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Sox2 is associated with cancer stem-like properties in colorectal cancer

Sox2 is known as the undifferentiated cell marker. Recent studies have shown that Sox2 may also be involved in the maintenance of cancer stem cells (CSCs) in skin and bladder cancers. In this study, we aimed to clarify the role of Sox2 in colorectal CSCs. Sox2 expression was measured in colon cancer...

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Autores principales: Takeda, Koki, Mizushima, Tsunekazu, Yokoyama, Yuhki, Hirose, Haruka, Wu, Xin, Qian, Yamin, Ikehata, Katsuya, Miyoshi, Norikatsu, Takahashi, Hidekazu, Haraguchi, Naotsugu, Hata, Taishi, Matsuda, Chu, Doki, Yuichiro, Mori, Masaki, Yamamoto, Hirofumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6281572/
https://www.ncbi.nlm.nih.gov/pubmed/30518951
http://dx.doi.org/10.1038/s41598-018-36251-0
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author Takeda, Koki
Mizushima, Tsunekazu
Yokoyama, Yuhki
Hirose, Haruka
Wu, Xin
Qian, Yamin
Ikehata, Katsuya
Miyoshi, Norikatsu
Takahashi, Hidekazu
Haraguchi, Naotsugu
Hata, Taishi
Matsuda, Chu
Doki, Yuichiro
Mori, Masaki
Yamamoto, Hirofumi
author_facet Takeda, Koki
Mizushima, Tsunekazu
Yokoyama, Yuhki
Hirose, Haruka
Wu, Xin
Qian, Yamin
Ikehata, Katsuya
Miyoshi, Norikatsu
Takahashi, Hidekazu
Haraguchi, Naotsugu
Hata, Taishi
Matsuda, Chu
Doki, Yuichiro
Mori, Masaki
Yamamoto, Hirofumi
author_sort Takeda, Koki
collection PubMed
description Sox2 is known as the undifferentiated cell marker. Recent studies have shown that Sox2 may also be involved in the maintenance of cancer stem cells (CSCs) in skin and bladder cancers. In this study, we aimed to clarify the role of Sox2 in colorectal CSCs. Sox2 expression was measured in colon cancer cells and colorectal clinical samples by qRT-PCR and western blot analysis. To visualize the active Sox2 mRNA production, we generated a Sox2 promoter-dependent DsRed fluorescence emission system. Colon cancer cell lines and colorectal tumor tissues generally expressed the Sox2 protein. Knockdown of Sox2 by siRNA led to increased proliferative activity in Caco2 cells. Kaplan-Meier survival curves showed that the group with high Sox2 mRNA expression had a worse prognosis for relapse-free survival (RFS) than the low expression group (P = 0.045, median follow-up 60.0 months). Time-lapse image analysis revealed that most DsRed(+) cells exhibited typical asymmetric cell division and had higher CSC marker expressions. The DsRed(+) cells exhibited chemoresistance and they grew slower in vitro, yet they established rather larger tumors in vivo. Our data suggest that Sox2 may be a potential biomarker for colorectal CSCs.
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spelling pubmed-62815722018-12-07 Sox2 is associated with cancer stem-like properties in colorectal cancer Takeda, Koki Mizushima, Tsunekazu Yokoyama, Yuhki Hirose, Haruka Wu, Xin Qian, Yamin Ikehata, Katsuya Miyoshi, Norikatsu Takahashi, Hidekazu Haraguchi, Naotsugu Hata, Taishi Matsuda, Chu Doki, Yuichiro Mori, Masaki Yamamoto, Hirofumi Sci Rep Article Sox2 is known as the undifferentiated cell marker. Recent studies have shown that Sox2 may also be involved in the maintenance of cancer stem cells (CSCs) in skin and bladder cancers. In this study, we aimed to clarify the role of Sox2 in colorectal CSCs. Sox2 expression was measured in colon cancer cells and colorectal clinical samples by qRT-PCR and western blot analysis. To visualize the active Sox2 mRNA production, we generated a Sox2 promoter-dependent DsRed fluorescence emission system. Colon cancer cell lines and colorectal tumor tissues generally expressed the Sox2 protein. Knockdown of Sox2 by siRNA led to increased proliferative activity in Caco2 cells. Kaplan-Meier survival curves showed that the group with high Sox2 mRNA expression had a worse prognosis for relapse-free survival (RFS) than the low expression group (P = 0.045, median follow-up 60.0 months). Time-lapse image analysis revealed that most DsRed(+) cells exhibited typical asymmetric cell division and had higher CSC marker expressions. The DsRed(+) cells exhibited chemoresistance and they grew slower in vitro, yet they established rather larger tumors in vivo. Our data suggest that Sox2 may be a potential biomarker for colorectal CSCs. Nature Publishing Group UK 2018-12-05 /pmc/articles/PMC6281572/ /pubmed/30518951 http://dx.doi.org/10.1038/s41598-018-36251-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Takeda, Koki
Mizushima, Tsunekazu
Yokoyama, Yuhki
Hirose, Haruka
Wu, Xin
Qian, Yamin
Ikehata, Katsuya
Miyoshi, Norikatsu
Takahashi, Hidekazu
Haraguchi, Naotsugu
Hata, Taishi
Matsuda, Chu
Doki, Yuichiro
Mori, Masaki
Yamamoto, Hirofumi
Sox2 is associated with cancer stem-like properties in colorectal cancer
title Sox2 is associated with cancer stem-like properties in colorectal cancer
title_full Sox2 is associated with cancer stem-like properties in colorectal cancer
title_fullStr Sox2 is associated with cancer stem-like properties in colorectal cancer
title_full_unstemmed Sox2 is associated with cancer stem-like properties in colorectal cancer
title_short Sox2 is associated with cancer stem-like properties in colorectal cancer
title_sort sox2 is associated with cancer stem-like properties in colorectal cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6281572/
https://www.ncbi.nlm.nih.gov/pubmed/30518951
http://dx.doi.org/10.1038/s41598-018-36251-0
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