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Insight into Rapid DNA-Specific Identification of Animal Origin Based on FTIR Analysis: A Case Study
In this study, a methodology has been proposed to identify the origin of animal DNA, employing high throughput extension accessory Fourier transform infrared (HT-FTIR) spectroscopy coupled with chemometrics. Important discriminatory characteristics were identified in the FTIR spectral peaks of 51 st...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278494/ https://www.ncbi.nlm.nih.gov/pubmed/30388819 http://dx.doi.org/10.3390/molecules23112842 |
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author | Han, Yahong Jian, Lin Yao, Yumei Wang, Xinlei Han, Lujia Liu, Xian |
author_facet | Han, Yahong Jian, Lin Yao, Yumei Wang, Xinlei Han, Lujia Liu, Xian |
author_sort | Han, Yahong |
collection | PubMed |
description | In this study, a methodology has been proposed to identify the origin of animal DNA, employing high throughput extension accessory Fourier transform infrared (HT-FTIR) spectroscopy coupled with chemometrics. Important discriminatory characteristics were identified in the FTIR spectral peaks of 51 standard DNA samples (25 from bovine and 26 from fish origins), including 1710, 1659, 1608, 1531, 1404, 1375, 1248, 1091, 1060, and 966 cm(−1). In particular, the bands at 1708 and 1668 cm(−1) were higher in fish DNA than in bovine DNA, while the reverse was true for the band at 1530 cm(−1) was shown the opposite result. It was also found that the PO(2)(−) V(as)/V(s) ratio (1238/1094 cm(−1)) was significantly higher (p < 0.05) in bovine DNA than in fish DNA. These discriminatory characteristics were further revealed to be closely related to the base content and base sequences of different samples. Multivariate analyses, such as principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were conducted, and both the sensitivity and specificity values of PLS-DA model were one. This methodology has been further validated by 20 meat tissue samples (4 from bovine, 5 from ovine, 5 from porcine, and 6 from fish origins), and these were successfully differentiated. This case study demonstrated that FTIR spectroscopy coupled with PLS-DA discriminant model could provide a rapid, sensitive, and reliable approach for the identification of DNA of animal origin. This methodology could be widely applied in food, feed, forensic science, and archaeology studies. |
format | Online Article Text |
id | pubmed-6278494 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62784942018-12-13 Insight into Rapid DNA-Specific Identification of Animal Origin Based on FTIR Analysis: A Case Study Han, Yahong Jian, Lin Yao, Yumei Wang, Xinlei Han, Lujia Liu, Xian Molecules Article In this study, a methodology has been proposed to identify the origin of animal DNA, employing high throughput extension accessory Fourier transform infrared (HT-FTIR) spectroscopy coupled with chemometrics. Important discriminatory characteristics were identified in the FTIR spectral peaks of 51 standard DNA samples (25 from bovine and 26 from fish origins), including 1710, 1659, 1608, 1531, 1404, 1375, 1248, 1091, 1060, and 966 cm(−1). In particular, the bands at 1708 and 1668 cm(−1) were higher in fish DNA than in bovine DNA, while the reverse was true for the band at 1530 cm(−1) was shown the opposite result. It was also found that the PO(2)(−) V(as)/V(s) ratio (1238/1094 cm(−1)) was significantly higher (p < 0.05) in bovine DNA than in fish DNA. These discriminatory characteristics were further revealed to be closely related to the base content and base sequences of different samples. Multivariate analyses, such as principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were conducted, and both the sensitivity and specificity values of PLS-DA model were one. This methodology has been further validated by 20 meat tissue samples (4 from bovine, 5 from ovine, 5 from porcine, and 6 from fish origins), and these were successfully differentiated. This case study demonstrated that FTIR spectroscopy coupled with PLS-DA discriminant model could provide a rapid, sensitive, and reliable approach for the identification of DNA of animal origin. This methodology could be widely applied in food, feed, forensic science, and archaeology studies. MDPI 2018-11-01 /pmc/articles/PMC6278494/ /pubmed/30388819 http://dx.doi.org/10.3390/molecules23112842 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Han, Yahong Jian, Lin Yao, Yumei Wang, Xinlei Han, Lujia Liu, Xian Insight into Rapid DNA-Specific Identification of Animal Origin Based on FTIR Analysis: A Case Study |
title | Insight into Rapid DNA-Specific Identification of Animal Origin Based on FTIR Analysis: A Case Study |
title_full | Insight into Rapid DNA-Specific Identification of Animal Origin Based on FTIR Analysis: A Case Study |
title_fullStr | Insight into Rapid DNA-Specific Identification of Animal Origin Based on FTIR Analysis: A Case Study |
title_full_unstemmed | Insight into Rapid DNA-Specific Identification of Animal Origin Based on FTIR Analysis: A Case Study |
title_short | Insight into Rapid DNA-Specific Identification of Animal Origin Based on FTIR Analysis: A Case Study |
title_sort | insight into rapid dna-specific identification of animal origin based on ftir analysis: a case study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278494/ https://www.ncbi.nlm.nih.gov/pubmed/30388819 http://dx.doi.org/10.3390/molecules23112842 |
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