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Increased level of compleasomes in cerebrospinal fluid of patients with herpes simplex encephalitis

Herpes simplex encephalitis (HSE) is a common cause of viral encephalitis (HSV-1) characterised by pronounced inflammation and elevated intracranial pressure. We have shown in a rat model that HSV-1 infection causes an interaction between complement factors and proteasomes, leading to formation of p...

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Autores principales: Johansson, Ewa, Lange, Stefan, Bergström, Tomas, Oshalim, Merna, Lönnroth, Ivar, Studahl, Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6280959/
https://www.ncbi.nlm.nih.gov/pubmed/30094629
http://dx.doi.org/10.1007/s13365-018-0665-x
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author Johansson, Ewa
Lange, Stefan
Bergström, Tomas
Oshalim, Merna
Lönnroth, Ivar
Studahl, Marie
author_facet Johansson, Ewa
Lange, Stefan
Bergström, Tomas
Oshalim, Merna
Lönnroth, Ivar
Studahl, Marie
author_sort Johansson, Ewa
collection PubMed
description Herpes simplex encephalitis (HSE) is a common cause of viral encephalitis (HSV-1) characterised by pronounced inflammation and elevated intracranial pressure. We have shown in a rat model that HSV-1 infection causes an interaction between complement factors and proteasomes, leading to formation of proteasome/complement complexes (compleasomes). Exposure of the proteasome regulatory subunit antisecretory factor 1 (AF1) leads to a decrease in intracranial pressure. The aim of this study was to evaluate the acute and prolonged formation of compleasomes in cerebrospinal fluid (CSF) from patients with HSE. Cerebrospinal fluid samples (n = 55) from 24 HSE patients were analysed for compleasome complexes. Samples from healthy controls (n = 23) and patient controls (n = 27) served as baseline information. Sandwich enzyme-linked immunosorbent assay (ELISA) for proteasomes and their complex formation with complement factor 3 or 4, and Western blot for C3 activation were performed on CSF samples. Increased compleasome formation, both presenting as an initial formation and showing exposure of subunit AF1 in the compleasomes, was found in CSF samples drawn from patients with HSE compared with samples from the control groups (p < 0.0005). The total protein CSF concentration was equal in all groups. The levels were higher in the acute phase compared with late in the disease course (p < 0.0005). Complement 3 breakdown product iC3b was detected in CSF samples of the HSE patients. The early increased formation of compleasomes in CSF suggests that this complex may be involved in host defence against HSE.
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spelling pubmed-62809592018-12-26 Increased level of compleasomes in cerebrospinal fluid of patients with herpes simplex encephalitis Johansson, Ewa Lange, Stefan Bergström, Tomas Oshalim, Merna Lönnroth, Ivar Studahl, Marie J Neurovirol Article Herpes simplex encephalitis (HSE) is a common cause of viral encephalitis (HSV-1) characterised by pronounced inflammation and elevated intracranial pressure. We have shown in a rat model that HSV-1 infection causes an interaction between complement factors and proteasomes, leading to formation of proteasome/complement complexes (compleasomes). Exposure of the proteasome regulatory subunit antisecretory factor 1 (AF1) leads to a decrease in intracranial pressure. The aim of this study was to evaluate the acute and prolonged formation of compleasomes in cerebrospinal fluid (CSF) from patients with HSE. Cerebrospinal fluid samples (n = 55) from 24 HSE patients were analysed for compleasome complexes. Samples from healthy controls (n = 23) and patient controls (n = 27) served as baseline information. Sandwich enzyme-linked immunosorbent assay (ELISA) for proteasomes and their complex formation with complement factor 3 or 4, and Western blot for C3 activation were performed on CSF samples. Increased compleasome formation, both presenting as an initial formation and showing exposure of subunit AF1 in the compleasomes, was found in CSF samples drawn from patients with HSE compared with samples from the control groups (p < 0.0005). The total protein CSF concentration was equal in all groups. The levels were higher in the acute phase compared with late in the disease course (p < 0.0005). Complement 3 breakdown product iC3b was detected in CSF samples of the HSE patients. The early increased formation of compleasomes in CSF suggests that this complex may be involved in host defence against HSE. Springer International Publishing 2018-08-09 2018 /pmc/articles/PMC6280959/ /pubmed/30094629 http://dx.doi.org/10.1007/s13365-018-0665-x Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Johansson, Ewa
Lange, Stefan
Bergström, Tomas
Oshalim, Merna
Lönnroth, Ivar
Studahl, Marie
Increased level of compleasomes in cerebrospinal fluid of patients with herpes simplex encephalitis
title Increased level of compleasomes in cerebrospinal fluid of patients with herpes simplex encephalitis
title_full Increased level of compleasomes in cerebrospinal fluid of patients with herpes simplex encephalitis
title_fullStr Increased level of compleasomes in cerebrospinal fluid of patients with herpes simplex encephalitis
title_full_unstemmed Increased level of compleasomes in cerebrospinal fluid of patients with herpes simplex encephalitis
title_short Increased level of compleasomes in cerebrospinal fluid of patients with herpes simplex encephalitis
title_sort increased level of compleasomes in cerebrospinal fluid of patients with herpes simplex encephalitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6280959/
https://www.ncbi.nlm.nih.gov/pubmed/30094629
http://dx.doi.org/10.1007/s13365-018-0665-x
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