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Plasmonic ELISA for Sensitive Detection of Disease Biomarkers with a Smart Phone-Based Reader
Serum myoglobin is one of the earliest markers for the diagnosis of acute myocardial infarction. It is, therefore, critical to develop a point-of-care testing technology for myoglobin detection. In this work, we reported a sensitive plasmonic immunoassay-based on enzyme-mediated localized surface pl...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6281541/ https://www.ncbi.nlm.nih.gov/pubmed/30519882 http://dx.doi.org/10.1186/s11671-018-2806-9 |
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author | Yang, Quanli Cai, Ruitian Xiao, Wei Wu, Zengfeng Liu, Xia Xu, Yan Xu, Miaomiao Zhong, Hui Sun, Guodong Liu, Qihui Fu, Qiangqiang Xiang, Junjian |
author_facet | Yang, Quanli Cai, Ruitian Xiao, Wei Wu, Zengfeng Liu, Xia Xu, Yan Xu, Miaomiao Zhong, Hui Sun, Guodong Liu, Qihui Fu, Qiangqiang Xiang, Junjian |
author_sort | Yang, Quanli |
collection | PubMed |
description | Serum myoglobin is one of the earliest markers for the diagnosis of acute myocardial infarction. It is, therefore, critical to develop a point-of-care testing technology for myoglobin detection. In this work, we reported a sensitive plasmonic immunoassay-based on enzyme-mediated localized surface plasmon resonance change of gold nanorods for the point-of-care testing detection of myoglobin. In addition, we developed a novel plasmonic immunoassay reader using the ambient light sensor of smart phone to increase the accessibility and utility of the plasmonic immunoassay. The linear detection range of gold nanorods-based plasmonic immunoassay for myoglobin detection was 0.1–1000 ng mL(−1) and the limit of detection was 0.057 ng mL(−1). Myoglobin in serum samples was also analyzed by the plasmonic immunoassay. The results were significantly correlated with those of conventional enzyme-linked immunosorbent assay. The plasmonic immunoassay, coupled with smart phone-based reader, could be widely used for point-of-care testing application of acute myocardial infarction, especially in the regions with limited technological resources. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s11671-018-2806-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6281541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-62815412018-12-26 Plasmonic ELISA for Sensitive Detection of Disease Biomarkers with a Smart Phone-Based Reader Yang, Quanli Cai, Ruitian Xiao, Wei Wu, Zengfeng Liu, Xia Xu, Yan Xu, Miaomiao Zhong, Hui Sun, Guodong Liu, Qihui Fu, Qiangqiang Xiang, Junjian Nanoscale Res Lett Nano Express Serum myoglobin is one of the earliest markers for the diagnosis of acute myocardial infarction. It is, therefore, critical to develop a point-of-care testing technology for myoglobin detection. In this work, we reported a sensitive plasmonic immunoassay-based on enzyme-mediated localized surface plasmon resonance change of gold nanorods for the point-of-care testing detection of myoglobin. In addition, we developed a novel plasmonic immunoassay reader using the ambient light sensor of smart phone to increase the accessibility and utility of the plasmonic immunoassay. The linear detection range of gold nanorods-based plasmonic immunoassay for myoglobin detection was 0.1–1000 ng mL(−1) and the limit of detection was 0.057 ng mL(−1). Myoglobin in serum samples was also analyzed by the plasmonic immunoassay. The results were significantly correlated with those of conventional enzyme-linked immunosorbent assay. The plasmonic immunoassay, coupled with smart phone-based reader, could be widely used for point-of-care testing application of acute myocardial infarction, especially in the regions with limited technological resources. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s11671-018-2806-9) contains supplementary material, which is available to authorized users. Springer US 2018-12-05 /pmc/articles/PMC6281541/ /pubmed/30519882 http://dx.doi.org/10.1186/s11671-018-2806-9 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Nano Express Yang, Quanli Cai, Ruitian Xiao, Wei Wu, Zengfeng Liu, Xia Xu, Yan Xu, Miaomiao Zhong, Hui Sun, Guodong Liu, Qihui Fu, Qiangqiang Xiang, Junjian Plasmonic ELISA for Sensitive Detection of Disease Biomarkers with a Smart Phone-Based Reader |
title | Plasmonic ELISA for Sensitive Detection of Disease Biomarkers with a Smart Phone-Based Reader |
title_full | Plasmonic ELISA for Sensitive Detection of Disease Biomarkers with a Smart Phone-Based Reader |
title_fullStr | Plasmonic ELISA for Sensitive Detection of Disease Biomarkers with a Smart Phone-Based Reader |
title_full_unstemmed | Plasmonic ELISA for Sensitive Detection of Disease Biomarkers with a Smart Phone-Based Reader |
title_short | Plasmonic ELISA for Sensitive Detection of Disease Biomarkers with a Smart Phone-Based Reader |
title_sort | plasmonic elisa for sensitive detection of disease biomarkers with a smart phone-based reader |
topic | Nano Express |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6281541/ https://www.ncbi.nlm.nih.gov/pubmed/30519882 http://dx.doi.org/10.1186/s11671-018-2806-9 |
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