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Tirucallane Triterpenoids from the Stems and Stem Bark of Cornus walteri that Control Adipocyte and Osteoblast Differentiations

Cornus walteri Wanger (Cornaceae) has been broadly used in traditional East Asian medicine for the treatment of various disorders, including skin inflammation and diarrhea. As part of our efforts to identify structurally and/or biologically new compounds from Korean medicinal plants, we have explore...

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Autores principales: Lee, Seoung Rak, Choi, Eunyong, Jeon, Se Hun, Zhi, Xue Yan, Yu, Jae Sik, Kim, Seon-Hee, Lee, Jeongmi, Park, Ki-Moon, Kim, Ki Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278563/
https://www.ncbi.nlm.nih.gov/pubmed/30360474
http://dx.doi.org/10.3390/molecules23112732
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author Lee, Seoung Rak
Choi, Eunyong
Jeon, Se Hun
Zhi, Xue Yan
Yu, Jae Sik
Kim, Seon-Hee
Lee, Jeongmi
Park, Ki-Moon
Kim, Ki Hyun
author_facet Lee, Seoung Rak
Choi, Eunyong
Jeon, Se Hun
Zhi, Xue Yan
Yu, Jae Sik
Kim, Seon-Hee
Lee, Jeongmi
Park, Ki-Moon
Kim, Ki Hyun
author_sort Lee, Seoung Rak
collection PubMed
description Cornus walteri Wanger (Cornaceae) has been broadly used in traditional East Asian medicine for the treatment of various disorders, including skin inflammation and diarrhea. As part of our efforts to identify structurally and/or biologically new compounds from Korean medicinal plants, we have explored potentially new bioactive constituents from C. walteri. In the present study, seven triterpenoids (1–7) were isolated from C. walteri stems and stem bark. Compounds 1–3 were new tirucallane triterpenoids (cornusalterins N-P) and compounds 4–7 were isolated for the first time from C. walteri. The structures of the new compounds were determined based on 1D and 2D NMR spectroscopic data interpretations and HR-ESIMS, as well as a computational method coupled with a statistical procedure (DP4+). The regulatory effects of the isolated triterpenoids (1–7) on mesenchymal stem cell (MSC) differentiation to adipocytes and osteoblasts were examined in the C3H10T1/2 cell line. Although these compounds had little effect on MSC differentiation to osteoblasts, lipid droplet formation in adipocyte-differentiated MSCs decreased in the presence of the seven triterpenoids. Compounds 1 and 4 each had a relatively distinct correlation between dose and efficacy, showing adipogenesis suppression at higher concentrations. Our findings demonstrate that the active compounds 1 and 4 can exert beneficial effects in regulation of adipocyte differentiation.
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spelling pubmed-62785632018-12-13 Tirucallane Triterpenoids from the Stems and Stem Bark of Cornus walteri that Control Adipocyte and Osteoblast Differentiations Lee, Seoung Rak Choi, Eunyong Jeon, Se Hun Zhi, Xue Yan Yu, Jae Sik Kim, Seon-Hee Lee, Jeongmi Park, Ki-Moon Kim, Ki Hyun Molecules Communication Cornus walteri Wanger (Cornaceae) has been broadly used in traditional East Asian medicine for the treatment of various disorders, including skin inflammation and diarrhea. As part of our efforts to identify structurally and/or biologically new compounds from Korean medicinal plants, we have explored potentially new bioactive constituents from C. walteri. In the present study, seven triterpenoids (1–7) were isolated from C. walteri stems and stem bark. Compounds 1–3 were new tirucallane triterpenoids (cornusalterins N-P) and compounds 4–7 were isolated for the first time from C. walteri. The structures of the new compounds were determined based on 1D and 2D NMR spectroscopic data interpretations and HR-ESIMS, as well as a computational method coupled with a statistical procedure (DP4+). The regulatory effects of the isolated triterpenoids (1–7) on mesenchymal stem cell (MSC) differentiation to adipocytes and osteoblasts were examined in the C3H10T1/2 cell line. Although these compounds had little effect on MSC differentiation to osteoblasts, lipid droplet formation in adipocyte-differentiated MSCs decreased in the presence of the seven triterpenoids. Compounds 1 and 4 each had a relatively distinct correlation between dose and efficacy, showing adipogenesis suppression at higher concentrations. Our findings demonstrate that the active compounds 1 and 4 can exert beneficial effects in regulation of adipocyte differentiation. MDPI 2018-10-23 /pmc/articles/PMC6278563/ /pubmed/30360474 http://dx.doi.org/10.3390/molecules23112732 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Lee, Seoung Rak
Choi, Eunyong
Jeon, Se Hun
Zhi, Xue Yan
Yu, Jae Sik
Kim, Seon-Hee
Lee, Jeongmi
Park, Ki-Moon
Kim, Ki Hyun
Tirucallane Triterpenoids from the Stems and Stem Bark of Cornus walteri that Control Adipocyte and Osteoblast Differentiations
title Tirucallane Triterpenoids from the Stems and Stem Bark of Cornus walteri that Control Adipocyte and Osteoblast Differentiations
title_full Tirucallane Triterpenoids from the Stems and Stem Bark of Cornus walteri that Control Adipocyte and Osteoblast Differentiations
title_fullStr Tirucallane Triterpenoids from the Stems and Stem Bark of Cornus walteri that Control Adipocyte and Osteoblast Differentiations
title_full_unstemmed Tirucallane Triterpenoids from the Stems and Stem Bark of Cornus walteri that Control Adipocyte and Osteoblast Differentiations
title_short Tirucallane Triterpenoids from the Stems and Stem Bark of Cornus walteri that Control Adipocyte and Osteoblast Differentiations
title_sort tirucallane triterpenoids from the stems and stem bark of cornus walteri that control adipocyte and osteoblast differentiations
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278563/
https://www.ncbi.nlm.nih.gov/pubmed/30360474
http://dx.doi.org/10.3390/molecules23112732
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