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Derivatization of Methylglyoxal for LC-ESI-MS Analysis—Stability and Relative Sensitivity of Different Derivatives

The great research interest in the quantification of reactive carbonyl compounds (RCCs), such as methylglyoxal (MGO) in biological and environmental samples, is reflected by the fact that several publications have described specific strategies to perform this task. Thus, many reagents have also been...

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Autores principales: Fritzsche, Stefan, Billig, Susan, Rynek, Robby, Abburi, Ramarao, Tarakhovskaya, Elena, Leuner, Olga, Frolov, Andrej, Birkemeyer, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278547/
https://www.ncbi.nlm.nih.gov/pubmed/30453519
http://dx.doi.org/10.3390/molecules23112994
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author Fritzsche, Stefan
Billig, Susan
Rynek, Robby
Abburi, Ramarao
Tarakhovskaya, Elena
Leuner, Olga
Frolov, Andrej
Birkemeyer, Claudia
author_facet Fritzsche, Stefan
Billig, Susan
Rynek, Robby
Abburi, Ramarao
Tarakhovskaya, Elena
Leuner, Olga
Frolov, Andrej
Birkemeyer, Claudia
author_sort Fritzsche, Stefan
collection PubMed
description The great research interest in the quantification of reactive carbonyl compounds (RCCs), such as methylglyoxal (MGO) in biological and environmental samples, is reflected by the fact that several publications have described specific strategies to perform this task. Thus, many reagents have also been reported for the derivatization of RCCs to effectively detect and quantify the resulting compounds using sensitive techniques such as liquid chromatography coupled with mass spectrometry (LC-MS). However, the choice of the derivatization protocol is not always clear, and a comparative evaluation is not feasible because detection limits from separate reports and determined with different instruments are hardly comparable. Consequently, for a systematic comparison, we tested 21 agents in one experimental setup for derivatization of RCCs prior to LC-MS analysis. This consisted of seven commonly employed reagents and 14 similar reagents, three of which were designed and synthesized by us. All reagents were probed for analytical responsiveness of the derivatives and stability of the reaction mixtures. The results showed that derivatives of 4-methoxyphenylenediamine and 3-methoxyphenylhydrazine—reported here for the first time for derivatization of RCCs—provided a particularly high responsiveness with ESI-MS detection. We applied the protocol to investigate MGO contamination of laboratory water and show successful quantification in a lipoxidation experiment. In summary, our results provide valuable information for scientists in establishing accurate analysis of RCCs.
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spelling pubmed-62785472018-12-13 Derivatization of Methylglyoxal for LC-ESI-MS Analysis—Stability and Relative Sensitivity of Different Derivatives Fritzsche, Stefan Billig, Susan Rynek, Robby Abburi, Ramarao Tarakhovskaya, Elena Leuner, Olga Frolov, Andrej Birkemeyer, Claudia Molecules Article The great research interest in the quantification of reactive carbonyl compounds (RCCs), such as methylglyoxal (MGO) in biological and environmental samples, is reflected by the fact that several publications have described specific strategies to perform this task. Thus, many reagents have also been reported for the derivatization of RCCs to effectively detect and quantify the resulting compounds using sensitive techniques such as liquid chromatography coupled with mass spectrometry (LC-MS). However, the choice of the derivatization protocol is not always clear, and a comparative evaluation is not feasible because detection limits from separate reports and determined with different instruments are hardly comparable. Consequently, for a systematic comparison, we tested 21 agents in one experimental setup for derivatization of RCCs prior to LC-MS analysis. This consisted of seven commonly employed reagents and 14 similar reagents, three of which were designed and synthesized by us. All reagents were probed for analytical responsiveness of the derivatives and stability of the reaction mixtures. The results showed that derivatives of 4-methoxyphenylenediamine and 3-methoxyphenylhydrazine—reported here for the first time for derivatization of RCCs—provided a particularly high responsiveness with ESI-MS detection. We applied the protocol to investigate MGO contamination of laboratory water and show successful quantification in a lipoxidation experiment. In summary, our results provide valuable information for scientists in establishing accurate analysis of RCCs. MDPI 2018-11-16 /pmc/articles/PMC6278547/ /pubmed/30453519 http://dx.doi.org/10.3390/molecules23112994 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fritzsche, Stefan
Billig, Susan
Rynek, Robby
Abburi, Ramarao
Tarakhovskaya, Elena
Leuner, Olga
Frolov, Andrej
Birkemeyer, Claudia
Derivatization of Methylglyoxal for LC-ESI-MS Analysis—Stability and Relative Sensitivity of Different Derivatives
title Derivatization of Methylglyoxal for LC-ESI-MS Analysis—Stability and Relative Sensitivity of Different Derivatives
title_full Derivatization of Methylglyoxal for LC-ESI-MS Analysis—Stability and Relative Sensitivity of Different Derivatives
title_fullStr Derivatization of Methylglyoxal for LC-ESI-MS Analysis—Stability and Relative Sensitivity of Different Derivatives
title_full_unstemmed Derivatization of Methylglyoxal for LC-ESI-MS Analysis—Stability and Relative Sensitivity of Different Derivatives
title_short Derivatization of Methylglyoxal for LC-ESI-MS Analysis—Stability and Relative Sensitivity of Different Derivatives
title_sort derivatization of methylglyoxal for lc-esi-ms analysis—stability and relative sensitivity of different derivatives
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278547/
https://www.ncbi.nlm.nih.gov/pubmed/30453519
http://dx.doi.org/10.3390/molecules23112994
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