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Overexpression of Acyl-CoA-Binding Protein 1 (ChACBP1) From Saline-Alkali-Tolerant Chlorella sp. Enhances Stress Tolerance in Arabidopsis
A large proportion of the world’s arable land is saline-alkali land, and this is becoming an urgent environmental problem for agriculture. One approach to address this problem is to develop new varieties of stress-resistant plants through genetic engineering. The algae (Chlorella sp.) JB6, which was...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282033/ https://www.ncbi.nlm.nih.gov/pubmed/30555504 http://dx.doi.org/10.3389/fpls.2018.01772 |
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author | Qiao, Kun Wang, Min Takano, Tetsuo Liu, Shenkui |
author_facet | Qiao, Kun Wang, Min Takano, Tetsuo Liu, Shenkui |
author_sort | Qiao, Kun |
collection | PubMed |
description | A large proportion of the world’s arable land is saline-alkali land, and this is becoming an urgent environmental problem for agriculture. One approach to address this problem is to develop new varieties of stress-resistant plants through genetic engineering. The algae (Chlorella sp.) JB6, which was previously isolated from saline-alkali land, was found to exhibit strong NaHCO(3) tolerance. Here, we explored saline-alkali-tolerance genes in this alga that might be useful for producing abiotic stress-resistant transgenic plants. We identified a gene encoding acyl-CoA-binding protein 1 (ACBP1) from JB6 by screening a full-length cDNA library in yeast under NaHCO(3) stress. Northern blot analyses showed that the ChACBP1 mRNA levels were significantly up-regulated under abiotic stresses such as salinity, oxidation, heavy metals, and low temperature stresses. The recombinant ChACBP1 protein was found to bind phosphatidylcholine in vitro. Green fluorescent protein-labeled ChACBP1 was localized to the cytosol. Overexpression of ChACBP1 in yeast and Arabidopsis increased their resistance to high salinity, oxidation, heavy metals, and low temperature stresses. These results suggested that ChACBP1 may mediate plant abiotic stress adaptation through phospholipid metabolism. Thus, ChACBP1 may be useful to genetically improve the tolerance of plants to saline-alkali soil. |
format | Online Article Text |
id | pubmed-6282033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62820332018-12-14 Overexpression of Acyl-CoA-Binding Protein 1 (ChACBP1) From Saline-Alkali-Tolerant Chlorella sp. Enhances Stress Tolerance in Arabidopsis Qiao, Kun Wang, Min Takano, Tetsuo Liu, Shenkui Front Plant Sci Plant Science A large proportion of the world’s arable land is saline-alkali land, and this is becoming an urgent environmental problem for agriculture. One approach to address this problem is to develop new varieties of stress-resistant plants through genetic engineering. The algae (Chlorella sp.) JB6, which was previously isolated from saline-alkali land, was found to exhibit strong NaHCO(3) tolerance. Here, we explored saline-alkali-tolerance genes in this alga that might be useful for producing abiotic stress-resistant transgenic plants. We identified a gene encoding acyl-CoA-binding protein 1 (ACBP1) from JB6 by screening a full-length cDNA library in yeast under NaHCO(3) stress. Northern blot analyses showed that the ChACBP1 mRNA levels were significantly up-regulated under abiotic stresses such as salinity, oxidation, heavy metals, and low temperature stresses. The recombinant ChACBP1 protein was found to bind phosphatidylcholine in vitro. Green fluorescent protein-labeled ChACBP1 was localized to the cytosol. Overexpression of ChACBP1 in yeast and Arabidopsis increased their resistance to high salinity, oxidation, heavy metals, and low temperature stresses. These results suggested that ChACBP1 may mediate plant abiotic stress adaptation through phospholipid metabolism. Thus, ChACBP1 may be useful to genetically improve the tolerance of plants to saline-alkali soil. Frontiers Media S.A. 2018-11-28 /pmc/articles/PMC6282033/ /pubmed/30555504 http://dx.doi.org/10.3389/fpls.2018.01772 Text en Copyright © 2018 Qiao, Wang, Takano and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Qiao, Kun Wang, Min Takano, Tetsuo Liu, Shenkui Overexpression of Acyl-CoA-Binding Protein 1 (ChACBP1) From Saline-Alkali-Tolerant Chlorella sp. Enhances Stress Tolerance in Arabidopsis |
title | Overexpression of Acyl-CoA-Binding Protein 1 (ChACBP1) From Saline-Alkali-Tolerant Chlorella sp. Enhances Stress Tolerance in Arabidopsis |
title_full | Overexpression of Acyl-CoA-Binding Protein 1 (ChACBP1) From Saline-Alkali-Tolerant Chlorella sp. Enhances Stress Tolerance in Arabidopsis |
title_fullStr | Overexpression of Acyl-CoA-Binding Protein 1 (ChACBP1) From Saline-Alkali-Tolerant Chlorella sp. Enhances Stress Tolerance in Arabidopsis |
title_full_unstemmed | Overexpression of Acyl-CoA-Binding Protein 1 (ChACBP1) From Saline-Alkali-Tolerant Chlorella sp. Enhances Stress Tolerance in Arabidopsis |
title_short | Overexpression of Acyl-CoA-Binding Protein 1 (ChACBP1) From Saline-Alkali-Tolerant Chlorella sp. Enhances Stress Tolerance in Arabidopsis |
title_sort | overexpression of acyl-coa-binding protein 1 (chacbp1) from saline-alkali-tolerant chlorella sp. enhances stress tolerance in arabidopsis |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282033/ https://www.ncbi.nlm.nih.gov/pubmed/30555504 http://dx.doi.org/10.3389/fpls.2018.01772 |
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