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Relationship of peripheral blood mononuclear cells miRNA expression and parasitic load in canine visceral leishmaniasis

Visceral leishmaniasis (VL) in humans is a chronic and often fatal disease if left untreated. Dogs appear to be the main reservoir host for L. infantum infection, however, in many regions other canids such as jackals, foxes, wolves and other mammals, such as hares or black rats, have been implicated...

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Autores principales: Bragato, Jaqueline Poleto, Melo, Larissa Martins, Venturin, Gabriela Lovizutto, Rebech, Gabriela Torres, Garcia, Leandro Encarnação, Lopes, Flavia Lombardi, de Lima, Valéria Marçal Felix
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6281177/
https://www.ncbi.nlm.nih.gov/pubmed/30517108
http://dx.doi.org/10.1371/journal.pone.0206876
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author Bragato, Jaqueline Poleto
Melo, Larissa Martins
Venturin, Gabriela Lovizutto
Rebech, Gabriela Torres
Garcia, Leandro Encarnação
Lopes, Flavia Lombardi
de Lima, Valéria Marçal Felix
author_facet Bragato, Jaqueline Poleto
Melo, Larissa Martins
Venturin, Gabriela Lovizutto
Rebech, Gabriela Torres
Garcia, Leandro Encarnação
Lopes, Flavia Lombardi
de Lima, Valéria Marçal Felix
author_sort Bragato, Jaqueline Poleto
collection PubMed
description Visceral leishmaniasis (VL) in humans is a chronic and often fatal disease if left untreated. Dogs appear to be the main reservoir host for L. infantum infection, however, in many regions other canids such as jackals, foxes, wolves and other mammals, such as hares or black rats, have been implicated as wild reservoirs. Most dogs cannot form an effective immune response against this infection, and this could be modulated by small non-coding RNAs, called microRNAs, responsible for post-transcriptional control of gene expression. Here, we evaluated the expression of miRNAs in peripheral blood mononuclear cells (PBMC) of symptomatic dogs naturally infected with Leishmania (L.) infantum (n = 10) and compared to those of healthy dogs (n = 5). Microarray analysis revealed that miR-21, miR-424, miR-194 and miR-451 had a 3-fold increase in expression, miR-192, miR-503, and miR-371 had a 2-fold increase in expression, whereas a 2-fold reduction in expression was observed for miR-150 and miR-574. Real-time PCR validated the differential expression of miR-21, miR-150, miR-451, miR-192, miR-194, and miR-371. Parasite load of PBMC was measured by real-time PCR and correlated to the differentially expressed miRNAs, showing a strong positive correlation with expression of miR-194, a regular positive correlation with miR-371 expression, and a moderate negative correlation with miR-150 expression in PBMC. These findings suggest that Leishmania infection interferes with miRNAs expression in PBMC, and their correlation with parasite load may help in the identification of therapeutic targets in Canine Visceral Leishmaniasis (CVL).
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spelling pubmed-62811772018-12-20 Relationship of peripheral blood mononuclear cells miRNA expression and parasitic load in canine visceral leishmaniasis Bragato, Jaqueline Poleto Melo, Larissa Martins Venturin, Gabriela Lovizutto Rebech, Gabriela Torres Garcia, Leandro Encarnação Lopes, Flavia Lombardi de Lima, Valéria Marçal Felix PLoS One Research Article Visceral leishmaniasis (VL) in humans is a chronic and often fatal disease if left untreated. Dogs appear to be the main reservoir host for L. infantum infection, however, in many regions other canids such as jackals, foxes, wolves and other mammals, such as hares or black rats, have been implicated as wild reservoirs. Most dogs cannot form an effective immune response against this infection, and this could be modulated by small non-coding RNAs, called microRNAs, responsible for post-transcriptional control of gene expression. Here, we evaluated the expression of miRNAs in peripheral blood mononuclear cells (PBMC) of symptomatic dogs naturally infected with Leishmania (L.) infantum (n = 10) and compared to those of healthy dogs (n = 5). Microarray analysis revealed that miR-21, miR-424, miR-194 and miR-451 had a 3-fold increase in expression, miR-192, miR-503, and miR-371 had a 2-fold increase in expression, whereas a 2-fold reduction in expression was observed for miR-150 and miR-574. Real-time PCR validated the differential expression of miR-21, miR-150, miR-451, miR-192, miR-194, and miR-371. Parasite load of PBMC was measured by real-time PCR and correlated to the differentially expressed miRNAs, showing a strong positive correlation with expression of miR-194, a regular positive correlation with miR-371 expression, and a moderate negative correlation with miR-150 expression in PBMC. These findings suggest that Leishmania infection interferes with miRNAs expression in PBMC, and their correlation with parasite load may help in the identification of therapeutic targets in Canine Visceral Leishmaniasis (CVL). Public Library of Science 2018-12-05 /pmc/articles/PMC6281177/ /pubmed/30517108 http://dx.doi.org/10.1371/journal.pone.0206876 Text en © 2018 Bragato et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Bragato, Jaqueline Poleto
Melo, Larissa Martins
Venturin, Gabriela Lovizutto
Rebech, Gabriela Torres
Garcia, Leandro Encarnação
Lopes, Flavia Lombardi
de Lima, Valéria Marçal Felix
Relationship of peripheral blood mononuclear cells miRNA expression and parasitic load in canine visceral leishmaniasis
title Relationship of peripheral blood mononuclear cells miRNA expression and parasitic load in canine visceral leishmaniasis
title_full Relationship of peripheral blood mononuclear cells miRNA expression and parasitic load in canine visceral leishmaniasis
title_fullStr Relationship of peripheral blood mononuclear cells miRNA expression and parasitic load in canine visceral leishmaniasis
title_full_unstemmed Relationship of peripheral blood mononuclear cells miRNA expression and parasitic load in canine visceral leishmaniasis
title_short Relationship of peripheral blood mononuclear cells miRNA expression and parasitic load in canine visceral leishmaniasis
title_sort relationship of peripheral blood mononuclear cells mirna expression and parasitic load in canine visceral leishmaniasis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6281177/
https://www.ncbi.nlm.nih.gov/pubmed/30517108
http://dx.doi.org/10.1371/journal.pone.0206876
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