Cargando…

A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina

Soil fungi are known to contain a rich variety of defense metabolites that allow them to compete with other organisms (fungi, bacteria, nematodes, and insects) and help them occupy more preferential areas at the expense of effective antagonism. These compounds possess antibiotic activity towards a w...

Descripción completa

Detalles Bibliográficos
Autores principales: Rogozhin, Eugene A., Sadykova, Vera S., Baranova, Anna A., Vasilchenko, Alexey S., Lushpa, Vladislav A., Mineev, Konstantin S., Georgieva, Marina L., Kul’ko, Alexander B., Krasheninnikov, Mikhail E., Lyundup, Alexey V., Vasilchenko, Anastasia V., Andreev, Yaroslav A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278523/
https://www.ncbi.nlm.nih.gov/pubmed/30373232
http://dx.doi.org/10.3390/molecules23112785
_version_ 1783378386049040384
author Rogozhin, Eugene A.
Sadykova, Vera S.
Baranova, Anna A.
Vasilchenko, Alexey S.
Lushpa, Vladislav A.
Mineev, Konstantin S.
Georgieva, Marina L.
Kul’ko, Alexander B.
Krasheninnikov, Mikhail E.
Lyundup, Alexey V.
Vasilchenko, Anastasia V.
Andreev, Yaroslav A.
author_facet Rogozhin, Eugene A.
Sadykova, Vera S.
Baranova, Anna A.
Vasilchenko, Alexey S.
Lushpa, Vladislav A.
Mineev, Konstantin S.
Georgieva, Marina L.
Kul’ko, Alexander B.
Krasheninnikov, Mikhail E.
Lyundup, Alexey V.
Vasilchenko, Anastasia V.
Andreev, Yaroslav A.
author_sort Rogozhin, Eugene A.
collection PubMed
description Soil fungi are known to contain a rich variety of defense metabolites that allow them to compete with other organisms (fungi, bacteria, nematodes, and insects) and help them occupy more preferential areas at the expense of effective antagonism. These compounds possess antibiotic activity towards a wide range of other microbes, particularly fungi that belong to different taxonomical units. These compounds include peptaibols, which are non-ribosomal synthesized polypeptides containing non-standard amino acid residues (alpha-aminoisobutyric acid mandatory) and some posttranslational modifications. We isolated a novel antibiotic peptide from the culture medium of Emericellopsis alkalina, an alkalophilic strain. This peptide, called emericellipsin A, exhibited a strong antifungal effect against the yeast Candida albicans, the mold fungus Aspergillus niger, and human pathogen clinical isolates. It also exhibited antimicrobial activity against some Gram-positive and Gram-negative bacteria. Additionally, emericellipsin A showed a significant cytotoxic effect and was highly active against Hep G2 and HeLa tumor cell lines. We used NMR spectroscopy to reveal that this peptaibol is nine amino acid residues long and contains non-standard amino acids. The mode of molecular action of emericellipsin A is most likely associated with its effects on the membranes of cells. Emericellipsin A is rather short peptaibol and could be useful for the development of antifungal, antibacterial, or anti-tumor remedies.
format Online
Article
Text
id pubmed-6278523
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62785232018-12-13 A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina Rogozhin, Eugene A. Sadykova, Vera S. Baranova, Anna A. Vasilchenko, Alexey S. Lushpa, Vladislav A. Mineev, Konstantin S. Georgieva, Marina L. Kul’ko, Alexander B. Krasheninnikov, Mikhail E. Lyundup, Alexey V. Vasilchenko, Anastasia V. Andreev, Yaroslav A. Molecules Article Soil fungi are known to contain a rich variety of defense metabolites that allow them to compete with other organisms (fungi, bacteria, nematodes, and insects) and help them occupy more preferential areas at the expense of effective antagonism. These compounds possess antibiotic activity towards a wide range of other microbes, particularly fungi that belong to different taxonomical units. These compounds include peptaibols, which are non-ribosomal synthesized polypeptides containing non-standard amino acid residues (alpha-aminoisobutyric acid mandatory) and some posttranslational modifications. We isolated a novel antibiotic peptide from the culture medium of Emericellopsis alkalina, an alkalophilic strain. This peptide, called emericellipsin A, exhibited a strong antifungal effect against the yeast Candida albicans, the mold fungus Aspergillus niger, and human pathogen clinical isolates. It also exhibited antimicrobial activity against some Gram-positive and Gram-negative bacteria. Additionally, emericellipsin A showed a significant cytotoxic effect and was highly active against Hep G2 and HeLa tumor cell lines. We used NMR spectroscopy to reveal that this peptaibol is nine amino acid residues long and contains non-standard amino acids. The mode of molecular action of emericellipsin A is most likely associated with its effects on the membranes of cells. Emericellipsin A is rather short peptaibol and could be useful for the development of antifungal, antibacterial, or anti-tumor remedies. MDPI 2018-10-27 /pmc/articles/PMC6278523/ /pubmed/30373232 http://dx.doi.org/10.3390/molecules23112785 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rogozhin, Eugene A.
Sadykova, Vera S.
Baranova, Anna A.
Vasilchenko, Alexey S.
Lushpa, Vladislav A.
Mineev, Konstantin S.
Georgieva, Marina L.
Kul’ko, Alexander B.
Krasheninnikov, Mikhail E.
Lyundup, Alexey V.
Vasilchenko, Anastasia V.
Andreev, Yaroslav A.
A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina
title A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina
title_full A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina
title_fullStr A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina
title_full_unstemmed A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina
title_short A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina
title_sort novel lipopeptaibol emericellipsin a with antimicrobial and antitumor activity produced by the extremophilic fungus emericellopsis alkalina
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278523/
https://www.ncbi.nlm.nih.gov/pubmed/30373232
http://dx.doi.org/10.3390/molecules23112785
work_keys_str_mv AT rogozhineugenea anovellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT sadykovaveras anovellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT baranovaannaa anovellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT vasilchenkoalexeys anovellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT lushpavladislava anovellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT mineevkonstantins anovellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT georgievamarinal anovellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT kulkoalexanderb anovellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT krasheninnikovmikhaile anovellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT lyundupalexeyv anovellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT vasilchenkoanastasiav anovellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT andreevyaroslava anovellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT rogozhineugenea novellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT sadykovaveras novellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT baranovaannaa novellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT vasilchenkoalexeys novellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT lushpavladislava novellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT mineevkonstantins novellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT georgievamarinal novellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT kulkoalexanderb novellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT krasheninnikovmikhaile novellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT lyundupalexeyv novellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT vasilchenkoanastasiav novellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina
AT andreevyaroslava novellipopeptaibolemericellipsinawithantimicrobialandantitumoractivityproducedbytheextremophilicfungusemericellopsisalkalina