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The Phosphohistidine Phosphatase SixA Targets a Phosphotransferase System

SixA, a well-conserved protein found in proteobacteria, actinobacteria, and cyanobacteria, is the only reported example of a bacterial phosphohistidine phosphatase. A single protein target of SixA has been reported to date: the Escherichia coli histidine kinase ArcB. The present work analyzes an Arc...

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Autores principales: Schulte, Jane E., Goulian, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282199/
https://www.ncbi.nlm.nih.gov/pubmed/30482831
http://dx.doi.org/10.1128/mBio.01666-18
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author Schulte, Jane E.
Goulian, Mark
author_facet Schulte, Jane E.
Goulian, Mark
author_sort Schulte, Jane E.
collection PubMed
description SixA, a well-conserved protein found in proteobacteria, actinobacteria, and cyanobacteria, is the only reported example of a bacterial phosphohistidine phosphatase. A single protein target of SixA has been reported to date: the Escherichia coli histidine kinase ArcB. The present work analyzes an ArcB-independent growth defect of a sixA deletion in E. coli. A screen for suppressors, analysis of various mutants, and phosphorylation assays indicate that SixA modulates phosphorylation of the nitrogen-related phosphotransferase system (PTS(Ntr)). The PTS(Ntr) is a widely conserved bacterial pathway that regulates diverse metabolic processes through the phosphorylation states of its protein components, EI(Ntr), NPr, and EIIA(Ntr), which receive phosphoryl groups on histidine residues. However, a mechanism for dephosphorylating this system has not been reported. The results presented here suggest a model in which SixA removes phosphoryl groups from the PTS(Ntr) by acting on NPr. This work uncovers a new role for the phosphohistidine phosphatase SixA and, through factors that affect SixA expression or activity, may point to additional inputs that regulate the PTS(Ntr).
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spelling pubmed-62821992018-12-10 The Phosphohistidine Phosphatase SixA Targets a Phosphotransferase System Schulte, Jane E. Goulian, Mark mBio Research Article SixA, a well-conserved protein found in proteobacteria, actinobacteria, and cyanobacteria, is the only reported example of a bacterial phosphohistidine phosphatase. A single protein target of SixA has been reported to date: the Escherichia coli histidine kinase ArcB. The present work analyzes an ArcB-independent growth defect of a sixA deletion in E. coli. A screen for suppressors, analysis of various mutants, and phosphorylation assays indicate that SixA modulates phosphorylation of the nitrogen-related phosphotransferase system (PTS(Ntr)). The PTS(Ntr) is a widely conserved bacterial pathway that regulates diverse metabolic processes through the phosphorylation states of its protein components, EI(Ntr), NPr, and EIIA(Ntr), which receive phosphoryl groups on histidine residues. However, a mechanism for dephosphorylating this system has not been reported. The results presented here suggest a model in which SixA removes phosphoryl groups from the PTS(Ntr) by acting on NPr. This work uncovers a new role for the phosphohistidine phosphatase SixA and, through factors that affect SixA expression or activity, may point to additional inputs that regulate the PTS(Ntr). American Society for Microbiology 2018-11-27 /pmc/articles/PMC6282199/ /pubmed/30482831 http://dx.doi.org/10.1128/mBio.01666-18 Text en Copyright © 2018 Schulte and Goulian. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Schulte, Jane E.
Goulian, Mark
The Phosphohistidine Phosphatase SixA Targets a Phosphotransferase System
title The Phosphohistidine Phosphatase SixA Targets a Phosphotransferase System
title_full The Phosphohistidine Phosphatase SixA Targets a Phosphotransferase System
title_fullStr The Phosphohistidine Phosphatase SixA Targets a Phosphotransferase System
title_full_unstemmed The Phosphohistidine Phosphatase SixA Targets a Phosphotransferase System
title_short The Phosphohistidine Phosphatase SixA Targets a Phosphotransferase System
title_sort phosphohistidine phosphatase sixa targets a phosphotransferase system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282199/
https://www.ncbi.nlm.nih.gov/pubmed/30482831
http://dx.doi.org/10.1128/mBio.01666-18
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