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Benchmarking in vitro tissue-engineered blood–brain barrier models
The blood–brain barrier (BBB) plays a key role in regulating transport into and out of the brain. With increasing interest in the role of the BBB in health and disease, there have been significant advances in the development of in vitro models. The value of these models to the research community is...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6280508/ https://www.ncbi.nlm.nih.gov/pubmed/30514389 http://dx.doi.org/10.1186/s12987-018-0117-2 |
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author | DeStefano, Jackson G. Jamieson, John J. Linville, Raleigh M. Searson, Peter C. |
author_facet | DeStefano, Jackson G. Jamieson, John J. Linville, Raleigh M. Searson, Peter C. |
author_sort | DeStefano, Jackson G. |
collection | PubMed |
description | The blood–brain barrier (BBB) plays a key role in regulating transport into and out of the brain. With increasing interest in the role of the BBB in health and disease, there have been significant advances in the development of in vitro models. The value of these models to the research community is critically dependent on recapitulating characteristics of the BBB in humans or animal models. However, benchmarking in vitro models is surprisingly difficult since much of our knowledge of the structure and function of the BBB comes from in vitro studies. Here we describe a set of parameters that we consider a starting point for benchmarking and validation. These parameters are associated with structure (ultrastructure, wall shear stress, geometry), microenvironment (basement membrane and extracellular matrix), barrier function (transendothelial electrical resistance, permeability, efflux transport), cell function (expression of BBB markers, turnover), and co-culture with other cell types (astrocytes and pericytes). In suggesting benchmarks, we rely primarily on imaging or direct measurements in humans and animal models. |
format | Online Article Text |
id | pubmed-6280508 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-62805082018-12-10 Benchmarking in vitro tissue-engineered blood–brain barrier models DeStefano, Jackson G. Jamieson, John J. Linville, Raleigh M. Searson, Peter C. Fluids Barriers CNS Review The blood–brain barrier (BBB) plays a key role in regulating transport into and out of the brain. With increasing interest in the role of the BBB in health and disease, there have been significant advances in the development of in vitro models. The value of these models to the research community is critically dependent on recapitulating characteristics of the BBB in humans or animal models. However, benchmarking in vitro models is surprisingly difficult since much of our knowledge of the structure and function of the BBB comes from in vitro studies. Here we describe a set of parameters that we consider a starting point for benchmarking and validation. These parameters are associated with structure (ultrastructure, wall shear stress, geometry), microenvironment (basement membrane and extracellular matrix), barrier function (transendothelial electrical resistance, permeability, efflux transport), cell function (expression of BBB markers, turnover), and co-culture with other cell types (astrocytes and pericytes). In suggesting benchmarks, we rely primarily on imaging or direct measurements in humans and animal models. BioMed Central 2018-12-04 /pmc/articles/PMC6280508/ /pubmed/30514389 http://dx.doi.org/10.1186/s12987-018-0117-2 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Review DeStefano, Jackson G. Jamieson, John J. Linville, Raleigh M. Searson, Peter C. Benchmarking in vitro tissue-engineered blood–brain barrier models |
title | Benchmarking in vitro tissue-engineered blood–brain barrier models |
title_full | Benchmarking in vitro tissue-engineered blood–brain barrier models |
title_fullStr | Benchmarking in vitro tissue-engineered blood–brain barrier models |
title_full_unstemmed | Benchmarking in vitro tissue-engineered blood–brain barrier models |
title_short | Benchmarking in vitro tissue-engineered blood–brain barrier models |
title_sort | benchmarking in vitro tissue-engineered blood–brain barrier models |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6280508/ https://www.ncbi.nlm.nih.gov/pubmed/30514389 http://dx.doi.org/10.1186/s12987-018-0117-2 |
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