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A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina
Soil fungi are known to contain a rich variety of defense metabolites that allow them to compete with other organisms (fungi, bacteria, nematodes, and insects) and help them occupy more preferential areas at the expense of effective antagonism. These compounds possess antibiotic activity towards a w...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278523/ https://www.ncbi.nlm.nih.gov/pubmed/30373232 http://dx.doi.org/10.3390/molecules23112785 |
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author | Rogozhin, Eugene A. Sadykova, Vera S. Baranova, Anna A. Vasilchenko, Alexey S. Lushpa, Vladislav A. Mineev, Konstantin S. Georgieva, Marina L. Kul’ko, Alexander B. Krasheninnikov, Mikhail E. Lyundup, Alexey V. Vasilchenko, Anastasia V. Andreev, Yaroslav A. |
author_facet | Rogozhin, Eugene A. Sadykova, Vera S. Baranova, Anna A. Vasilchenko, Alexey S. Lushpa, Vladislav A. Mineev, Konstantin S. Georgieva, Marina L. Kul’ko, Alexander B. Krasheninnikov, Mikhail E. Lyundup, Alexey V. Vasilchenko, Anastasia V. Andreev, Yaroslav A. |
author_sort | Rogozhin, Eugene A. |
collection | PubMed |
description | Soil fungi are known to contain a rich variety of defense metabolites that allow them to compete with other organisms (fungi, bacteria, nematodes, and insects) and help them occupy more preferential areas at the expense of effective antagonism. These compounds possess antibiotic activity towards a wide range of other microbes, particularly fungi that belong to different taxonomical units. These compounds include peptaibols, which are non-ribosomal synthesized polypeptides containing non-standard amino acid residues (alpha-aminoisobutyric acid mandatory) and some posttranslational modifications. We isolated a novel antibiotic peptide from the culture medium of Emericellopsis alkalina, an alkalophilic strain. This peptide, called emericellipsin A, exhibited a strong antifungal effect against the yeast Candida albicans, the mold fungus Aspergillus niger, and human pathogen clinical isolates. It also exhibited antimicrobial activity against some Gram-positive and Gram-negative bacteria. Additionally, emericellipsin A showed a significant cytotoxic effect and was highly active against Hep G2 and HeLa tumor cell lines. We used NMR spectroscopy to reveal that this peptaibol is nine amino acid residues long and contains non-standard amino acids. The mode of molecular action of emericellipsin A is most likely associated with its effects on the membranes of cells. Emericellipsin A is rather short peptaibol and could be useful for the development of antifungal, antibacterial, or anti-tumor remedies. |
format | Online Article Text |
id | pubmed-6278523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62785232018-12-13 A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina Rogozhin, Eugene A. Sadykova, Vera S. Baranova, Anna A. Vasilchenko, Alexey S. Lushpa, Vladislav A. Mineev, Konstantin S. Georgieva, Marina L. Kul’ko, Alexander B. Krasheninnikov, Mikhail E. Lyundup, Alexey V. Vasilchenko, Anastasia V. Andreev, Yaroslav A. Molecules Article Soil fungi are known to contain a rich variety of defense metabolites that allow them to compete with other organisms (fungi, bacteria, nematodes, and insects) and help them occupy more preferential areas at the expense of effective antagonism. These compounds possess antibiotic activity towards a wide range of other microbes, particularly fungi that belong to different taxonomical units. These compounds include peptaibols, which are non-ribosomal synthesized polypeptides containing non-standard amino acid residues (alpha-aminoisobutyric acid mandatory) and some posttranslational modifications. We isolated a novel antibiotic peptide from the culture medium of Emericellopsis alkalina, an alkalophilic strain. This peptide, called emericellipsin A, exhibited a strong antifungal effect against the yeast Candida albicans, the mold fungus Aspergillus niger, and human pathogen clinical isolates. It also exhibited antimicrobial activity against some Gram-positive and Gram-negative bacteria. Additionally, emericellipsin A showed a significant cytotoxic effect and was highly active against Hep G2 and HeLa tumor cell lines. We used NMR spectroscopy to reveal that this peptaibol is nine amino acid residues long and contains non-standard amino acids. The mode of molecular action of emericellipsin A is most likely associated with its effects on the membranes of cells. Emericellipsin A is rather short peptaibol and could be useful for the development of antifungal, antibacterial, or anti-tumor remedies. MDPI 2018-10-27 /pmc/articles/PMC6278523/ /pubmed/30373232 http://dx.doi.org/10.3390/molecules23112785 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rogozhin, Eugene A. Sadykova, Vera S. Baranova, Anna A. Vasilchenko, Alexey S. Lushpa, Vladislav A. Mineev, Konstantin S. Georgieva, Marina L. Kul’ko, Alexander B. Krasheninnikov, Mikhail E. Lyundup, Alexey V. Vasilchenko, Anastasia V. Andreev, Yaroslav A. A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina |
title | A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina |
title_full | A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina |
title_fullStr | A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina |
title_full_unstemmed | A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina |
title_short | A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina |
title_sort | novel lipopeptaibol emericellipsin a with antimicrobial and antitumor activity produced by the extremophilic fungus emericellopsis alkalina |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278523/ https://www.ncbi.nlm.nih.gov/pubmed/30373232 http://dx.doi.org/10.3390/molecules23112785 |
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