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Identification and anti-cancer activity in 2D and 3D cell culture evaluation of an Iranian isolated marine microalgae Picochlorum sp. RCC486

PURPOSE: Cancer disease is the second cause of death in the world. Now a days, high percentage of drugs, which are involved in treatment of cancers, have natural origin. Introduction of microalgae strains as anti-cancer drugs origin is a valuable approach for cancer therapy. METHODS: In the present...

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Autores principales: Abolhasani, Mohammad Haji, Safavi, Maliheh, Goodarzi, Mohammad Taghi, Kassaee, Seyed Mehrdad, Azin, Mehrdad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6279668/
https://www.ncbi.nlm.nih.gov/pubmed/30242672
http://dx.doi.org/10.1007/s40199-018-0213-5
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author Abolhasani, Mohammad Haji
Safavi, Maliheh
Goodarzi, Mohammad Taghi
Kassaee, Seyed Mehrdad
Azin, Mehrdad
author_facet Abolhasani, Mohammad Haji
Safavi, Maliheh
Goodarzi, Mohammad Taghi
Kassaee, Seyed Mehrdad
Azin, Mehrdad
author_sort Abolhasani, Mohammad Haji
collection PubMed
description PURPOSE: Cancer disease is the second cause of death in the world. Now a days, high percentage of drugs, which are involved in treatment of cancers, have natural origin. Introduction of microalgae strains as anti-cancer drugs origin is a valuable approach for cancer therapy. METHODS: In the present study we describe the isolation, characterization, and anti-proliferative activity of a new microalga strain (Picochlorum sp. RCC486) from Iran. The cytotoxic activity of four different algal extracts including methanol, ethyl acetate, chloroform, and hexane were evaluated against MDA-MB-231, MCF-7, Hep-G2, and A-549 cell liens. Cell viability was determined using MTT assay in both monolayer and spheroids 3D cultures. The apoptosis was confirmed by different methods such as AO/EB and Annexin V-FITC/PI double staining, caspase-3 colorimetric assay, ROS and MMP assay. RESULTS: The results of MTT assay and fluorescent double staining confirmed that methanol and ethyl acetate extracts showed the best cytotoxic activity against the cancer cell lines. The production of ROS, caspase-3 activity and depolarized MMP were quite significant in MDA-MB-231 cell line treated with methanol and ethyl acetate extracts. CONCLUSION: In this research we revealed that cytotoxicity and apoptotic effects of the methanol and ethyl acetate extracts in human cancer cells make them good candidates for further pharmacological studies to discover effective drugs for cancer therapy. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s40199-018-0213-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-62796682019-09-21 Identification and anti-cancer activity in 2D and 3D cell culture evaluation of an Iranian isolated marine microalgae Picochlorum sp. RCC486 Abolhasani, Mohammad Haji Safavi, Maliheh Goodarzi, Mohammad Taghi Kassaee, Seyed Mehrdad Azin, Mehrdad Daru Research Article PURPOSE: Cancer disease is the second cause of death in the world. Now a days, high percentage of drugs, which are involved in treatment of cancers, have natural origin. Introduction of microalgae strains as anti-cancer drugs origin is a valuable approach for cancer therapy. METHODS: In the present study we describe the isolation, characterization, and anti-proliferative activity of a new microalga strain (Picochlorum sp. RCC486) from Iran. The cytotoxic activity of four different algal extracts including methanol, ethyl acetate, chloroform, and hexane were evaluated against MDA-MB-231, MCF-7, Hep-G2, and A-549 cell liens. Cell viability was determined using MTT assay in both monolayer and spheroids 3D cultures. The apoptosis was confirmed by different methods such as AO/EB and Annexin V-FITC/PI double staining, caspase-3 colorimetric assay, ROS and MMP assay. RESULTS: The results of MTT assay and fluorescent double staining confirmed that methanol and ethyl acetate extracts showed the best cytotoxic activity against the cancer cell lines. The production of ROS, caspase-3 activity and depolarized MMP were quite significant in MDA-MB-231 cell line treated with methanol and ethyl acetate extracts. CONCLUSION: In this research we revealed that cytotoxicity and apoptotic effects of the methanol and ethyl acetate extracts in human cancer cells make them good candidates for further pharmacological studies to discover effective drugs for cancer therapy. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s40199-018-0213-5) contains supplementary material, which is available to authorized users. Springer International Publishing 2018-09-21 /pmc/articles/PMC6279668/ /pubmed/30242672 http://dx.doi.org/10.1007/s40199-018-0213-5 Text en © Springer Nature Switzerland AG 2018
spellingShingle Research Article
Abolhasani, Mohammad Haji
Safavi, Maliheh
Goodarzi, Mohammad Taghi
Kassaee, Seyed Mehrdad
Azin, Mehrdad
Identification and anti-cancer activity in 2D and 3D cell culture evaluation of an Iranian isolated marine microalgae Picochlorum sp. RCC486
title Identification and anti-cancer activity in 2D and 3D cell culture evaluation of an Iranian isolated marine microalgae Picochlorum sp. RCC486
title_full Identification and anti-cancer activity in 2D and 3D cell culture evaluation of an Iranian isolated marine microalgae Picochlorum sp. RCC486
title_fullStr Identification and anti-cancer activity in 2D and 3D cell culture evaluation of an Iranian isolated marine microalgae Picochlorum sp. RCC486
title_full_unstemmed Identification and anti-cancer activity in 2D and 3D cell culture evaluation of an Iranian isolated marine microalgae Picochlorum sp. RCC486
title_short Identification and anti-cancer activity in 2D and 3D cell culture evaluation of an Iranian isolated marine microalgae Picochlorum sp. RCC486
title_sort identification and anti-cancer activity in 2d and 3d cell culture evaluation of an iranian isolated marine microalgae picochlorum sp. rcc486
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6279668/
https://www.ncbi.nlm.nih.gov/pubmed/30242672
http://dx.doi.org/10.1007/s40199-018-0213-5
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