Cargando…
Interactions between Roseburia intestinalis and diet modulate atherogenesis in a murine model
Humans with metabolic and inflammatory diseases frequently harbor lower levels of butyrate-producing bacteria in their gut. However, it is not known whether variation in the levels of these organisms is causally linked with disease development and whether diet modifies the impact of these bacteria o...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6280189/ https://www.ncbi.nlm.nih.gov/pubmed/30397344 http://dx.doi.org/10.1038/s41564-018-0272-x |
_version_ | 1783378618713374720 |
---|---|
author | Kasahara, Kazuyuki Krautkramer, Kimberly A. Org, Elin Romano, Kymberleigh A. Kerby, Robert L. Vivas, Eugenio I. Mehrabian, Margarete Denu, John M. Bäckhed, Fredrik Lusis, Aldons J. Rey, Federico E. |
author_facet | Kasahara, Kazuyuki Krautkramer, Kimberly A. Org, Elin Romano, Kymberleigh A. Kerby, Robert L. Vivas, Eugenio I. Mehrabian, Margarete Denu, John M. Bäckhed, Fredrik Lusis, Aldons J. Rey, Federico E. |
author_sort | Kasahara, Kazuyuki |
collection | PubMed |
description | Humans with metabolic and inflammatory diseases frequently harbor lower levels of butyrate-producing bacteria in their gut. However, it is not known whether variation in the levels of these organisms is causally linked with disease development and whether diet modifies the impact of these bacteria on health. Here we show that prominent gut-associated butyrate-producing bacteria species (Roseburia sp.) is inversely correlated with atherosclerotic lesion development in a genetically diverse mouse population. We use germ-free apolipoprotein E-deficient mice colonized with synthetic microbial communities that differ in their capacity to generate butyrate to demonstrate that Roseburia intestinalis interacts with dietary plant polysaccharides to (i) impact gene expression in the intestine, directing metabolism away from glycolysis and toward fatty acid utilization, (ii) lower systemic inflammation and (iii) ameliorate atherosclerosis. Furthermore, intestinal administration of butyrate reduces endotoxemia and atherosclerosis development. Altogether, our results illustrate how modifiable diet-by-microbiota interactions impact cardiovascular disease, and suggest that interventions aimed at increasing the representation of butyrate-producing bacteria may provide protection against atherosclerosis. |
format | Online Article Text |
id | pubmed-6280189 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
record_format | MEDLINE/PubMed |
spelling | pubmed-62801892019-05-05 Interactions between Roseburia intestinalis and diet modulate atherogenesis in a murine model Kasahara, Kazuyuki Krautkramer, Kimberly A. Org, Elin Romano, Kymberleigh A. Kerby, Robert L. Vivas, Eugenio I. Mehrabian, Margarete Denu, John M. Bäckhed, Fredrik Lusis, Aldons J. Rey, Federico E. Nat Microbiol Article Humans with metabolic and inflammatory diseases frequently harbor lower levels of butyrate-producing bacteria in their gut. However, it is not known whether variation in the levels of these organisms is causally linked with disease development and whether diet modifies the impact of these bacteria on health. Here we show that prominent gut-associated butyrate-producing bacteria species (Roseburia sp.) is inversely correlated with atherosclerotic lesion development in a genetically diverse mouse population. We use germ-free apolipoprotein E-deficient mice colonized with synthetic microbial communities that differ in their capacity to generate butyrate to demonstrate that Roseburia intestinalis interacts with dietary plant polysaccharides to (i) impact gene expression in the intestine, directing metabolism away from glycolysis and toward fatty acid utilization, (ii) lower systemic inflammation and (iii) ameliorate atherosclerosis. Furthermore, intestinal administration of butyrate reduces endotoxemia and atherosclerosis development. Altogether, our results illustrate how modifiable diet-by-microbiota interactions impact cardiovascular disease, and suggest that interventions aimed at increasing the representation of butyrate-producing bacteria may provide protection against atherosclerosis. 2018-11-05 2018-12 /pmc/articles/PMC6280189/ /pubmed/30397344 http://dx.doi.org/10.1038/s41564-018-0272-x Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Kasahara, Kazuyuki Krautkramer, Kimberly A. Org, Elin Romano, Kymberleigh A. Kerby, Robert L. Vivas, Eugenio I. Mehrabian, Margarete Denu, John M. Bäckhed, Fredrik Lusis, Aldons J. Rey, Federico E. Interactions between Roseburia intestinalis and diet modulate atherogenesis in a murine model |
title | Interactions between Roseburia intestinalis and diet modulate atherogenesis in a murine model |
title_full | Interactions between Roseburia intestinalis and diet modulate atherogenesis in a murine model |
title_fullStr | Interactions between Roseburia intestinalis and diet modulate atherogenesis in a murine model |
title_full_unstemmed | Interactions between Roseburia intestinalis and diet modulate atherogenesis in a murine model |
title_short | Interactions between Roseburia intestinalis and diet modulate atherogenesis in a murine model |
title_sort | interactions between roseburia intestinalis and diet modulate atherogenesis in a murine model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6280189/ https://www.ncbi.nlm.nih.gov/pubmed/30397344 http://dx.doi.org/10.1038/s41564-018-0272-x |
work_keys_str_mv | AT kasaharakazuyuki interactionsbetweenroseburiaintestinalisanddietmodulateatherogenesisinamurinemodel AT krautkramerkimberlya interactionsbetweenroseburiaintestinalisanddietmodulateatherogenesisinamurinemodel AT orgelin interactionsbetweenroseburiaintestinalisanddietmodulateatherogenesisinamurinemodel AT romanokymberleigha interactionsbetweenroseburiaintestinalisanddietmodulateatherogenesisinamurinemodel AT kerbyrobertl interactionsbetweenroseburiaintestinalisanddietmodulateatherogenesisinamurinemodel AT vivaseugenioi interactionsbetweenroseburiaintestinalisanddietmodulateatherogenesisinamurinemodel AT mehrabianmargarete interactionsbetweenroseburiaintestinalisanddietmodulateatherogenesisinamurinemodel AT denujohnm interactionsbetweenroseburiaintestinalisanddietmodulateatherogenesisinamurinemodel AT backhedfredrik interactionsbetweenroseburiaintestinalisanddietmodulateatherogenesisinamurinemodel AT lusisaldonsj interactionsbetweenroseburiaintestinalisanddietmodulateatherogenesisinamurinemodel AT reyfedericoe interactionsbetweenroseburiaintestinalisanddietmodulateatherogenesisinamurinemodel |