Cargando…

Galla Rhois water extract inhibits lung metastasis by inducing AMPK-mediated apoptosis and suppressing metastatic properties of colorectal cancer cells

Galla Rhois is a commonly used medicine in East Asia for the treatment of several diseases. However, the effects of Galla Rhois on the metastasis of colorectal cancer (CRC) and the underlying molecular mechanisms have not been studied. We investigated the anti-metastatic properties of Galla Rhois wa...

Descripción completa

Detalles Bibliográficos
Autores principales: Mun, Jeong-Geon, Kee, Ji-Ye, Han, Yo-Han, Lee, Sullim, Park, Seong-Hwan, Jeon, Hee Dong, Hong, Seung-Heon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278418/
https://www.ncbi.nlm.nih.gov/pubmed/30365120
http://dx.doi.org/10.3892/or.2018.6812
_version_ 1783378361415892992
author Mun, Jeong-Geon
Kee, Ji-Ye
Han, Yo-Han
Lee, Sullim
Park, Seong-Hwan
Jeon, Hee Dong
Hong, Seung-Heon
author_facet Mun, Jeong-Geon
Kee, Ji-Ye
Han, Yo-Han
Lee, Sullim
Park, Seong-Hwan
Jeon, Hee Dong
Hong, Seung-Heon
author_sort Mun, Jeong-Geon
collection PubMed
description Galla Rhois is a commonly used medicine in East Asia for the treatment of several diseases. However, the effects of Galla Rhois on the metastasis of colorectal cancer (CRC) and the underlying molecular mechanisms have not been studied. We investigated the anti-metastatic properties of Galla Rhois water extract (GRWE) on metastatic CRC cells. The effect of GRWE on the viability of colon 26 (CT26) cells was evaluated using WST-8 assay. Annexin V assay and western blot analysis were performed to elucidate the underlying molecular mechanisms involved in apoptosis. GRWE suppressed viability of CT26 cells by inducing apoptosis through the cleavage of caspase-3 and PARP, downregulation of caspase-8, caspase-9, Bcl-2 and Bcl-xL, and upregulation of Bax. Metastatic phenotypes such as epithelial-mesenchymal transition (EMT), migration, and invasion of CRC cells were investigated by real-time reverse transcription polymerase chain reaction, wound healing assay, and matrigel invasion assay, respectively. Non-cytotoxic concentrations of GRWE inhibited EMT in CRC cells by regulating the expression of EMT markers. GRWE attenuated cell migration and invasion through the inhibition of matrix metalloproteinase (MMP)-2 and MMP-9 activity. Moreover, GRWE suppressed colorectal lung metastasis in vivo, suggestive of its potential application for the treatment of colorectal metastasis.
format Online
Article
Text
id pubmed-6278418
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-62784182018-12-17 Galla Rhois water extract inhibits lung metastasis by inducing AMPK-mediated apoptosis and suppressing metastatic properties of colorectal cancer cells Mun, Jeong-Geon Kee, Ji-Ye Han, Yo-Han Lee, Sullim Park, Seong-Hwan Jeon, Hee Dong Hong, Seung-Heon Oncol Rep Articles Galla Rhois is a commonly used medicine in East Asia for the treatment of several diseases. However, the effects of Galla Rhois on the metastasis of colorectal cancer (CRC) and the underlying molecular mechanisms have not been studied. We investigated the anti-metastatic properties of Galla Rhois water extract (GRWE) on metastatic CRC cells. The effect of GRWE on the viability of colon 26 (CT26) cells was evaluated using WST-8 assay. Annexin V assay and western blot analysis were performed to elucidate the underlying molecular mechanisms involved in apoptosis. GRWE suppressed viability of CT26 cells by inducing apoptosis through the cleavage of caspase-3 and PARP, downregulation of caspase-8, caspase-9, Bcl-2 and Bcl-xL, and upregulation of Bax. Metastatic phenotypes such as epithelial-mesenchymal transition (EMT), migration, and invasion of CRC cells were investigated by real-time reverse transcription polymerase chain reaction, wound healing assay, and matrigel invasion assay, respectively. Non-cytotoxic concentrations of GRWE inhibited EMT in CRC cells by regulating the expression of EMT markers. GRWE attenuated cell migration and invasion through the inhibition of matrix metalloproteinase (MMP)-2 and MMP-9 activity. Moreover, GRWE suppressed colorectal lung metastasis in vivo, suggestive of its potential application for the treatment of colorectal metastasis. D.A. Spandidos 2019-01 2018-10-22 /pmc/articles/PMC6278418/ /pubmed/30365120 http://dx.doi.org/10.3892/or.2018.6812 Text en Copyright: © Mun et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Mun, Jeong-Geon
Kee, Ji-Ye
Han, Yo-Han
Lee, Sullim
Park, Seong-Hwan
Jeon, Hee Dong
Hong, Seung-Heon
Galla Rhois water extract inhibits lung metastasis by inducing AMPK-mediated apoptosis and suppressing metastatic properties of colorectal cancer cells
title Galla Rhois water extract inhibits lung metastasis by inducing AMPK-mediated apoptosis and suppressing metastatic properties of colorectal cancer cells
title_full Galla Rhois water extract inhibits lung metastasis by inducing AMPK-mediated apoptosis and suppressing metastatic properties of colorectal cancer cells
title_fullStr Galla Rhois water extract inhibits lung metastasis by inducing AMPK-mediated apoptosis and suppressing metastatic properties of colorectal cancer cells
title_full_unstemmed Galla Rhois water extract inhibits lung metastasis by inducing AMPK-mediated apoptosis and suppressing metastatic properties of colorectal cancer cells
title_short Galla Rhois water extract inhibits lung metastasis by inducing AMPK-mediated apoptosis and suppressing metastatic properties of colorectal cancer cells
title_sort galla rhois water extract inhibits lung metastasis by inducing ampk-mediated apoptosis and suppressing metastatic properties of colorectal cancer cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278418/
https://www.ncbi.nlm.nih.gov/pubmed/30365120
http://dx.doi.org/10.3892/or.2018.6812
work_keys_str_mv AT munjeonggeon gallarhoiswaterextractinhibitslungmetastasisbyinducingampkmediatedapoptosisandsuppressingmetastaticpropertiesofcolorectalcancercells
AT keejiye gallarhoiswaterextractinhibitslungmetastasisbyinducingampkmediatedapoptosisandsuppressingmetastaticpropertiesofcolorectalcancercells
AT hanyohan gallarhoiswaterextractinhibitslungmetastasisbyinducingampkmediatedapoptosisandsuppressingmetastaticpropertiesofcolorectalcancercells
AT leesullim gallarhoiswaterextractinhibitslungmetastasisbyinducingampkmediatedapoptosisandsuppressingmetastaticpropertiesofcolorectalcancercells
AT parkseonghwan gallarhoiswaterextractinhibitslungmetastasisbyinducingampkmediatedapoptosisandsuppressingmetastaticpropertiesofcolorectalcancercells
AT jeonheedong gallarhoiswaterextractinhibitslungmetastasisbyinducingampkmediatedapoptosisandsuppressingmetastaticpropertiesofcolorectalcancercells
AT hongseungheon gallarhoiswaterextractinhibitslungmetastasisbyinducingampkmediatedapoptosisandsuppressingmetastaticpropertiesofcolorectalcancercells