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Multicellular Human Gastric Cancer Spheroids Mimic the Glycosylation Phenotype of Gastric Carcinomas

Cellular glycosylation plays a pivotal role in several molecular mechanisms controlling cell–cell recognition, communication, and adhesion. Thus, aberrant glycosylation has a major impact on the acquisition of malignant features in the tumor progression of patients. To mimic these in vivo features,...

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Autores principales: Balmaña, Meritxell, Mereiter, Stefan, Diniz, Francisca, Feijão, Tália, Barrias, Cristina C., Reis, Celso A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278543/
https://www.ncbi.nlm.nih.gov/pubmed/30380716
http://dx.doi.org/10.3390/molecules23112815
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author Balmaña, Meritxell
Mereiter, Stefan
Diniz, Francisca
Feijão, Tália
Barrias, Cristina C.
Reis, Celso A.
author_facet Balmaña, Meritxell
Mereiter, Stefan
Diniz, Francisca
Feijão, Tália
Barrias, Cristina C.
Reis, Celso A.
author_sort Balmaña, Meritxell
collection PubMed
description Cellular glycosylation plays a pivotal role in several molecular mechanisms controlling cell–cell recognition, communication, and adhesion. Thus, aberrant glycosylation has a major impact on the acquisition of malignant features in the tumor progression of patients. To mimic these in vivo features, an innovative high-throughput 3D spheroid culture methodology has been developed for gastric cancer cells. The assessment of cancer cell spheroids’ physical characteristics, such as size, morphology and solidity, as well as the impact of glycosylation inhibitors on spheroid formation was performed applying automated image analysis. A detailed evaluation of key glycans and glycoproteins displayed by the gastric cancer spheroids and their counterpart cells cultured under conventional 2D conditions was performed. Our results show that, by applying 3D cell culture approaches, the model cell lines represented the differentiation features observed in the original tumors and the cellular glycocalix underwent striking changes, displaying increased expression of cancer-associated glycan antigens and mucin MUC1, ultimately better simulating the glycosylation phenotype of the gastric tumor.
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spelling pubmed-62785432018-12-13 Multicellular Human Gastric Cancer Spheroids Mimic the Glycosylation Phenotype of Gastric Carcinomas Balmaña, Meritxell Mereiter, Stefan Diniz, Francisca Feijão, Tália Barrias, Cristina C. Reis, Celso A. Molecules Article Cellular glycosylation plays a pivotal role in several molecular mechanisms controlling cell–cell recognition, communication, and adhesion. Thus, aberrant glycosylation has a major impact on the acquisition of malignant features in the tumor progression of patients. To mimic these in vivo features, an innovative high-throughput 3D spheroid culture methodology has been developed for gastric cancer cells. The assessment of cancer cell spheroids’ physical characteristics, such as size, morphology and solidity, as well as the impact of glycosylation inhibitors on spheroid formation was performed applying automated image analysis. A detailed evaluation of key glycans and glycoproteins displayed by the gastric cancer spheroids and their counterpart cells cultured under conventional 2D conditions was performed. Our results show that, by applying 3D cell culture approaches, the model cell lines represented the differentiation features observed in the original tumors and the cellular glycocalix underwent striking changes, displaying increased expression of cancer-associated glycan antigens and mucin MUC1, ultimately better simulating the glycosylation phenotype of the gastric tumor. MDPI 2018-10-30 /pmc/articles/PMC6278543/ /pubmed/30380716 http://dx.doi.org/10.3390/molecules23112815 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Balmaña, Meritxell
Mereiter, Stefan
Diniz, Francisca
Feijão, Tália
Barrias, Cristina C.
Reis, Celso A.
Multicellular Human Gastric Cancer Spheroids Mimic the Glycosylation Phenotype of Gastric Carcinomas
title Multicellular Human Gastric Cancer Spheroids Mimic the Glycosylation Phenotype of Gastric Carcinomas
title_full Multicellular Human Gastric Cancer Spheroids Mimic the Glycosylation Phenotype of Gastric Carcinomas
title_fullStr Multicellular Human Gastric Cancer Spheroids Mimic the Glycosylation Phenotype of Gastric Carcinomas
title_full_unstemmed Multicellular Human Gastric Cancer Spheroids Mimic the Glycosylation Phenotype of Gastric Carcinomas
title_short Multicellular Human Gastric Cancer Spheroids Mimic the Glycosylation Phenotype of Gastric Carcinomas
title_sort multicellular human gastric cancer spheroids mimic the glycosylation phenotype of gastric carcinomas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278543/
https://www.ncbi.nlm.nih.gov/pubmed/30380716
http://dx.doi.org/10.3390/molecules23112815
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