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Implementation of a Single Quadrupole Mass Spectrometer for Fingerprint Analysis: Venenum bufonis as a Case Study

The mass spectrometry (MS) has been widely used for profiling chemical components of traditional Chinese medicine (TCM). However, there are few studies reporting quality control of TCM based on mass spectrometry fingerprint (MSF) due to its complicated operation and high cost. The aim of this study...

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Autores principales: Wei, Wenlong, Wang, Xia, Hou, Jinjun, Yao, Changliang, Feng, Zijin, Zhang, Jianqing, Han, Sumei, Deng, Yanping, Huang, Yong, Wu, Wanying, Guo, Dean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278474/
https://www.ncbi.nlm.nih.gov/pubmed/30463184
http://dx.doi.org/10.3390/molecules23113020
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author Wei, Wenlong
Wang, Xia
Hou, Jinjun
Yao, Changliang
Feng, Zijin
Zhang, Jianqing
Han, Sumei
Deng, Yanping
Huang, Yong
Wu, Wanying
Guo, Dean
author_facet Wei, Wenlong
Wang, Xia
Hou, Jinjun
Yao, Changliang
Feng, Zijin
Zhang, Jianqing
Han, Sumei
Deng, Yanping
Huang, Yong
Wu, Wanying
Guo, Dean
author_sort Wei, Wenlong
collection PubMed
description The mass spectrometry (MS) has been widely used for profiling chemical components of traditional Chinese medicine (TCM). However, there are few studies reporting quality control of TCM based on mass spectrometry fingerprint (MSF) due to its complicated operation and high cost. The aim of this study was to extend the application of MSF for quality evaluation of TCM. In this study, an MSF based on single quadrupole mass spectrometry method was established, and was successfully used for the quality control of Venenum bufonis (VB), a famous TCM which was used clinically for cancer treatment in China. The results showed that the superiority of MSF for more chemical information exposure and the finding of more potential chemical markers (eight versus four) compared with the traditional photo-diode array (a kind of ultra violet detector, PDA). Besides, the performance of MSF was also validated by similarity and principle component analysis (PCA) of MS data acquired on two other mass spectrometry (low-resolution, triple quadrupole, QQQ, and high-resolution, quadruple time-of-flight, Q-TOF), showing high consistency with QQQ and Q-TOF, but robustness with few parameters’ settings. Based on our study, MSF could be widely applied for the quality control of TCM.
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spelling pubmed-62784742018-12-13 Implementation of a Single Quadrupole Mass Spectrometer for Fingerprint Analysis: Venenum bufonis as a Case Study Wei, Wenlong Wang, Xia Hou, Jinjun Yao, Changliang Feng, Zijin Zhang, Jianqing Han, Sumei Deng, Yanping Huang, Yong Wu, Wanying Guo, Dean Molecules Article The mass spectrometry (MS) has been widely used for profiling chemical components of traditional Chinese medicine (TCM). However, there are few studies reporting quality control of TCM based on mass spectrometry fingerprint (MSF) due to its complicated operation and high cost. The aim of this study was to extend the application of MSF for quality evaluation of TCM. In this study, an MSF based on single quadrupole mass spectrometry method was established, and was successfully used for the quality control of Venenum bufonis (VB), a famous TCM which was used clinically for cancer treatment in China. The results showed that the superiority of MSF for more chemical information exposure and the finding of more potential chemical markers (eight versus four) compared with the traditional photo-diode array (a kind of ultra violet detector, PDA). Besides, the performance of MSF was also validated by similarity and principle component analysis (PCA) of MS data acquired on two other mass spectrometry (low-resolution, triple quadrupole, QQQ, and high-resolution, quadruple time-of-flight, Q-TOF), showing high consistency with QQQ and Q-TOF, but robustness with few parameters’ settings. Based on our study, MSF could be widely applied for the quality control of TCM. MDPI 2018-11-19 /pmc/articles/PMC6278474/ /pubmed/30463184 http://dx.doi.org/10.3390/molecules23113020 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wei, Wenlong
Wang, Xia
Hou, Jinjun
Yao, Changliang
Feng, Zijin
Zhang, Jianqing
Han, Sumei
Deng, Yanping
Huang, Yong
Wu, Wanying
Guo, Dean
Implementation of a Single Quadrupole Mass Spectrometer for Fingerprint Analysis: Venenum bufonis as a Case Study
title Implementation of a Single Quadrupole Mass Spectrometer for Fingerprint Analysis: Venenum bufonis as a Case Study
title_full Implementation of a Single Quadrupole Mass Spectrometer for Fingerprint Analysis: Venenum bufonis as a Case Study
title_fullStr Implementation of a Single Quadrupole Mass Spectrometer for Fingerprint Analysis: Venenum bufonis as a Case Study
title_full_unstemmed Implementation of a Single Quadrupole Mass Spectrometer for Fingerprint Analysis: Venenum bufonis as a Case Study
title_short Implementation of a Single Quadrupole Mass Spectrometer for Fingerprint Analysis: Venenum bufonis as a Case Study
title_sort implementation of a single quadrupole mass spectrometer for fingerprint analysis: venenum bufonis as a case study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278474/
https://www.ncbi.nlm.nih.gov/pubmed/30463184
http://dx.doi.org/10.3390/molecules23113020
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