime insecticideresistancestatusfrequencyofl1014fkdrandg119sace1mutationsandexpressionofdetoxificationenzymesinanophelesgambiaeslintworegionsofnorthernbenininpreparationforindoorresidualspraying
AT agossafiacre insecticideresistancestatusfrequencyofl1014fkdrandg119sace1mutationsandexpressionofdetoxificationenzymesinanophelesgambiaeslintworegionsofnorthernbenininpreparationforindoorresidualspraying
AT iyikirengalaurent insecticideresistancestatusfrequencyofl1014fkdrandg119sace1mutationsandexpressionofdetoxificationenzymesinanophelesgambiaeslintworegionsofnorthernbenininpreparationforindoorresidualspraying
AT akogbetomartinc insecticideresistancestatusfrequencyofl1014fkdrandg119sace1mutationsandexpressionofdetoxificationenzymesinanophelesgambiaeslintworegionsofnorthernbenininpreparationforindoorresidualspraying