Cargando…
Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation
Clathrin light chains (CLCs) control selective uptake of a range of G protein–coupled receptors (GPCRs), although the mechanism by which this occurs has remained elusive thus far. In particular, site-specific phosphorylation of CLCb controls the uptake of the purinergic GPCR P2Y(12), but it is dispe...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6279376/ https://www.ncbi.nlm.nih.gov/pubmed/30228161 http://dx.doi.org/10.1083/jcb.201805005 |
_version_ | 1783378493399105536 |
---|---|
author | Maib, Hannes Ferreira, Filipe Vassilopoulos, Stéphane Smythe, Elizabeth |
author_facet | Maib, Hannes Ferreira, Filipe Vassilopoulos, Stéphane Smythe, Elizabeth |
author_sort | Maib, Hannes |
collection | PubMed |
description | Clathrin light chains (CLCs) control selective uptake of a range of G protein–coupled receptors (GPCRs), although the mechanism by which this occurs has remained elusive thus far. In particular, site-specific phosphorylation of CLCb controls the uptake of the purinergic GPCR P2Y(12), but it is dispensable for the constitutive uptake of the transferrin receptor (TfR). We demonstrate that phosphorylation of CLCb is required for the maturation of clathrin-coated pits (CCPs) through the transition of flat lattices into invaginated buds. This transition is dependent on efficient clathrin exchange regulated by CLCb phosphorylation and mediated through auxilin. Strikingly, this rearrangement is required for the uptake of P2Y(12) but not TfR. These findings link auxilin-mediated clathrin exchange to early stages of CCP invagination in a cargo-specific manner. This supports a model in which CCPs invaginate with variable modes of curvature depending on the cargo they incorporate. |
format | Online Article Text |
id | pubmed-6279376 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-62793762018-12-06 Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation Maib, Hannes Ferreira, Filipe Vassilopoulos, Stéphane Smythe, Elizabeth J Cell Biol Research Articles Clathrin light chains (CLCs) control selective uptake of a range of G protein–coupled receptors (GPCRs), although the mechanism by which this occurs has remained elusive thus far. In particular, site-specific phosphorylation of CLCb controls the uptake of the purinergic GPCR P2Y(12), but it is dispensable for the constitutive uptake of the transferrin receptor (TfR). We demonstrate that phosphorylation of CLCb is required for the maturation of clathrin-coated pits (CCPs) through the transition of flat lattices into invaginated buds. This transition is dependent on efficient clathrin exchange regulated by CLCb phosphorylation and mediated through auxilin. Strikingly, this rearrangement is required for the uptake of P2Y(12) but not TfR. These findings link auxilin-mediated clathrin exchange to early stages of CCP invagination in a cargo-specific manner. This supports a model in which CCPs invaginate with variable modes of curvature depending on the cargo they incorporate. Rockefeller University Press 2018-12-03 /pmc/articles/PMC6279376/ /pubmed/30228161 http://dx.doi.org/10.1083/jcb.201805005 Text en © 2018 Maib et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Maib, Hannes Ferreira, Filipe Vassilopoulos, Stéphane Smythe, Elizabeth Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation |
title | Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation |
title_full | Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation |
title_fullStr | Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation |
title_full_unstemmed | Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation |
title_short | Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation |
title_sort | cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6279376/ https://www.ncbi.nlm.nih.gov/pubmed/30228161 http://dx.doi.org/10.1083/jcb.201805005 |
work_keys_str_mv | AT maibhannes cargoregulatesclathrincoatedpitinvaginationviaclathrinlightchainphosphorylation AT ferreirafilipe cargoregulatesclathrincoatedpitinvaginationviaclathrinlightchainphosphorylation AT vassilopoulosstephane cargoregulatesclathrincoatedpitinvaginationviaclathrinlightchainphosphorylation AT smytheelizabeth cargoregulatesclathrincoatedpitinvaginationviaclathrinlightchainphosphorylation |