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The Micronemal Plasmodium Proteins P36 and P52 Act in Concert to Establish the Replication-Permissive Compartment Within Infected Hepatocytes

Within the liver, Plasmodium sporozoites traverse cells searching for a “suitable” hepatocyte, invading these cells through a process that results in the formation of a parasitophorous vacuole (PV), within which the parasite undergoes intracellular replication as a liver stage. It was previously est...

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Autores principales: Arredondo, Silvia A., Swearingen, Kristian E., Martinson, Thomas, Steel, Ryan, Dankwa, Dorender A., Harupa, Anke, Camargo, Nelly, Betz, William, Vigdorovich, Vladimir, Oliver, Brian G., Kangwanrangsan, Niwat, Ishino, Tomoko, Sather, Noah, Mikolajczak, Sebastian, Vaughan, Ashley M., Torii, Motomi, Moritz, Robert L., Kappe, Stefan H. I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6280682/
https://www.ncbi.nlm.nih.gov/pubmed/30547015
http://dx.doi.org/10.3389/fcimb.2018.00413
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author Arredondo, Silvia A.
Swearingen, Kristian E.
Martinson, Thomas
Steel, Ryan
Dankwa, Dorender A.
Harupa, Anke
Camargo, Nelly
Betz, William
Vigdorovich, Vladimir
Oliver, Brian G.
Kangwanrangsan, Niwat
Ishino, Tomoko
Sather, Noah
Mikolajczak, Sebastian
Vaughan, Ashley M.
Torii, Motomi
Moritz, Robert L.
Kappe, Stefan H. I.
author_facet Arredondo, Silvia A.
Swearingen, Kristian E.
Martinson, Thomas
Steel, Ryan
Dankwa, Dorender A.
Harupa, Anke
Camargo, Nelly
Betz, William
Vigdorovich, Vladimir
Oliver, Brian G.
Kangwanrangsan, Niwat
Ishino, Tomoko
Sather, Noah
Mikolajczak, Sebastian
Vaughan, Ashley M.
Torii, Motomi
Moritz, Robert L.
Kappe, Stefan H. I.
author_sort Arredondo, Silvia A.
collection PubMed
description Within the liver, Plasmodium sporozoites traverse cells searching for a “suitable” hepatocyte, invading these cells through a process that results in the formation of a parasitophorous vacuole (PV), within which the parasite undergoes intracellular replication as a liver stage. It was previously established that two members of the Plasmodium s48/45 protein family, P36 and P52, are essential for productive invasion of host hepatocytes by sporozoites as their simultaneous deletion results in growth-arrested parasites that lack a PV. Recent studies point toward a pathway of entry possibly involving the interaction of P36 with hepatocyte receptors EphA2, CD81, and SR-B1. However, the relationship between P36 and P52 during sporozoite invasion remains unknown. Here we show that parasites with a single P52 or P36 gene deletion each lack a PV after hepatocyte invasion, thereby pheno-copying the lack of a PV observed for the P52/P36 dual gene deletion parasite line. This indicates that both proteins are equally important in the establishment of a PV and act in the same pathway. We created a Plasmodium yoelii P36(mCherry) tagged parasite line that allowed us to visualize the subcellular localization of P36 and found that it partially co-localizes with P52 in the sporozoite secretory microneme organelles. Furthermore, through co-immunoprecipitation studies in vivo, we determined that P36 and P52 form a protein complex in sporozoites, indicating a concerted function for both proteins within the PV formation pathway. However, upon sporozoite stimulation, only P36 was released as a secreted protein while P52 was not. Our results support a model in which the putatively glycosylphosphatidylinositol (GPI)-anchored P52 may serve as a scaffold to facilitate the interaction of secreted P36 with the host cell during sporozoite invasion of hepatocytes.
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spelling pubmed-62806822018-12-13 The Micronemal Plasmodium Proteins P36 and P52 Act in Concert to Establish the Replication-Permissive Compartment Within Infected Hepatocytes Arredondo, Silvia A. Swearingen, Kristian E. Martinson, Thomas Steel, Ryan Dankwa, Dorender A. Harupa, Anke Camargo, Nelly Betz, William Vigdorovich, Vladimir Oliver, Brian G. Kangwanrangsan, Niwat Ishino, Tomoko Sather, Noah Mikolajczak, Sebastian Vaughan, Ashley M. Torii, Motomi Moritz, Robert L. Kappe, Stefan H. I. Front Cell Infect Microbiol Cellular and Infection Microbiology Within the liver, Plasmodium sporozoites traverse cells searching for a “suitable” hepatocyte, invading these cells through a process that results in the formation of a parasitophorous vacuole (PV), within which the parasite undergoes intracellular replication as a liver stage. It was previously established that two members of the Plasmodium s48/45 protein family, P36 and P52, are essential for productive invasion of host hepatocytes by sporozoites as their simultaneous deletion results in growth-arrested parasites that lack a PV. Recent studies point toward a pathway of entry possibly involving the interaction of P36 with hepatocyte receptors EphA2, CD81, and SR-B1. However, the relationship between P36 and P52 during sporozoite invasion remains unknown. Here we show that parasites with a single P52 or P36 gene deletion each lack a PV after hepatocyte invasion, thereby pheno-copying the lack of a PV observed for the P52/P36 dual gene deletion parasite line. This indicates that both proteins are equally important in the establishment of a PV and act in the same pathway. We created a Plasmodium yoelii P36(mCherry) tagged parasite line that allowed us to visualize the subcellular localization of P36 and found that it partially co-localizes with P52 in the sporozoite secretory microneme organelles. Furthermore, through co-immunoprecipitation studies in vivo, we determined that P36 and P52 form a protein complex in sporozoites, indicating a concerted function for both proteins within the PV formation pathway. However, upon sporozoite stimulation, only P36 was released as a secreted protein while P52 was not. Our results support a model in which the putatively glycosylphosphatidylinositol (GPI)-anchored P52 may serve as a scaffold to facilitate the interaction of secreted P36 with the host cell during sporozoite invasion of hepatocytes. Frontiers Media S.A. 2018-11-27 /pmc/articles/PMC6280682/ /pubmed/30547015 http://dx.doi.org/10.3389/fcimb.2018.00413 Text en Copyright © 2018 Arredondo, Swearingen, Martinson, Steel, Dankwa, Harupa, Camargo, Betz, Vigdorovich, Oliver, Kangwanrangsan, Ishino, Sather, Mikolajczak, Vaughan, Torii, Moritz and Kappe. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Arredondo, Silvia A.
Swearingen, Kristian E.
Martinson, Thomas
Steel, Ryan
Dankwa, Dorender A.
Harupa, Anke
Camargo, Nelly
Betz, William
Vigdorovich, Vladimir
Oliver, Brian G.
Kangwanrangsan, Niwat
Ishino, Tomoko
Sather, Noah
Mikolajczak, Sebastian
Vaughan, Ashley M.
Torii, Motomi
Moritz, Robert L.
Kappe, Stefan H. I.
The Micronemal Plasmodium Proteins P36 and P52 Act in Concert to Establish the Replication-Permissive Compartment Within Infected Hepatocytes
title The Micronemal Plasmodium Proteins P36 and P52 Act in Concert to Establish the Replication-Permissive Compartment Within Infected Hepatocytes
title_full The Micronemal Plasmodium Proteins P36 and P52 Act in Concert to Establish the Replication-Permissive Compartment Within Infected Hepatocytes
title_fullStr The Micronemal Plasmodium Proteins P36 and P52 Act in Concert to Establish the Replication-Permissive Compartment Within Infected Hepatocytes
title_full_unstemmed The Micronemal Plasmodium Proteins P36 and P52 Act in Concert to Establish the Replication-Permissive Compartment Within Infected Hepatocytes
title_short The Micronemal Plasmodium Proteins P36 and P52 Act in Concert to Establish the Replication-Permissive Compartment Within Infected Hepatocytes
title_sort micronemal plasmodium proteins p36 and p52 act in concert to establish the replication-permissive compartment within infected hepatocytes
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6280682/
https://www.ncbi.nlm.nih.gov/pubmed/30547015
http://dx.doi.org/10.3389/fcimb.2018.00413
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