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Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets

BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in humans, cows, and pigs. The gut microbiota underlies pathology of several infectious diseases yet the role of the gut microbiota in the pathogenesis of ETEC-induced diarrhea is unknown. RESULTS: By using an ETEC induced diarrheal...

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Autores principales: Bin, Peng, Tang, Zhiyi, Liu, Shaojuan, Chen, Shuai, Xia, Yaoyao, Liu, Jiaqi, Wu, Hucong, Zhu, Guoqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282381/
https://www.ncbi.nlm.nih.gov/pubmed/30518356
http://dx.doi.org/10.1186/s12917-018-1704-9
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author Bin, Peng
Tang, Zhiyi
Liu, Shaojuan
Chen, Shuai
Xia, Yaoyao
Liu, Jiaqi
Wu, Hucong
Zhu, Guoqiang
author_facet Bin, Peng
Tang, Zhiyi
Liu, Shaojuan
Chen, Shuai
Xia, Yaoyao
Liu, Jiaqi
Wu, Hucong
Zhu, Guoqiang
author_sort Bin, Peng
collection PubMed
description BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in humans, cows, and pigs. The gut microbiota underlies pathology of several infectious diseases yet the role of the gut microbiota in the pathogenesis of ETEC-induced diarrhea is unknown. RESULTS: By using an ETEC induced diarrheal model in piglet, we profiled the jejunal and fecal microbiota using metagenomics and 16S rRNA sequencing. A jejunal microbiota transplantation experiment was conducted to determine the role of the gut microbiota in ETEC-induced diarrhea. ETEC-induced diarrhea influenced the structure and function of gut microbiota. Diarrheal piglets had lower Bacteroidetes: Firmicutes ratio and microbiota diversity in the jejunum and feces, and lower percentage of Prevotella in the feces, but higher Lactococcus in the jejunum and higher Escherichia-Shigella in the feces. The transplantation of the jejunal microbiota from diarrheal piglets to uninfected piglets leaded to diarrhea after transplantation. Microbiota transplantation experiments also supported the notion that dysbiosis of gut microbiota is involved in the immune responses in ETEC-induced diarrhea. CONCLUSION: We conclude that ETEC infection influences the gut microbiota and the dysbiosis of gut microbiota after ETEC infection mediates the immune responses in ETEC infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12917-018-1704-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-62823812018-12-10 Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets Bin, Peng Tang, Zhiyi Liu, Shaojuan Chen, Shuai Xia, Yaoyao Liu, Jiaqi Wu, Hucong Zhu, Guoqiang BMC Vet Res Research Article BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in humans, cows, and pigs. The gut microbiota underlies pathology of several infectious diseases yet the role of the gut microbiota in the pathogenesis of ETEC-induced diarrhea is unknown. RESULTS: By using an ETEC induced diarrheal model in piglet, we profiled the jejunal and fecal microbiota using metagenomics and 16S rRNA sequencing. A jejunal microbiota transplantation experiment was conducted to determine the role of the gut microbiota in ETEC-induced diarrhea. ETEC-induced diarrhea influenced the structure and function of gut microbiota. Diarrheal piglets had lower Bacteroidetes: Firmicutes ratio and microbiota diversity in the jejunum and feces, and lower percentage of Prevotella in the feces, but higher Lactococcus in the jejunum and higher Escherichia-Shigella in the feces. The transplantation of the jejunal microbiota from diarrheal piglets to uninfected piglets leaded to diarrhea after transplantation. Microbiota transplantation experiments also supported the notion that dysbiosis of gut microbiota is involved in the immune responses in ETEC-induced diarrhea. CONCLUSION: We conclude that ETEC infection influences the gut microbiota and the dysbiosis of gut microbiota after ETEC infection mediates the immune responses in ETEC infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12917-018-1704-9) contains supplementary material, which is available to authorized users. BioMed Central 2018-12-05 /pmc/articles/PMC6282381/ /pubmed/30518356 http://dx.doi.org/10.1186/s12917-018-1704-9 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Bin, Peng
Tang, Zhiyi
Liu, Shaojuan
Chen, Shuai
Xia, Yaoyao
Liu, Jiaqi
Wu, Hucong
Zhu, Guoqiang
Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets
title Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets
title_full Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets
title_fullStr Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets
title_full_unstemmed Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets
title_short Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets
title_sort intestinal microbiota mediates enterotoxigenic escherichia coli-induced diarrhea in piglets
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282381/
https://www.ncbi.nlm.nih.gov/pubmed/30518356
http://dx.doi.org/10.1186/s12917-018-1704-9
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