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Boolean Calcium Signalling Model Predicts Calcium Role in Acceleration and Stability of Abscisic Acid-Mediated Stomatal Closure
Inconsistent hypotheses have proposed Ca(2+) as either being essential or irrelevant and redundant in ABA induced stomatal closure. This study integrates all available information from literature to define ABA signalling pathway and presents it in a systems view for clearer understanding of the role...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6281740/ https://www.ncbi.nlm.nih.gov/pubmed/30518777 http://dx.doi.org/10.1038/s41598-018-35872-9 |
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author | Waidyarathne, Pramuditha Samarasinghe, Sandhya |
author_facet | Waidyarathne, Pramuditha Samarasinghe, Sandhya |
author_sort | Waidyarathne, Pramuditha |
collection | PubMed |
description | Inconsistent hypotheses have proposed Ca(2+) as either being essential or irrelevant and redundant in ABA induced stomatal closure. This study integrates all available information from literature to define ABA signalling pathway and presents it in a systems view for clearer understanding of the role of Ca(2+) in stomatal closure. Importantly, it incorporates into an Asynchronous Boolean model time delays sourced from an extensive literature search. The model predicted the timing of ABA events and mutant behaviour close to biology. It revealed biologically reported timing for Ca(2+) activation and Ca(2+) dynamics consistent with biology. It also predicts that Ca(2+) elevation is not essential in stomatal closure but it can accelerate closure, consistent with previous findings, but our model further explains that acting as a mediator, Ca(2+) accelerates stomatal closure by enhancing plasma membrane slowly activating anion channel SLAC1 and actin rearrangement. It shows statistical significance of Ca(2+) induced acceleration of closure and that of Ca(2+) induced acceleration of SLAC1 activation. Further, the model demonstrates that Ca(2+) enhances resilience of closure to perturbation of important elements; especially, ROS pathway, as did previous ABA model, and even to the ABA signal disruption. It goes further to elucidate the mechanisms by which Ca(2+) engenders stomatal closure in these perturbations. |
format | Online Article Text |
id | pubmed-6281740 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62817402018-12-07 Boolean Calcium Signalling Model Predicts Calcium Role in Acceleration and Stability of Abscisic Acid-Mediated Stomatal Closure Waidyarathne, Pramuditha Samarasinghe, Sandhya Sci Rep Article Inconsistent hypotheses have proposed Ca(2+) as either being essential or irrelevant and redundant in ABA induced stomatal closure. This study integrates all available information from literature to define ABA signalling pathway and presents it in a systems view for clearer understanding of the role of Ca(2+) in stomatal closure. Importantly, it incorporates into an Asynchronous Boolean model time delays sourced from an extensive literature search. The model predicted the timing of ABA events and mutant behaviour close to biology. It revealed biologically reported timing for Ca(2+) activation and Ca(2+) dynamics consistent with biology. It also predicts that Ca(2+) elevation is not essential in stomatal closure but it can accelerate closure, consistent with previous findings, but our model further explains that acting as a mediator, Ca(2+) accelerates stomatal closure by enhancing plasma membrane slowly activating anion channel SLAC1 and actin rearrangement. It shows statistical significance of Ca(2+) induced acceleration of closure and that of Ca(2+) induced acceleration of SLAC1 activation. Further, the model demonstrates that Ca(2+) enhances resilience of closure to perturbation of important elements; especially, ROS pathway, as did previous ABA model, and even to the ABA signal disruption. It goes further to elucidate the mechanisms by which Ca(2+) engenders stomatal closure in these perturbations. Nature Publishing Group UK 2018-12-05 /pmc/articles/PMC6281740/ /pubmed/30518777 http://dx.doi.org/10.1038/s41598-018-35872-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Waidyarathne, Pramuditha Samarasinghe, Sandhya Boolean Calcium Signalling Model Predicts Calcium Role in Acceleration and Stability of Abscisic Acid-Mediated Stomatal Closure |
title | Boolean Calcium Signalling Model Predicts Calcium Role in Acceleration and Stability of Abscisic Acid-Mediated Stomatal Closure |
title_full | Boolean Calcium Signalling Model Predicts Calcium Role in Acceleration and Stability of Abscisic Acid-Mediated Stomatal Closure |
title_fullStr | Boolean Calcium Signalling Model Predicts Calcium Role in Acceleration and Stability of Abscisic Acid-Mediated Stomatal Closure |
title_full_unstemmed | Boolean Calcium Signalling Model Predicts Calcium Role in Acceleration and Stability of Abscisic Acid-Mediated Stomatal Closure |
title_short | Boolean Calcium Signalling Model Predicts Calcium Role in Acceleration and Stability of Abscisic Acid-Mediated Stomatal Closure |
title_sort | boolean calcium signalling model predicts calcium role in acceleration and stability of abscisic acid-mediated stomatal closure |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6281740/ https://www.ncbi.nlm.nih.gov/pubmed/30518777 http://dx.doi.org/10.1038/s41598-018-35872-9 |
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